DS 21.3-DS 21.411 DYE STAINS OF SPECIAL APPLICATION 421 



21.3 Willebrand 1901 see DS 13.11 Willcbrand 1901 



21.3 Ziegler 1945 test. 1945 Riley 20504b, 21 :37 



REAGENTS REQUiREu: A. Water 50, abs. ale. 50, benzidine 0.5, sodium nitroprusside 0.1; 

 B. water 47, abs. ale. 50, acetic acid 3, hydrogen peroxide (30%) 0.5, sodium nitro- 

 prusside 0.1; C. 2% acetic acid in 70% ale; D. 2% acetic acid in 90% ale. 

 method: [formaldehyde-ti.xed cornea] -^ thorough wash— ♦A, 20 mins. -^ brief wash 

 — > B, 15 mins. -^ B, fresh solution, 45 mins., 37°C. wash — > C, 10 mins. — > D, 10 

 mins. — > abs. ale. — > M 23.1 mountant 

 RECOMMENDED FOR: demonstration of capillaries in wholemounts of cornea. 



21.4 Special Stains for Other Tissues 



21.41 designed to differentiate types of tissues not covered under 21.1, 21.2 



OR 21.3 



21411 Plant Tissues 



21.411 Buchholz 1931 20540b, 6:13 



REAGENTS REQUIRED: A. 1% acid fuchsin 80, 1%, Hght green in 90% ale. 20; B. 80% 



lactic acid 

 method: [cortex dissected from pistil killed in formalin-alcohol] —* A, Q hrs. —* rinse -* 



B, till cleared and differentiated — > [seal in B under coverslip] 

 RECOMMENDED FOR: Staining wholemounts to show pollen tubes. 



21.411 Chandler 1931 20540b, 6:25 



REAGENTS REQUIRED: A. DS 11.23 Schneider 1880; B. sat. aq. sol. {circ. 0.3%) magenta 

 method: [cortex dissected from pistil killed in formalin-alcohol]—* A, on slide, few 

 sees. — > B, dropped on A on slide, 1 drop, few sees. -^ bolt -^ dehydrate by drop 

 method —>■ balsam, via xylene 

 RECOMMENDED FOR: staining wholemounts to show pollen tubes. 



21.411 Gardiner 1888 Arb. hot. inst. Wiirz, 3:52 



REAGENTS REQUIRED: A. 50% sulfuric acid; B. water 50, 95% ale. 50, picric acid to sat., 



anilin blue 1 

 method: [sections] —>• water —» a, 2-10 sees. -^ wash —> B, 10 mins. -^ wash, till no 



more color comes away —y glycerol 

 recommended for: demonstration of protoplasmic connections between plant cells. 



21.411 Johansen 1939 20540b, 14:125 



REAGENTS required: A. DS 11.42 Johansen 1940; B. 1% methyl violet 2B; C. 95% ale. 



30, methyl cellosolve 30, tert. butyl ale. 30; D. sat. sol. fast green FCF in equal parts 



clove oil and methyl cellosolve 15, 95% ale. 40, tert. butyl ale. 40, acetic acid 1.5; E. 



DS 12.16 Johansen 1940; F. clove oil 30, abs. ale. 30, methyl cellosolve 30 

 method: [sections] — > 70% ale. -^ A, 1-2 days —>■ rinse -^ B, 10-15 mins. — > rinse -^ C, 



5 sees. — » D, 10 mins. —* C, 5 sees. -^ E, 5 mins. — > F, rinse — > balsam, via usual 



reagents 

 recommended for: plant histology and cytology. 



21.411 Johansen 1940 Johansen 1940, 93 



REAGENTS REQUIRED: A. 1% acid fuchsin in 70% ale.; B. DS 12.16 Johansen 1940 fast 



green; C. DS 13.5 Johansen 1940 (solution H) 

 method: [sections] — * 95% ale. — * A, 20 mins. -^ water, rinse — » 95% ale, rinse -^ B, 



on slide, few sees. -^ C, till clear -^ balsam, via xylene 

 recommended for: differentiation of reproductive structures in marine Phaeophyta. 



21.411 Meyer test. 1915 Chamberlain Chamberlain 1915, 130 



REAGENTS REQUIRED: A. ADS 12.2 Gram 1884; B. water 75, sulfuric acid 25, iodine to 



sat. ; C. 3 % methyl violet 

 method: [sections] —» water —» yl, 3-5 mins. — ♦ 4, under coverslip -^ 5, run under 



coverslip -^ C, run under coverslip, 3 mins. — >• wash — > glycerol 

 recommended for: demonstration of protoplasmic connections between plant cells. 



