DS 21.213-DS 21.22 DYE STAINS OF SPECIAL APPLICATION 411 



21.213 Obersteiner Icsl. 1896 Kahlden and Laurent Kahklon and Lament 1896, 175 



REAGENTS REyiiKKi): .1. 20% ferric cliloride; U. sat. sol. dinitroresoroinol in 75% ale. 

 method: [pieces of nerve trunks] —* A, 1 to 2 days -^ wash, till wash water iron-free — » 



B, several wks. — ' [paraffin sections] 

 result: axis cylinders bright green. 



21.213 O'Leary test. 1943 Cowdry Cowdry 1943, 144 



REAGE.vTs REQUIRED: .1 . 3% potassium dichromatc or F 7000.0000 Miillcr 1859; B. water 



100, acetic acid 0.2, abs. ale. 10, brazilin 1; C. 0.25% potassium permanganate; D. 



ADS 21.1 Weil 1928b 

 FREPAR.\TiON OF b: Dissolve the dye in ale. Allow to "ripen" 1-6 months. Add other 



ingredients. 

 method: [celloidiii sections of F 7000.0000 Miiller 1859 fixed ti.ssue] — » water -^ A, 12-24 



hrs. — » B, till deeply stained —> water, wash — > C, till differentiated, 1-5 mins. — > D, 



till permanganate decolorized — * water, wash — » balsam, via usual reagents 



21.213 Prince 1952 4349, 11:55 



formula: sat. aq. magenta 6, 1% erythrosin 17.5, sat. aq. sol. methyl orange 47, sat. 



aq. sol. anilin blue 29.5 

 method: [sections of formaldehyde-fixed material] -* water ^ stain, 10-30 sees. — » 



wash — > abs. ale. — > balsam, via xylene 



21.213 Romeis 1922 6628, 175:455 



REAGENTS REQUIRED: A. Water 100, benzidine 1, acetic acid 0.1; B. "12 vol." hydrogen 



peroxide 

 method: [small invertebrates, either living or alcohol-fixed] —> A, Yi to 1 hr. -^ B, 



dropped directly on animal on slide — * examine 

 recommended for: demonstration of nervous system of small invertebrates. 

 note: The stain is specific but fugitive. 



21.213 Schrotter 1902 15058, 21 :338 



reagents required: A. water 100, alizarin red S 5, oxalic acid 0.01; B. water 100, 



sodium carbonate 0.3 

 method: [sections] -^ A, 2-3 hrs. -^ B, till no more color comes away — > balsam, via 



usual reagents 



21.213 Tress and Tress 1935 20540b, 10:105 



reagents required: A. water 100, acetic acid 0.01, cresyl violet 0.5; B. chloroform 75, 

 abs. ale. 12.5, ether 12.5; C. 0.0025 hydrochloric acid in 95% ale; D. 0.01% sodium 

 bicarbonate in 95% ale. 

 method: [celloidin sections of formaldehyde-fixed material] —> water —>• A, 30 mins. 

 50°C. -^ wash^ 70% ale., till celloidin stain-free^ B, 2-5 mins. C, till differenti- 

 ated -♦ -D, till neutralized ^95% ale, wash -^ balsam, via n-butyl ale. 



21.213 Verne 1928 4285a, 5:223 



REAGENTS REQUIRED: A. DS 11.44 Schiff 1866; B. water 100, sodium metabisulfite 0.5, 



hydrochloric acid 5 

 method: [frozen sections of mercuric or platinie fixed nerves] -* 90% ale, 10 mins. — ♦ 



A, till deep red -* B, wash in several changes -> rinse -^ M 11.1 Apdthy 1892 

 recommended for: selective staining of myelin. 



21.22 METHODS FOR NEUROGLIA 



The crystal violet and victoria blue methods for staining the supporting elements of the 

 nervous system are less specific than are the metal stains. They still occur with some fre- 

 quency in the literature, however, and are warmly advocated by many technicians. 



21.22 Alzheimer 1910a te&i. 1938 Mallory Mallory 1938, 245 



reagents required: A. F 1600.0010 Flemming 1882; B. sat. aq. sol. acid fuehsin; C. 3% 



picric acid in 30% ale; D. 10% light green 

 method: [thin slices of formaldehyde-fixed material]-* A, 8 days in dark-* wash ^ 



[2-4 M paraffin sections] -^ B,\ hr., 60°C. -^ wash -^ C, few sees, to 2 mins. -^ wash 



-^ D, }>2 to 1 hr. — > wash — * 95% ale, till violet — > abs. ale, minimum possible time 



— > balsam, via xylene 



