418 METHODS AND FORMULAS DS 21.3 



21.3 Groat 1936 see DS 13.13 Groat 1936 



21.3 Grosso 1914 16059, 6:235 



PREPARATION OF DRY STOCK: Add a sat. sol. picric acid to a sat. sol. methylene blue until 



no further ppt. forms. Filter. Wash and dry ppt. 

 WORKING solution: methanol 100, dry stock 0.5 

 method: [dry smear] —* 10 drops, placed on slide, 3-4 mins. -^ 10 drops water, added to 



stain on slide, 5 mins. —>■ wash — » dry 



21.3 Hayem 1889 23632, 6 :335 



formula: water 100, mercuric chloride 0.25, sodium sulfate 2.5, sodium chloride 0,5. 



eosin 0.05 

 method: Mix 1 blood with 100 stain. Make smear, 



21.3 Kahlden and Laurent 1896 Kahlden and Laurent 1896, 134 



formula: sat. aq. sol. orange G 25, sat. 20% ale. sol. acid fuchsin 25, 95% ale. 15, sat. 



aq. sol. methyl green 25 

 preparation: Mix in order given. Leave 24 hours. Decant supernatant stain (do not 



filter). 

 method: [dry smear] -^ stain 5-10 mins. — > wash —* dry — > balsam 



21.3 Kardos 1911 8545, 12 :39 



preparation of dry stain: Mix equal parts 2% orange G and sat. sol. methyl blue. 



Filter. Wash and dry ppt. 

 reagents required: A. DS 13.11 May-Griinwald 1902; B. water 95, DS 13.13 Giemsa 



1902 3, sat. sol. above ppt. in methanol 2 

 method: [smear] —* A, 3 mins. on slide —* add water to A on slide, 1 min. -^ drain -^ B, 



on slide, 15 mins. — > rinse -^ dry 



21.3 Kuhn 1933 see DS 13.13 Kuhn 1933 



21.3 Liebmann 1942 20540b, 17:31 



REAGENTS REQUIRED: A. DS 13.12 Wright 1910; B. 0.01% acetic acid 

 method: [smears fixed in F 1700.1000 Maximow 1909] -^ A, 24 hrs. —> B, 1-1,' 2 mins. -> 



balsam, via acetone and xylene 

 recommended for: invertebrate blood. 



21.3 Liebmann 1945 20540b, 20 :83 



REAGENTS REQUIRED: A. water 100 azur II 0.005, eosin Y 0.0075 

 method: [fix smears 10 mins. in formaldehyde vapor: flame 5 times] — > A, 6 hrs. -^95% 



ale, 1-2 mins. — ^ abs. ale, 30-60 sees. -^ balsam, via xylene 

 RECOMMENDED FOR: amphibian blood. 



21.3 Lightwood, Hawksley and Bailey 1935 16916, 28:405 



formula: abs. ale. 100, nevitral red 0.04, Janus green B 0.3 

 method: Dip clean slides in stain. Let dry. Make smear on .slide. 



21.3 McCullough and Dick 1942 see DS 23.219 McCullough and Dick 1942 



21.3 Michaelis 1899 see DS 13.11 Michaelis 1899 



21.3 Moore 1882 test. 1884 Cole Cole 1884, vol. 1, 12 



REAGENTS REQUIRED: A. water 50, 95% ale. 50, eosin W 1; B. 0.3% methyl green 

 method: [dried smear of amphibian blood] —> A, on slide, 2 mins. -^ rinse -^ B, on slide, 

 2 mins. — > wash — ♦ dry —* balsam 



21.3 Okajima 1917 763, 11 :295 



REAGENTS REQUIRED: A. 10% phosphomolybdic acid; B. water 100, alizarin red S 6, 



phosphomolybdic acid 2.5 

 method: [sections of material (not picric-fixed)]—* A, 1 niin. -^ water, quick rinse—* 



stain, 1-24 hrs. — > Ijalsam, via usual reagents 

 result: erythrocytes, orange yellow; other tissvies, colorless. 

 recommended for: specific staining of erythrocytes in sections. 



