422 METHODS AND FORMULAS DS 21.411-DS 21,412 



21.411 Nebell931 20540b, 6:27 



REAGENTS REQUIRED: A. water 100, martius yellow 0.05, resorcin blue 0.05, ammonium 



hydroxide q.s. to give pH 8 

 method: [sections of styles, or crushed styles, or cortices dissected from styles fixed 



in F 0000.0010 Carnoy 1887] —+ wash, till acid-free-^ A, 2-5 mins. -^ balsam, via 



graded ales, containing resorcin blue at pH 8 

 RECOMMENDED FOR: staining pollen tubes. 



21.411 Strasburger test. 1915 Chamberlain Chamberlain 1915, 130 



REAGENTS REQUIRED: A. 1% osmic acid; B. ADS 2.2 Gram 1884 60, water 30; C. 25% 



sulfuric acid; D. 25% sulfuric acid 100, iodine to sat., 3% methyl violet 0.1 

 method: [sections of fresh material] —> A, 5 mins. -^ wash -^ B, 20-30 mins. -^ C, 24 



hrs. —^D,5 mins. — * glycerol 

 RECOMMENDED FOR: demonstration of protoplasmic connections between plant cells. 



21.411 Watkin 1925 11211, 15:340 



REAGENTS REQUIRED: A. Water 25, glycerol 25, lactic acid 25, phenol 25, methyl blue 0.1 



method: Fix, stain, and mount style in A. 



RECOMMENDED FOR: demonstration of pollen tubes in flowers of Gramineae. 



21412 Animal Tissues 



21.412 Badertscher 1940 20540b, 15 :29 

 REAGENTS REQUIRED: A. DS 22.4 Hcrxheimer 1901 



method: [thick, freehand sections of formaldehyde-fixed skin] —^70% ale, till formal- 

 dehyde-free -^ A, 12-24 hrs. -* 70% ale. till glands differentiated -> glycerol -> M12 

 mountant (Chapter 26) 



recommended for: differential staining of sebaceous glands in wholemounts. 



21.412 Behrens 1898 23328, 3 :76 



formula: A. ADS 12.2 Merkel (1898); B.\% potassium permanganate; C. 1% sodium 

 sulfite; D. 0.5% formic acid; E. 1% methyl violet 



method: [sections of formaldehyde-fixed material] -^ A, 4 or 5 days — > B, 10 mins. — > 

 wash -^ C, 5 mins. -^ D, few sees. -^ [repeat C, D cycle till bleached] -^ wash — > E, 

 2 mins. — > 95% ale, till no more color comes away -^ balsam, via xylene 



recommended for: bile capillaries. 



21.412 Bloom 1925 see DS 21.412 Eppinger 1902 (note) 



21.412 Buzz! 1898 14352,8:149 



reagents required: A. sat. sol. picric acid; B. \% nigrosin 

 method: [sections of formaldehyde-fixed material] -^ water -^ A, b mins. — ♦ rinse — * B, 



1 min. — » rinse — > 95% ale, minimum possible time -^ balsam, via origanum oil 

 recommended for: differentiation of keratin from eleidin in skin sections. 

 result: eleidin, blue-black; keratin, yellow. 



21.412 Clara 1933 23639b, 17:698 



reagents required: A. ADS 11.1 Clara 1903; B. DS 11.113 Kultschitzky 1889; C. 



ADS 21.1 Weigert 1885 

 method: [celloidin sections of formaldehyde-fixed material]—* water—* A, 1 day, 40- 



50°C. — > rinse -^ iJ, 1 day, 37°C. -^ wash — > C, till differentiated — > wash — > balsam, 



via usual reagents 

 recommended for: differential staining of bile capillaries. 



21.412 Clara 1934 23507a, 35:1 



reagents required: A. water 100, potassium dichromate 3.2, chrome alum 1.6, chromic 



acid 0.125, ammonium molybdate 0.25; B. DS 11.113 Kultschitzky 1889 

 method: [sections of alc.-formaldehyde-fixed material]-^ A, 24 hrs., 50°C. — + rinse —> 



B, till sufficiently stained -^ wash -^ balsam, via usual reagents 

 recommended for: demonstration of bile capillaries. 



