412 METHODS AND FORMULAS DS 21.22 



21.22 Alzheimer 1910b test. 1938 Mallory Mallory 1938, 245 



REAGENTS REQUIRED: A. ADS 12.1 Wcigert 1891 90; B. sat. sol., {circ. 60%) phospho- 



molybdic acid, 40% formaldehyde 10; C. DS 13.7 Mann 1892a 

 method: [slices of fresh tissue] — > A, 2 wks. — + wash -^ [10 n frozen sections] -^ B, 2-12 

 hrs. — > quick wash -^ C, 1-5 hrs. — > water, till color clouds cease -^ 95% ale, till 

 gray matter blue and white matter pink -^^ abs. ale. minimum possible time — > bal- 

 sam, via xylene 



21.22 Alzheimer 1910c test. 1938 Mallory Mallory 1938, 245 



reagents required: A. ADS 12.1 Weigert 1891; B. 0.5%, acetic acid; G. DS 11.124 



Mallory 1891 3, water 97 

 method: [slices of fresh tissue] — > A, 2 wks. — » wash — » [10-12 ju frozen sections] -^ B, 



2 mins. —* C, 2 mins. — > rinse — » balsam, via usual reagents 



21.22 Anderson 1923 11431, 26:431 



reagents required: A. F 5000.1010 Bouin 1897; B, ADS 12.2 Anderson 1923 stock 



A 65, stock B 35; C, ADS 21.1 Pal 1887 (A & B sols.); D. 1.5%, Victoria blue ; E. ADS 



12.2 Lugol (1905); F. aniline 50, xylene 50 

 method: [frozen sections of formaldehyde-fixed material]—* A, overnight —> 70% ale, 



wash — > B, 10-30 mins. — + wash — * C, {A sol.), 5 mins. —* C {B sol.), 5 mins. or until 



next required -^ [sections attached to slide] -^ D, flooded on slide while boiling, 5 



mins. — » drain — > E, dropped on slide 1 min. — > F, 15 sees. -* xylene, till clear — > F, 



till differentiated -^ balsam, via xylene 

 note: Anderson later (Anderson 1929, 133) recommended equal parts stock A and 



stock B for mixture B above. 



21.22 Anglade and Morel 1901 19219,9:137 



reagents required: A. 6% mercuric chloride; B. F 1360.0010 Galescu 1908; C. sat. 



sol. {circ. 4%o) Victoria blue; D. ADS 12.2 Lugol (1905); E. xylene 35, aniline 65 

 method: [pieces of tissue]—* A, 5 hrs. -^ B, 12 hrs., 37°C. — > B, (fresh sol.), 12 hrs. 



37°C. -^5, (fresh sol.) 24 hrs. 37°C. -^ wash -> [paraffin sections] -^ water -> C, 



poured on slide and heated to steaming -^ D, used to wash C from slide — * E, till 



differentiated — + balsam, via xylene 



21.22 Bailey 1923 11343, 44:73 



formula of dry stock: water 100, orange G 0.5, ethyl violet 1 

 preparation dry stock: Digest 1 day 40°C. Filter. Wash and dry filtrate. 

 preparation of stock solution: 95% ale. 100, dry powder from above to sat. 

 reagents required: A. ADS 12.2 Gram 1884; B. 3% potassium dichromate; C. water 



60, 96% ale. 15, stock solution 25; D. clove oil 75, 95% ale. 25 

 method: [paraffin sections of F 3700 or F 5000 fixed material] — > A, few mins. — > 95% 



ale, wash —* B, 3 days — * wash -^ C, 12 hrs. — * acetone, wash — > toluene, few mins. 



-* clove oil, till toluene removed -^ D, till differentiated -^ balsam, via toluene 



21.22 Bauer 1941 1799, 114:71 



reagents required: A. 5% ferric alum; B. DS 11.124 Held (1937) 10, water 100; C. 



ADS 21.1 Weigert 1885; D. 5% sodium phosphate, dibasic 

 method: [paraffin sections of F 3700.0010 Zenker 1894 fixed material] — * water -^ A, 20 



mins., 40°C. -^ rinse -^ B, 12 hrs., 40°C. — > rinse -^ C, till differentiated -^ D, wash 



— > wash — > balsam, via usual reagents 



21.22 Benda test. 1933 Cajal and de Castro Cajal and de Castro 1933, 241 



reagents required: A. 10% nitric acid, 5. 2% potassium dichromate; C. 1% chromic 



acid; D. 4% ferric alum; E. "amber yellow" solution of alizarin red S; E. 0.1% tolui- 



dine blue; F. 1% acetic acid; G. creosote 

 method: [pieces of 5 mm. side] — > 95% ale, 2 days or longer -^ A, 24 hrs. — * wash — > B, 



24 hrs. -♦ C, 24 hrs. -^ wash — > [paraffin sections] —* water -^ D, 24 hrs. -+ rinse — > 



E, 2 hrs. — > rinse — > blot — » E, warmed to steaming, 15 mins. —* F, wash -^ abs. ale, 



till dehydrated — * G, till differentiated — > balsam, via xylene 

 recommended for: macroglia. 



