410 METHODS AND FORMULAS DS 21.213 



21.213 Boissezon 1941 4285a, 18:90 



REAGENTS REQUIRED: A, acetone; B. sat. 70% ale. sol. Sudan black B 



method: [frozen sections]^ A, 15 mins. -^ 70% ale, 10 mins. — > B, 5 mins. — > 60% 



ale, till differentiated -^^ M 12.1 mountant 

 RECOMMENDED FOR: myelin of peripheral nerves. 



21.213 Bretschneider 1914 10899, 52:271 



REAGENTS REQUIRED: .1. 1% eosin; B. DS 11.122 Delafield (1885); C. 1% phosphomolyb- 



dic acid; D. DS 13.41 Mallory 1901 (sol. C) 

 method: [sections of formaldehyde-fixed material] — » water —> A, 20 mins. —* wash -^ 



B, 30 sees. -^ wash -^ C, 2-3 mins. — > wash -^ D, 10 sees. -^ wash — + balsam, via 



usual reagents 

 RECOMMENDED FOR: insect brains. 



21.213 Campbell 1929 .see DS 23.223 Campbell 1929 



21.213 Chilesotti test. Bohm and Oppel cit. Schmaus Bohm and Oppel 1907, 289 



REAGENTS REQUIRED: A. sodium camiinate 1, uranium nitrate 0.1, water 100, hydro- 

 chloric acid 0.03; B. 0.5% hydrochloric acid; C. F 7000.0000 MiUler 1859 

 method: [sections of chrome, or dichromate, fixed material] -^ water -^ A, 1 hr. -^ B, 



till differentiated — » wash — > C, 1 hr. -^ wash — * balsam, via usual reagents 

 RECOMMENDED FOR: axis cylinders. 



21.213 Eberspache 1936 .see ADS 22.1 Eberspache 193G 



21.213 Gross 1952 Tech. Bull., 22:234 



STOCK solutions: I. 4% magenta in 95% ale; II. 6% phenol; III. 1.5% methylene blue 



in 95%, ale. 

 REAGENTS REQUIRED: A. stock I 25, stock II 75, tergitol 7 0.1; 5. 3% hydrochloric acid 



in 95% ale.; C. water 100, stock III 30, potassium hydroxide 0.01 

 method: [smears] -^ A, 5-10 mins. — > rinse—* B, ^ to 1 min. with agitation — > rinse 



-^ C, 3-5 mins. -^ rinse -^ dry 



21.213 Lison and Dagnelle 1935 4285a, 12 :85 



reagents required: .1. sat. 70% ale. sol. Sudan black B 

 method: [30 m frozen sections of formaldehyde-fixed material] — > water -^ 70% ale. -^ 



— > A, 1-12 hrs. -^ 50% ale, till differentiated—* water -^ M 12.1 mountant 

 recommended for: myelin sheaths. 



21.213 McManns 1946 see DS 22.21 McManns 1946 



21.213 Menner 1935 23822,110:200 



stock solution: water 100, oxalic acid 2, potassium antimony tartrate 0.5, azoaeid 



blue B 1. Boil; cool. Filter. 

 reagents required: A. 0.005% chromic acid; B. water 96, stock .solution 4 

 method: [pieces of fresh spinal cord] -^ A, 2-8 days -^ rinse — > B, 24 hrs. -^ rinse ^ 



glycerol, via graded glycerol-water series 

 recommended for: ganglion cells in wholemounts. 



21.213 Nikiforoff /< s/. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 166 



REAGENTS REQUIRED: A. sat. sol. safrauiu in sat. sol. aniline; B. 0.2% gold chloride 

 method: [.sections of chrome-fixed materials] -^ A, 24 hrs. —> 95% ale, till gray matter 

 begins to differentiate from white -^ B, till violet —>■ thorough wash -^ 95% ale, till 

 gray matter clearly differentiated from white — > balsam, via clove oil 

 recommended for: general neurological staining. 



21.213 Nissl test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 156 



reagents required: sat. aq. sol. magenta 

 method: [sections] — > stain, on slide, heated to steaming, few moments -* abs. ale, till 



color clouds cease — > oil of cloves, till differentiated -^ balsam, via xylene 

 recommended for: nerve cells and processes. 



