414 METHODS AND FORMULAS DS 21.22 



method: [frozen sections of material fixed in A] — > water -^ B, 3-10 mins. — > wash — > 

 C, 1-24 hrs. -^ rinse -* Z), 30 mins. —> 95% ale, till differentiated -* balsam, via 

 usual reagents 



21.22 Lehrmitte and Guccione 1909 20080, 19:205 



REAGENTS REQUIRED: A. 7% mercuric chloride; B. F 1600.0010 Lehrmitte and Guccione 



1909; C. 1.5% Victoria blue; D. ADS 12.2 Lugol; E. aniline 50, xylene 50 

 method: [sections of formaldehyde-fixed material by freezing technique] -^ A, 2 hrs. — > 



B, 2 days -^ wash —* strand section on slide -^ C, poured on slide, warmed to steam- 

 ing -^ drain — * Z), 1 min. -^ drain -^ E, till differentiated -^ balsam, via xylene 



21.22 Mallory 1938 Mallory 1938, 241 



REAGENTS REQUIRED: A. sat. sol. lead chloride; B. 5% oxalic acid; C. DS 11.124 Mallory 

 1900 



method: [2-3 mm. slices of formaldehyde-fixed material] — > ^, 6 wks. -^ wash — > [cel- 

 loidin sections] —* B, 30-60 mins. -^ rinse — > 95% ale. -^ balsam, via usual reagents 



21.22 Merzbacher 1909 11392,12:1 



reagents required: A. 95% ale. 70, water 30, sodium hydroxide 2; B. sat. aq. sol. Vic- 

 toria blue; C. ADS 12.2 Lugol; (1905); D. aniline 50, xylene 50 



method: [10-15 ^ sections] —> A, 2-5 mins. — > rinse ale. ^ water -^ B, 24 hrs.—* C, 

 rinse — > D, till differentiated — > balsam, via x\'lene 



21.22 Meyer 1909 15058, 28:353 



REAGENTS REQUIRED: A. 5% potassiuiu dichromatc; B. ADS 12.1 Weigert 1891 at 37°C.; 



C. DS 11.121 Weigert 1904; D. water 70, ADS 21.1 Weigert 1885 30 



method: [pieces of brain] — > A, till white matter dark brown -^ [50 m sections by celloi- 

 din technique] -^ water -^ B, 24 hrs., 38°C. -^ wash 70% ale. -> C, 24 hrs. -> D, till 

 differentiated — > balsam, via usual reagents 



21.22 Peers 1941 1887a, 32:446 



reagents required: A. Q% mercuric chloride; B. AMS 12.2 Lugol; C. 5% sodium sul- 

 fite; D. 0.025% potassium permanganate; E. 5% oxalic acid; F. DS 11.124 Mallory 

 1900 

 method: [sections of formaldehyde-fixed material] -* .4, 3 hrs., 57°C. — > rinse -^ B, 5 

 mins. — > rinse — > C, 5 mins. rinse —* D, 5 mins. —>■ rinse — > £", 5 mins. -^ thorough 

 wash — » F, overnight — > wash — » balsam, via usual reagents 



21.22 Potter 1910 23632,27:238 



reagents required: A. ADS 12.1 Weigert 1891; B. DS 21.22 Weigert 1903; C. 0.5% 



potassium permanganate; D. ADS 21.1 Weigert 1885 

 method: [15 mm. slices of formaldehyde-fixed material] -^ A, 14 days -^ ale. wash — > 

 [15 M celloidin sections] — » water -* B, 2-3 hrs. —> wash -^ C, till differentiation well 

 started —> D, till differentiation completed —> balsam, via usual reagents 

 recommended for: macroglia. 



21.22 Rubaschkin 1904 1780,63:577 



reagents required: A. sat. aq. sol. (circ. 3%) methyl violet; B. ADS 12.2 Gram 1884; 



C. aniline 

 method: [celloidin sections of F 4700.0010 Rubaschkin 1904 fixed material]^ A, on 



slide, 6-12 hrs.—* B, on slide, ^2 to 1 min. — > C, till differentiated —> balsam, via 



xylene 



21.22 Sokolansky 1935 23632, 51 :378 



reagents required: A. 6% potassium dichromate; B. DS 11.113 Kultschitskv 1889; 



C. F 7000.0000 Mliller 1850; D. ADS 21.1 Pal 1887 (A and B sols.) 

 method: [5-8 m frozen sections] -^ A, 24 hrs., 37°C. -^ rinse -^ B, 4-6 hrs., 50-60°C. -> 



C, 2-3 mins. -^ rinse -^ D {A sol.), few mins. -^ rinse —^D(B sol.), till decolorized — * 



[repeat D (A) — > D {B) cycle if insufficiently differentiated] —> balsam, via usual 



reagents 



