DS 21.412-DS 21.421 DYE STAINS OF SPECIAL APPLICATION 425 



21.412 Weigert 1887 8645, 6 :228 



STOCK solutions: I. abs. ale. 82, aniline 22, nietliyl violet to sat.; II. sat. aq. sol. methyl 



violet 

 REAGENTS KEQUiREu: A. stock I 9, stock II 81; B. 0.6% hydrochloric acid; C. ADS 12.2 



Gram 1880; D. aniline 60, xylene 30 

 method: [sections]^ water -^ A, 5-10 mins. — » B, wash -^ C, 5 mins. — * blot — » D, 



till differentiated — > blot —> xylene — >• balsam 

 RECOMMENDED FOR: fibrin. 

 note: Mallory 1938, 193 substitutes equal parts aniline and xylene for D above; he 



further suggests prior staining of nuclei with carmine. Both Mallory {loc. cit.) and 



Schmorl 1928, 158 recommend avoiding chrome or dichromate fixation. Unna {lest. 



1928 Schmorl, op. cit., 160) substitutes 1.5% gentian violet in 10% alum for A al)ove. 



21.42 DESIGNED TO DIFFERENTIATE TYPES OF CELLS 



SI. 421 In Pituitary 



21.421 Berblinger and Bergdorf 1935 7802, 15:381 



REAGENTS REQUIRED: A. 1% cresofuschiu; B. DS 11.21 Grenacher 1879; C. water 100, 

 phosphomolybdic acid 1, orange G 2; D. 5% phosphomolybdic acid; E. 0.2% anilin 

 blue 



method: [sections of formaldehyde-fixed material] —> A, 12 hrs. — » water, quick rinse 

 -^ C, 5 mins. -^ water, quick rinse -^ D, 2 mins. — * blot, or wipe around sections — > 

 E, 15 mins. — > water, quick rinse -^70% ale, till no more color comes away — » bal- 

 sam, via usual reagents 



RECOMMENDED FOR: pregnancy cells in hypophysis. 



result: pregnancy cells, blue with yellow granules; basophil cells, blue with purple 

 granules. 



21.421 Biggart 1935 7599, 81 :42 



REAGENTS REQUIRED: A. 0.5% eosiu Y 50, 0.3% isamine blue 50; B. 95% ale. 80, 5% 



sodium carbonate 20 

 method: [sections of F 3700.1010 fixed material] —^ water -^ A, 50°C., 30 mins. — > rinse 



— > B, till differentiated — ♦ neutral mountant, via usual reagents 

 recommended for: differentiation in cell types in the pituitary. 

 result: basophil cells, blue; chromophobe cells, light blue; acidophile cells, red. 



21.421 Cleveland and Wolfe 1932 763, 51 :410 



REAGENTS required: A. DS 11.123 Ehrlich 1886; B. 5% potassium dichromate; C. 5% 

 erythrosin; D. water 100, phosphomolybdic acid 1, orange G 2; E. 1% anilin blue 



method: [2 to 3 m sections of material fixed in F 7000.1000 Regaud 1910] —> A, 3 mins. 

 — > water, quick rinse — > B, in dark, 3 days, changed daily -^ water, rinse — > C, 20-30 

 sees. — > water, rinse -^ E, 30-60 sees. — > balsam, via usual reagents 



21.421 Colin 1923a 6630, 89:1230 



formula: sat. aq. sol. aniline 100, acid fuehsin 12, light green 6 



method: [sections of formol material] —» water —> A, 2 mins. at 40°C. —♦ water, till 

 differentiated — * balsam, via usual reagents 



21.421 Colin 1923b 6630, 89:1230 



formula: water 100, aniline to sat., acid fuehsin 16, methyl blue 2.8 

 method: as Colin 1923a 



21.421 Crook and Russel 1935 11431, 40:256 



REAGENTS REQUIRED: A. water 100, potassium dichromate 2.4, acetic acid 4.75; B. 



ADS 12.2 Lugol (1905); C. 1% acid fuehsin; D. DS 13.41 Mallory 1901, sol. C 

 method: [sections] -* water— > .4, 12-18 hrs. -^ wash —> B, 3 mins. -> 95% ale, till 

 decolorized -^ C, 15 mins. — » wash -^ D, 20 mins. -^ wash ^95% ale., till differenti- 

 ated — > balsam, via usual reagents 



21.421 Dawson and Friedgood 1938 20540b, 13:17 



REAGENTS REQUIRED: A. 3% potassium dichromate; B. water 99, acetic acid 1, azo- 

 carmine 0.2; C. 1% aniline in 95% ale; Z). 1% acetic acid in 95% ale; E. 5% phospho- 

 tungstic acid; F. water 100, acetic acid 8, anilin blue 0.5, orange G 2 



