DS 21.3 DYE STAINS OF SPECIAL APPLICATION 417 



21.3 Cunningham 1920 1845, 26:405 



REAGENTS REQUIRED: A. 0.3% brilliant cresyl blue in 95% ale; B. DS 13.12 Wright 1910 

 method: [dip coverslip in A and dry] — > make smear on clean cover -^ press to A — » 



separate — ♦ dry — > B, full technique —> dry 

 recommended for: reticulocytes. 



21.3 Dekhuyzen 1901 766, 19:536 



formula: water 100, osmic acid 1.8, acetic acid 0.6, methylene blue 0.1 

 METHOD : blood drop on slide -^ stain, mixed with drop — > smear — > dry 



21.3 Doherty, Suk and Alexander 1938 1879, 40:158 



REAGENTS REQUIRED! .1. Water 50, abs. ale. 50, benzidine 0.5, sodium nitroferricyanide 

 0.1; B. water 50, abs. ale. 50, acetic acid 2, hydrogen peroxide 0.5, sodium nitroferri- 

 cyanide 0.1 



PREPARATION OF REAGENT A: Dissolvc the benzidine in the abs. ale. Dissolve the nitro- 

 ferricyanide in 10 water. Mix and dilute to 100. 



method; [200-300 m sections of formaldehyde fixed material]—* wash — > A, with fre- 

 quent agitation 10 mins. — > rinse — > 5, with frequent agitation, till capillary net 

 stained — * wash -^ balsam, via usual reagents 



RECOMMENDED FOR: differential staining of blood in capillaries in sections. 



21.3 Dubuscq 1899 1915, 6:481 



formula: water 250, copper chloride 0.6, copper acetate 0.6, osmic acid 0.6, acetic acid 



0.6, thionin 0.6 

 method: Mix equal parts stain and arthropod blood. Leave 5 mins. Make smear. 



21.3 Ellermaner 1919 23633, 36:56 



REAGENTS REQUIRED: A. Water 100, eosin B 1, neutralized formaldehyde 5; B. DS 13.13 



Maximow 1924 

 method: [5 n sections of F 3700.1000 Maximow 1909 material] -* water — > drain — » A, 

 15 mins. — > wash, 2 mins., 45°C. — > B, 30 mins. -^ wash -^ blot — > abs. ale, till sec- 

 tion blue -^ balsam, via xylene 



21.3 Epstein test. 1946 Roskin Roskin 1946, 243 



REAGENTS REQUIRED: A. water 100, citric acid 1, toluidine blue 1; B. sat. aq. sol. {circ. 



1%) picric acid 

 method: [dried smears] — ^ A, 20-30 mins. -^ rinse -^ B, 1-2 sees. — > wash -^ dry 



21.3 Fiessinger and Laur 1930 test. 1942 Langeron Langeron 1942, 1023 



formula: sat. ale. sol. cresyl blue 75, sat. ale. sol. (circ. 2.5%) neutral red 25 

 METHOD : Mix equal parts blood and stain — > smear — ♦ dry 

 note: Recommended for reticulocytes. 



21.3 Flinn 1939 see DS 23.219 Flinn 1939 



21.3 Freifeld 1931 test. 1950 Jones Jones 1950, 225 



formula: water 100, DS 11.43 Ziehl 1882 1, 1% methylene blue 0.7 

 method: [methanol-fixed smears] —* stain, 1 hr. -^ dry 

 RECOMMENDED FOR: toxic neutrophiles. 



21.3 Graham 1916 11343,35:231 



REAGENTS REQUIRED: A. 95% alc. 90, 40; formaldehyde 10; B. water 60, 95% ale. 40, 



a-naphthol 1, hydrogen peroxide 0.2; C. water 60, 95% alc. 40, pyronin 0.1, aniline 4; 



D. 0.5% methylene blue 

 method: [air-dried smears]-^ A, 1-2 mins. — > wash — > B, 4-5 mins. — > wash — > C, 2 



mins. — > wash — * D, 1-2 to 1 min. — » wash — > blot — > dry 

 recommended for: demonstration of oxidase granules. 



21.3 Graham 1918 11313, 39:15 



REAGENTS REQUIRED: A. 95% all;. 90, 40*;;; fornialdohydc 10; B. water 00, 95 7o alc. 40, 



hydrogen peroxide 0.2, benzidine q.s. to sat. immediately before use; C. DS 11.44 



Loffler 1890 

 method: [air dried smears] -^ A, 1-2 mins. -♦ wash — ♦ B, 5-10 mins. — » wash — > C, 3 



sees. — > wash — > blot -^ dry 

 RECOMMENDED FOR: demonstration of oxidase granules. 



