DS 21.212-DS 21.213 DYE STAINS OP SPECIAL APPLICATION 409 



method: [celloidin sections of F 4700.0010 Wolters (1890) fixed material] —> water — » A, 

 24 hrs., 50°C. -> wash -^ B, 24 hrs., 50°C. -^ C, till differentiated -* balsam, via 

 usual reagents 



RECOMMENDED FOR: myelin sheaths. 



note: Solution A, above, is not Wolters' original mordant for which see ADS 12.1 

 Wolters 1891. 



21.212 Wright test. 1938 Mallory Mallory 1938, 236 

 RE.vGENTS REQUIRED: A. 10% ferric chloride; B. 0.02% hematoxylin 



method: [frozen sections varnished to slide with collodion] —> A, 5 mins. — > blot — > B, 



poured on slide, 30 mins. — > A, till differentiated — * balsam, via usual reagents 

 RECOMMENDED FOR: myelin sheaths. 



21.213 Other Methods 



So deeply have the hematoxylin methods, given in the last section, become embedded 

 in the literature, that it is almost impossible for an author today to suggest the use of 

 any other dye in the staining of nervous elements after mordanting. Particular atten- 

 tion should tlierefore be drawn to the formula of Becher 1921, which is permanent, easy, 

 and simple, and which gives an admirable picture of the nervous elements in a general 

 brain structure. It does not give so sharp a differentiation, either as a silver method, or a 

 well-differentiated hematoxylin one, but it has at least the advantage of providing a 

 permanent, simple, one-solution metliod which can with safety be placed in the hands 

 of a beginner. Attention must also be drawn to the formula of Romeis 1922, which is 

 designed to stain specifically the nervous elements in wholemounts of small inverte- 

 brates. Tlie stain is certain, and completely specific, but it has the unfortunate dis- 

 advantage that no method has yet been found by which it can be rendered permanent. 

 It should, however, be placed in the hands of any class studying small invertebrates, 

 particularly fresh-water oligochaetes, for it is fascinating to a student to watch the 

 development of the nervous structures as they become clearer and clearer. It is also, of 

 course, of immense value in cases in which the morphology of the nervous system plays 

 any part in the taxonomy of an invertebrate. 



21.213 Adamkiewicz test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 165 

 REAGENTS REQUIRED: A. 0.01% nitric acid; B. sat. aq. sol. safranin O; C. 0.01% nitric 



acid in abs. ale. 

 method: [sections] -^ A, rinse -^ B, 6-12 hrs. -^ abs. ale, rinse -^ C, rinse — > clove oil, 



till differentiated — > balsam 

 RECOMMENDED FOR: general neurological staining. 



21.213 Alzheimer 1910 Nissl and Alzheimer 1910, 410 



REAGENTS REQUIRED: A. 4% formaldehyde; B. F 1600.0010 Flemming 1882; C. sat. aq. 



sol. acid fuchsin; D. 0.6% picric acid; E. sat. aq. sol. light green 

 method: [fresh tissue] -^ A, 2\ hrs. — * 5, 8 days -^ wash -^ [2-3 ti paraffin sections] -^ 



95% ale. -^ C, 1 hr. at 60°C. — > wash till no more color comes away — > D, 15-20 sees. 



-^ rinse twice -^ E, 20-50 mins. — > balsam via usual reagents 

 RECOMMENDED FOR: general neurological staining. 

 note: See also DS 22.8 Alzheimer 1910. 



21.213 Aronson 1890 23730, 28:577 



REAGENTS REQUIRED: A. F 7000.0000 Mtlller 1859; B. 2% copper acetate; C. water 85, 



95% ale. 15, sodium carbonate 0.03, gallein to sat.; D. ADS 21.1 Weigert 1885 

 method: [whole brains] —> A, till hardened —> [15 mm. slices]—^ B, 1 wk. — > wash — > 



[12-25 n sections] — > C, 24 hrs. — * rinse —* D, till differentiated -^ balsam, via usual 



reagents 

 note: Erlitzky 1877 F 4700.0000 may replace A, with the consequent omission of B. 



21.213 Becher 1921 see DS 13.5 Becher 1921c 



