DS21.3 DYE STAINS OF SPECIAL APPLICATION 419 



21.3 OUver 1934 see DS 22.8 Oliver 1934 



21.3 Osgood and Wilhelm 1934 1128-4, 19:1129 



REAGENTS REQUIRED: A. 1% brilliant cresyl blue in 0.85% sodium chloride 

 method: Mix 5 ml. each A and oxalated blood. Prepare and dry smear. 

 RECOMMENDED FOR: reticulocytes. 



21.3 Pappenheim 1917 8545, 22:15 



REAGENTS REQUIRED: A. US 13.11 May and Grlinwald 1902; B. DS 13.13 Slider and 



Downey (1929) 

 method: [dry smears] —> A,3 mins. on slide — » add equal vol. water to A on slide, 1 min. 



—y drain — » B, on slide, 15 to 30 mins. — » water till differentiated, al:»out 1 min. — > dry 



21.3 Pickworth 1934 11025,69:02 



REAGENTS REQUIRED: A. watcr 75, acetic acid 0.5, benzidine 0.125, sodium nitroprusside 



0.1; B. water 100, hydrogen peroxide (3%) 0.5 

 method: [250 m sections of formaldehyde-fixed brain, sectioned by E 11.1 Pickworth 



1934] — ♦ thorough wash — > A, 1 hr., 37°C. with continuous shaking — > wash — > 



balsam, via usual reagents 

 recommended for: blood vessels in thick sections of brain. 



21.3 Price-Jones 1933 test. 1937 Gatenby and Painter Gatenby and Painter 1937, 397 

 formula: water 90, sodium chloride 0.7, 40% formaldehyde 10, gentian violet 0.01 

 use: Use as blood diluent. Make smear. 



21.3 Pryce 1939 11284,49:594 



reagents required: A. sat. sol. brilliant cresyl blue in abs. ale; B. DS 13.12 Leishman 



1901 

 method: [dip slides in A and air dry. Make smear on coated slide and prevent evapora- 

 tion by laying another slide not quite in contact with smear. Leave 5 mins. Remove 

 cover. Dry] — ♦ B, on slide, 5 mins. — > dilute stain on slide, leave 5 mins. — > wash till 

 smear changes from blue to red -^ dry 

 recommended for: demonstration of reticulocytes. 



21.3 Pugsley 1940 test. 1944 Randall 20540b, 19:150 



reagents required: A. water 100, potassium oxalate 0.2; brilliant cresyl blue 1; B. 



anj^ DS 13.12 mixture 

 method: [mix equal parts A and blood, leave 1 min.] — » make smear — > B, till counter- 

 stained 

 recommended for: reticulocytes. 



21.3 Raadt 1912 see DS 13.12 Raadt 1912 



21.3 Rossi 1889 23632, 6 :475 



formula: water 100, osmic acid 0.5, methyl green 5 



method: [blood, drop on slide] -^ stain, mixed with drop -^ make smear —y dry — > glyc- 

 erol, via graded glycerol water mixtures 



21.3 Sabin 1923 10910,34:277 



formula: abs. ale. 100, neutral red 0.1, Janus green 0.05 

 method: Dip clean slide in stain. Let dry. Make smear on slide. 



21.3 Sato 1928 11284, 13:1058 



reagents required: A. 0.5% copper sulfate; B. benzidine 0.1, water 100, hydrogen 



peroxide 0.2; C. 1% safranin 

 method: [dry smears on coverslips] -^ A, floated on solution, 1 min. — » rinse -^ B, 2 



mins. — > wash — > C, 20 sees. -^ wash —* blot -^ dry 

 recommended for: demonstration of peroxidase granules. 

 result: peroxidase granules, blue; basophilic granules and nuclei, red. 



21.3 Saye 1943 Tech. Bull., 4:12 



reagents required: A. 0.25%, eosin Y in 95% ale, 0.05% thionine in 80% ale. 

 method: [air-dried smear] — » A, 1 min. — > drain briefly —yB,l min. — » wash — + dry 



