DS 21.22 DYE STAINS OF SPECIAL APPLICATION 413 



21.22 Beyer 1940 591b, 4:65 



REAGENTS REQUIRED : A. F 7000.0000 Miiller 1859; B. DS 11.121 Hansen 1905; C. 2% 

 hydrochloric acid, in 70% ale; D. 0.1% ammonia; E. water 100, acetic acid 0.3, 

 ponceau 2R 0.07, acid fuchsin 0.03; F. water 100, phosphotungstic acid 3, orange G. 2; 

 G 0.5% anilin blue 



method: [5 M sections of formaldehyde-fixed material] — > A, 3-12 hrs. -^ thorough wash 

 — > fi, 5 mins. — > wash -^ C, till differentiated — > wash — > D, till blue — + wash — » jE, 5 

 mins. -^ wash —* F, 5 mins. — > quick rinse — > G, 10-15 mins. -^ abs. ale, least possi- 

 ble time —>■ balsam, via xylene 



RECOMMENDED FOR: astrocytes. 



21.22 Brand 1941 see DS 21.22 Holzer 1921 (note) 



21.22 Eisath 1911 14370, 20:3 



REAGENTS REQUIRED: A. 2% formaldehyde; B. 0.2% mercuric chloride; C. water 70, 



DS 11.124 Mallory 1891 30; D. ADS 21.1 Eisath 1911 

 method: [sections by freezing technique of F 7000.1000 Eisath 1911 fixed material] —> A, 



till required -^ B, 30 sees. — > wash -^ C, on slide, few minutes — > wash — > D, till 



differentiated — * wash — > balsam, via usual reagents 



21.22 Galescu 1908 6630,65:429 



reagents required: A. 6% mercuric chloride; B. F 1360.0010 Galescu 1908; C. acetone 

 100, ADS 12.2 Lugol 1; D. water 80, 95% ale. 20, crystal violet 5, oxalic acid 0.2; E. 

 ADS 12.2 Lugol; F. xylene 50, aniline 50 

 method: [pieces of brain] -^ A, 5 hrs. -> B, 12 hrs. 37°C. -^ B (fresh sol.), 12 hrs. 37°C. 

 -^ B (fresh sol.), 24 hrs. 37°C. -^ wash -^ C, 24 hrs. ^ [3 m paraffin sections, via ace- 

 tone] — * acetone — » D, 10 mins. -^ D, (warmed till steaming) 5 mins. — > E (poured 

 on slide), few moments — > blot -^ F, till differentiated -^ balsam 

 note: a detailed description of the use of this technique is given under DS 21.20 

 above. 



21.22 Hadjioloff 1929 6630, 102:789 



REAGENTS REQUIRED: A. sat. alc. sol. {circ. 0.2%) acid fuchsin; B. sat. aq. sol. {circ. 20%) 



light green 

 method: [5 M paraffin sections of F 1600.0010 Flemming 1884 fixed material]—* water 

 — » A, 5-15 mins., 60°C. -^ wash -h> B, 10-20 mins. — » wash -^ balsam, via usual 

 reagents 



21.22 Held 1909 test. 1933 Cajal and de Castro Cajal and de Castro 1933, 245 



reagents required: A. 1% sodium hydroxide in 80% ale; B. 5% ferric alum; C. to 100 

 1% hematoxylin add an excess of molybdic acid: dilute to an "intense violet" color 

 for use; D. DS 12.221 van Giesen 1896 

 method: [celloidin sections of material fixed in F 3700.1010 Held 1909] —* A, 5 mins. — > 

 wash —> B, 1 min. — » wash — > C, 24 hrs., 50°C. — > rinse -^ B, till connective tissues 

 decolorized — > wash — > D, 15 sees. — > abs. ale, till no more color comes away — » bal- 

 sam, via usual reagents 

 recommended for: macroglia. 



21.22 Holzer 1921 23430, 60 :354 



reagents required: A. water 30, 95% alc. 60, phosphomolybdic acid 0.2; B. chloroform 



80, abs. alc. 20; C. chloroform 80, abs. alc. 20, crystal violet 5; D. 10% potassium 



bromide; E. choroform 60, aniline 40, hydrochloric acid 0.3 

 method: [sections by freezing technique of formaldehyde-fixed material]-^ A, 30-90 



sees. — » float to slide, blot with paper soaked in JB — > C, on slide, 3-5 mins. — > D, wash 



— > blot with filter paper soaked in E -^ E, till differentiated — > balsam, via xylene 

 note: Holzer 1921 (23632, 37:508) differs only in substituting aniline for E above. 



Brand 1912 (23430, 172:531) recommends counterstaining in his DS 12.221. 



21.22 Jakob 1913 test. 1937 Gatenby and Painter Gatenby and Painter 1937, 542 



reagents required: A. ADS 12.1 Weigert 1891, 90, 40% formaldehyde 10; B. 0.1% 

 acid fuchsin; C. sat. aq. sol. {cite. 60%) phosphomolybdic acid; D. DS 13.41 Mallory 

 1901, sol. C 



