420 METHODS AND FORMULAS DS 21.3 



21.3 Schmorl 1928 see DS 22.8 Sclimorl 1928 



21.3 Sheehan 1939 11431,49:58 



REAGENTS REQUIRED: A. sat. sol. Sudan black B; B. sat. sol. ethyl eosin in 70% ale; 



C. sat. sol. methylene blue 

 method: [methanol-fixed smear] -^ A, 30 sees. -* wash — » 70% ale, Imin.-^ B, 30 sees. 



— * wash — >• C, 3 mins. -^ rinse -^ blot — > dry 

 recommended for: lipoid granules in leukocytes. 



21.3 Sheehan and Storey 1947 11431, 59:336 



stock solutions: I. 0.3%, Sudan black B in abs. ale; II. water 100, abs. ale. 30, phenol 



16, sodium phosphate, dibasic (cryst.) 0.3 

 preparation of b: Add the phenol dissolved in ale. to the phosphate dissolved in water. 

 reagents required: A. stock I 60, stock II 40; B. DS 13.13 Giemsa 1902 1, water 100; 



C. 0.2% potassium dihydrogen phosphate 

 method: [smear fixed in formaldehyde vapor] -^ A, 10-60 mins. -^ 70% ale., rinse— » 



wash -^ B, 5 mins. — > C, till differentiated -^ wash dry 

 recommended for: fat granules in leukocytes. 



21.3 Simpson 1922 11343, 40:77 



formula: 95% ale. 100, neutral red 1, Janus green 0.5 

 method: Dip clean slide in stain. Leave dry. Make smear on slide. 



21.3 Slominski and Cunge test. 1948 Romeis Romeis 1948, 470 



preparation of stain: To 100 1% benzidine add 10 of 10% hydrogen peroxide (3%). 

 method: [120 m frozen sections of material fixed in F 8000.1000 Slominski and Cunge 



(1948)] — > water -^ A, till sufficiently stained — > balsam, via usual reagents 

 recommended for: differential staining of capillaries in sections. 



21.3 Steil 1936 20540b, 11 :99 



reagents required: A. DS 13.12 Wright 1910 



method: [dry smears] —* A, on slide, 1-2 mins. — >■ equal amount water added to stain, 

 3 mins. — > drain — » flood with water till smear pink -^ drain -^ methanol, 1-2 mins. 

 -^ water, till smear pink -^ abs. ale. —> balsam, via clove oil 



21.3 Strumia 1936 11284,21:930 



preparation of stock solutions: I. DS 13.13 Giemsa 1902 (dry powder) 0.2, glycerol 



12, methanol acetone 44; II. DS 13.11 May-Grtinwald 1902 (dry powder) 0.04, 



methanol 50, acetone 50. 

 REAGENTS REQUIRED: A. stock I 65, stock II 35; B. 0.2% sodium carbonate 

 method: [air-dried smear] — * 1 ml. A, on slide, 2 mins. -^ add 1 ml. B, leave 3 mins. -^ 



wash — > dry 



21.3 Thompson 1945 1887a, 38:49 



REAGENTS REQUIRED: A. 4% forric alum ; B. sat. sol. picric acid 87, 1% acid fuchsin 13 

 method: [sections or smears, with nuclei prior stained in DS 11.122 Mallory 1938]^ 



wash-^ A, 1 min. —* rinse -^ B, 15 mins. — > 95% ale., till differentiated -^ balsam, 



via usual reagents 

 result: structures or pieces containing hemoglobin, green; other structures, yellow, 



red, brown, and gray. 

 RECOMMENDED FOR: differentiation of erythrocytes, and pieces of erythrocytes, in 



smears and sections. 



21.3 Ugruimow 1928 23632, 45:191 



REAGENTS REQUIRED: 0.1% azur II in phosphate buffer pH 6.3 15, 0.1% eosin BA in 



phosphate buffer pH 6.3 16, water 100 

 method: [heat-fixed smear] —> stain, 10-12 hrs. ^ acetone, till differentiated —» acid 

 balsam 



21.3 Westphal test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 134 



formula: a DS 11.21 Grenacher 1879 30, sat. ale. sol. crystal violet 30, glycerol 30, 



phenol 25, acetic acid 5 

 method: [dry smear] -^ stain, 20-30 mins. — » wash — > balsam, via usual reagents 



