424 METHODS AND FORMULAS DS 21.412 



21.412 Kramer 1948 19938, 108:141 



REAGENTS REQUIRED: A. 0.5% eosin in 95% ale; B. methyl salicylate 

 method: [Bouin 1897 F 5000.1010 fixed material] -> 50% ale, 10 mins. -^ 70% ale, 

 1 hr. -^ 95%, ale, 10 mins. -^ A, 6-8 hrs. or until specimen uniform pink color -> 95% 

 ale, 30 mins. -^ B, drops added to ale, at such intervals as wUl prevent collapse of 

 specimen — » B 

 RECOMMENDED FOR: differential staining of muscles in insect wholemounts. 

 result: muscles pink; other tissues greenish. 

 NOTE : Times cited are for 4-day-old housefly larvae. 



21.412 Kromayer 1892 1780,39:141 



reagents required: A. sat. sol. methyl violet 6 B. 50, sat. sol. aniline 50; B. ADS 11.1 



Lugol (1905) 30, water 60; C. xylene 60, aniline 30 

 method: [5 M sections] -^ water -^ A, 10-15 mins. -+ thorough wash -^ B, 1-30 sees. 



— > drain -^ blot -^ C, 50°C., till differentiated — > balsam, via xylene 

 recommended for: epithelial fibers in skin. 



21.412 Kultschitzky 1875 1780, 46:675 



reagents required: A. water 100, acetic acid 3, acid fuchsin 0.5; B.2% acetic acid; C. 



water 100, acetic acid 3, anilin blue WS 0.5 

 method: [sections of F 7000.0000 Miiller 1859 material]-^ A, 3-5 mins. -^ B, till no 



more color comes away -^ C, till differentiated -^ balsam, via usual reagents 

 recommended for: reticulum fibers. 



21.412 Lillie 1948 Lillie 1948, 189 



REAGENTS REQUIRED: A. water 100, nitric acid 0.5, sodium periodate 1; B. DS 11.44 



Schiff 1866; C. 0.5% sodium bisulfite; D. DS 11.121 Weigert 1903; E. 1% orange G 

 method: [sections] — » water —y A, 10 mins. -^ wash -^ B, 3-5 mins. — » C, 3 successive 



baths, 1>^ mins. each — > wash — > D, 1-2 mins. — > wash — > E, till plasma sufficiently 



stained — » balsam, via usual reagents 

 recommended for: reticulum fibers. 



21.412 Long 1948 see MS 33.44 Long 1948 



21.412 Mall test. 1950 Hall and Herxheimer Hall and Herxheimer 1950, 83 



REAGENTS REQUIRED: A. water 100, sodium bicarbonate 10, pancreatin 5; B. water 90, 



95% ale. 10, picric acid to sat.; C. water 65, 95% ale 35, acid fuchsin, 10 

 method: [sections by freezing technique] —> A,24: hrs. — > wash, with agitation — * strand 



sections on slide and dry — * B, dropped on slide and allowed to dry — > C, 30 mins. — » 



balsam, via usual reagents 

 recommended for: reticulum fibers. 



21.412 Neubert 1940 23418(1), 110:709 



REAGENTS REQUIRED: A. DS 11.41 Petersen 1926; B. 5% phosphotungstic acid 

 method: [sections] —> water—* A, 5-30 mins. -^ B, till differentiated —» wash -*■ bal- 

 sam, via usual reagents 

 RECOMMENDED FOR: smooth muscle in sections. 



21.412 Pinkus 1944 1829, 49 :355 



REAGENTS REQUIRED: A. Water 30, 95% ale 70, hydrochloric acid 0.6; orcein 1; B. 0.3% 



hydrochloric acid in abs. ale; C. DS 13.13 Giemsa 1902 0.6, water 100; D. 0.001% 



eosin Y in 95% ale 

 method: [sections] —> 70% ale — > A, 1-9-1 hr. —> rinse —>• 95 % ale, few dips -* abs. 



ale, till decolorized to pale brown — > B, till almost completely decolorized — > wash 



— * C, 2-12 hrs. -^ blot — > D, tiU dehydrated — > balsam, via usual reagents 

 recommended for: sections of skin. 



21.412 Unna 1928 test. 1928 Schmorl Schmorl 1928, 343 



reagents required: A. water 100, ammonium alum 10, crystal violet 1.5; B. ADS 12.2 



Lugol (1905) 2, water 98, C. aniline 20, xylene 80; D. aniline 50, xylene 50 

 method: [celloidin sections of ale fixed material] —» water -^ A, 1 hr. — » wash — » B, 



30 sees. — > C, 1^-1 min. — > D, dropped on slide, till differentiated — > xylene — » balsam 

 recommended for: fibers in skin. 



