DS 21.412 DYE STAINS OF SPECIAL APPLICATION 423 



21.412 Dahlgren 1929 McClung 1929, 306 



REAGENTS REQUIRED: A. sat. sol. picric acid 100, nigrosin 2 

 method: [sections fixed, or mordanted, in picric acid] -^^ waters A, 5 mins. — > 95% 



ale, till color clouds cease — > balsam, via usual reagents 

 RECOMMENDED FOR: to distinguish muscle from other connective tissues. 

 result: muscle, yellow; other connective tissues, brown. 



21.412 Dublin 1944 see MS 32.1 Dublin 1944 



21.412 de Galantha test. 1934 Wilbur 1887a, 18:157 



reagents required: A. F 3700.0010 Zenker 1894; B. ADS 12.2 Lugol; C. 2% sodium 

 thiosulfate; D. ADS 12.1 Weigert 1896; E. ADS 12.1 Wcigert 1891; /'\ 1% azocarmine 

 in 1% acetic acid; G. 1% aniline in 95% ale; H. 1% hydrochloric acid in 95% ale; /. 

 5% phosphotungstic acid; /. water 100, acetic acid 8, orange G 2, anilin blue 0.5 

 method: [paraffin sections of formaldehyde-fixed material] — * water — > A, 1 hr. —> wash 

 -^ B, 5 mins. — > wash — ^ C, till bleached —> wash — > Z), 4 hrs. —y wash —* E, 2 hrs. —>■ 

 running water, 1 hr. — » F, 40 mins. —y G, till nuclei differentiated — » H, wash —^ rinse 

 —> I, 2 hrs. -^ rinse — > /, 5 mins. — > rinse -^ abs. ale, till differentiated — > balsam, 

 via xylene 

 RECOMMENDED FOR: histological differentiation of kidney. 



21.412 Eppinger 1902 2526,31:230 



RE.'i.GENTs required: A. 4% formaldehyde; B. ADS 12.1 Weigert 1891; C. 1% hema- 

 toxylin; D. 5% cupric acetate; E. ADS 21.1 Weigert 1885; F. 1.5% lithium carbonate 



method: [small pieces] — + A, 5-10 days -^ B, 10 days -^ wash — > celloidin sections — > C, 

 24 hrs. -^ rinse — > D, 5 mins. -^ wash — > E, till sections differentiated -^ wash -^ F, 

 till celloidin decolorized — » balsam, via usual reagents 



recommended for: demonstration of bile capillaries in liver. 



note: Bloom 1925 (590, 36 :455) uses B 1 week at 37.5°C. and C for only a few moments. 



21.412 Forsgren 1928 23639b, 6:647 



reagents required: A. 3% barium chloride; B. 4% formaldehyde; C. 0.1% acid 



fuchsin; D. 1% phosphomolybdic acid; E. DS 13.41 Mallory 1900 (C sol.) 

 method: [3-4 mm. slices of liver] — > A, 6-12 hrs. -^ B, 12-18 hrs. -^^ 95% ale, quick 



wash — > [5 M paraffin sections] — > water -^ C, 1-3 mins. — > wash — * D, y^-l min. — > 



rinse -^ E, 3-5 mins. -^ wash — > balsam, via usual reagents 

 recommended for: differential staining of bile duct. 



21.412 Hansen 1898 see DS 12.221 Hansen 1898 



21.412 Heidenhain 1903 23632,20:179 



REAGENTS REQUIRED: .4. 1% thiazin red; B. \% thionin 

 method: [sections of trichloroacetic-fixed material]-^ water —> A, 1-6 hrs., till deeply 



stained —> rinse— > B, 1-12 hrs. ^^ 95% ale till differentiated—* balsam, via usual 



reagents 

 recommended for: contraction bands on striped muscle. 



21.412 Holmer 1927 test. 1948 Romeis Romeis 1948, 495 



reagents required: A. 30% ferric chloride; B. 0.5% hematoxjdin; C. 0.1% ferric 



chloride; D. sat. sol. lithium carbonate 

 method: [sections of formaldehyde-fixed material] —^ water ^ A, 3-5 mins. —> short 



wash — > B, 5-10 mins. -^ rinse -^ C, till differentiated — > wash -^ D, till blue -^ balsam, 



via usual reagents 

 recommended for: differential staining of bile capillaries. 



21.412 Kockel 1899 23681, 10:749 



reagents required: A. \% chromic acid; B. DS 21.212 Weigert 1885 (sol. C); C. 10% 



ammonium alum; D. ADS 21.2 Weigert 1885 50, water 50 

 method: [paraffin sections] —> water -+ A, 5-10 mins. — > quick rinse — > B, 15-20 mins. 

 — > wash — > C, till dark blue — > wash -^ D, till differentiated — > wash — > C, till back- 

 ground decolorized -^ countcrstain with any DS 11.21 formula if required -^ balsam, 

 via usual reagents 

 recommended for: fibrin in sections. 



