DS 21. 22-DS 21.23 DYE STAINS OF SPECIAL APPLICATION 415 



21.22 Weigert 1891 see DS 21.22 Weigert 1894 (note) 



21.22 Weigert 1894 7276, 17:1184 



REAGENTS REQi;iUEu: A. 5% potassiiuu (lichromate; B. ADS 12.1 Weigert 1891; C. 



water 46, 95% ale. 64, sat. ale. sol. lithium earbonate 4, hematoxylin 1; D. ADS 21.1 



Weigert 1885 

 PREPARATION OF c: Add tlic dye dissolved in ale. to the water and alkali. 

 method: [pieces of brain] — > A, till white matter dark brown (some wks.) — ♦ [embed in 



celloidin] -^ B, 24 hrs., 55°C. -^> [section, 15-20 n] -^ water -^ C, 4-24 hrs. -* wash, 



90% ale. — > wash -^ D, till differentiated -^• balsam, via usual reagents 

 recommended for: neuroglia. 

 note: Weigert 1891 (727(1. 17:1184) omits 1). Weigert 1903 (Khrlich, Krause, etal. 1910, 



942) substitutes for C the following: To 50 0.4% ferric chloride add 50 1 % hematoxylin 



in 95% ale. 



21.22 Weigert 1895 lest. 1937 Gatenby and Painter Gatenby and Painter 1937, 337 



This method involved the use of a proprietary reagent of secret composition and cannot 

 therefore be further noticed. Any of the Victoria blue or crystal violet methods re- 

 corded in this section may be substituted. 



21.22 Weigert 1903 see DS 21.22 Weigert 1894 (note) 



21.22 Windle, Rhines, and Rankin 1943 see DS 22.3 Windle, el al. 1943 (note) 



21.23 METHODS FOR OTHER NERVOUS ELEMENTS 



21.23 Koinikow test. 1933 Cajal and de Castro Cajal and de Castro 1933, 320 

 reagents required: .4. F 7000.1000 Orth 1896; B. F 7000.0000 Miiller 1859; C. F 1700.- 



0000 Marchi 1886; D. sat. aq. sol. phosphomolybdic acid; E. DS 13.7 Mann 1894a 

 {A sol.); F. 0.1% sodium hydroxide in abs. ale; G. 0.01% acetic acid in abs. ale. 



method: [piece of nerve] —> A, 24 hrs. ^ B, till required—* C, 8-10 days — > [teased 

 preparations or celloidin sections] —* D, 1 hr. -* wash — » E, 24 hrs. — > rinse -* abs. 

 ale, till dehydrated -^ F, till color changes from blue to red -^ wash, abs. ale. — > G, 

 till color changes from red to blue -^ wash, abs. ale. -^ xylene — * white petroleum or 

 "neutral mountant" 



recommended for: Schwann cells. 



21.23 Fieandt test. 1933 Cajal and de Castro Cajal and de Castro 1933, 265 



reagents required: A. 10% iodine in 95% ale; B. 0.25% sodium thiosulfate; C. DS 



11.124 Mallory 1900; D. 10% ferric chloride in abs. ale. 

 method: [5 M sections of 2 mm. slices fixed in F 3000.0012 Heidenhain 1909] — > water 



-^ A, few mins. — * 95% ale. till no more color comes away -^ B, 1 hr. —> wash — > C, 



10-14 hrs. — > blot -^ D, till differentiated, some hrs. — » blot —> quick rinse — > abs. 



ale., 24 hrs. — > balsam, via oil of thyme 

 recommended for: gliosomes. 



21.23 Nageotte test. 1933 Cajal and de Castro Cajal and de Castro 1933, 325 



reagents required: A. 30% ale; B. 0.1% nitric acid; C. DS 11.122 Cajal and de 



Castro 1933; D. DS 12.211 Cajal 1895 

 method: [fresh piece of nerve] —* A, 1 day — > B, till fibers dissociated -^ [tease on slide] 



—y C, till deep blue -^ wash — » D, till differentiated —^ balsam, via usual reagents 

 recommended for: syncytium of Schwann cells. 



21.23 Nageotte test. 1933 Cajal and de Castro Cajal and de Castro 1933, 319 



reagents required: A. 2.5% ferric alum; B. 1% hematoxylin 



method: [pieces of nerve, fixed in F 3900.1000 Dominici 1905 and teased on a slide] 

 —y A, some hrs. — > B, some hrs. —> C, till differentiated — > wash —* balsam, via usual 

 reagents 

 recommended for: selective staining of Schwann cells. 



21.23 Reich see DS 22.8 Reich (1933) 



