426 METHODS AND FORMULAS DS 21.421 



method: [sections from F 3000.1000 Dawson and Friedgood 1938]— > ^1, 12 hrs. -^ 

 rinse -^ B, I hr., 55°C. -^ rinse — > C, till "carmine cells" only remain red — ^ D, rinse 

 —> rinse —> E, 2 lirs. — + rinse -^ F, 12-36 hrs. -^ rinse ^ 95% ale, balsam, via usual 

 reagents 



RECOMMENDED FORI differentiation of two classes of acidophile cells in the pituitary. 



21.421 KonefE 1938 20540b, 13:49 



REAGENTS REQUIRED: A. 0.1% aniline in 90% ale; B. 1% acetic acid in 90% ale; C. 



water 100, acetic acid 1, azocarmine 1; D. 0.06% aniline in 90% ale; E. 5% phospho- 



tungstic acid; F. water 100, phosphotungstic acid 0.05, oxalic acid 2, orange G 2, 



anilin blue 0.5; G. \% acetic acid 

 method: [3-4 ix cellulose sections of F 3700.1000 fixed material, attached to slide and 



cellulose dissolved away] -^ 70% ale -^ A, 45 mins. — * B, 1-2 mins. — ^ C, 2 hrs. 



56°C. — * wash -^ D, till nuclei red, cytoplasm pink -^ B, 1-2 mins. — * j^, 4 hrs. — > 



rinse -^ F, A hrs., till basophils blue — » wash — > E, 3-5 mins. -^ wash -^ G, rinse -^ 



wash — > neutral mountant, via usual reagents 

 RECOMMENDED FOR: differentiation of basophils (blue), acidophils (orange red) and 



chromophobes (light gray). 



21.421 Kraus 1912 2526, 54 :520 



REAGENTS REQUIRED: A. 5% potasslum dichromate; B. DS 11.113 Kultschitzky 1889; 



C. ADS 21.1 Weigert 1885 

 method: [5 M paraffin sections of formaldehyde-fixed material] -^ A, overnight, 37.5°C. 



-» thorough wash^ B, 24 hrs. -> wash-^' C, till /3-cells decolorized -> DS 12.221 



counterstain, if desired — > balsam, via usual reagents 

 RECOMMENDED FOR: differential staining of a-cells (black) in hypophysis. 



21.421 Lewis and Miller 1938 20540b, 14:111 



This is identical with DS 13.41 Kricheski 1931, save that the time in the A and B solu- 

 tions is increased to 30 minutes and 24 hours respectively. The authors make the com- 

 mon error of referring to Kricheski's formula for the B solution as "Mallory's stain." 



21.421 Lillie 1948 Lillie 1948, 99 



formula: water 90, citric /phosphate buffer, pH 8.5, safranin O 0.05, eriocyanine A 0.05 

 method: [sections] —* water —> stain, till differentiated —> rinse ^ acetone, till de- 

 hydrated -^ balsam, via xylene 

 result: acidophile cells, l)lue; basophiles, red. 



21.421 MacCallum, Futcher, Duff, and Ellsworth 1935 10919, 56:350 



reagents required: A. sat. sol. cupric acetate; B. 0.4% ripened hematoxylin; C. 3% 



potassium dichromate; D. ADS 21.1 Weigert 1885 

 method: [paraffin sections of F 3700.1000 Helly 1903 material] -^ water -^^ A, 5 mins. — » 



wash -^ B, \ min. -^ wash — » C, 1 min. -^ wash — > D, till differentiated — > wash — > 



balsam, via usual reagents 

 recommended for: differentiation of anterior lobe from pars intermedia cells in the 



pituitary. 

 result: anterior lobe cells with black granules; granules unstained in pars intermedia 



cells. 



21.421 Martins 1933 6630,113:1275 



reagents required: A. DS 11.123 Harris 1900; B. 0.1% acid fuchsin; C 1% phospho- 



molybdic acid; D. 0.5% methyl blue 

 method: [sections of F 3700.1000 Helly 1903] -^ water -^ A, 2-3 mins. -^ wash -> B, 



10 sees. — > rinse — + C, 1-2 mins. -^ drain — > D, 2-3 mins. — > balsam, via usual 



reagents 



21.421 Maurer and Lewis 1922 11189, 36:141 



reagents required: A. 1.7% acid fuchsin 70, sat. aq. sol. "acid violet" 30; B. clove oil 



75, abs. ale 25 

 method: [sections from F 3600 fixed material] -^ water — > stain, 20-30 sees. — > blot -^ 



acetone — ^ benzene -^ B, till differentiated — ^ benzene -^ balsam 

 recommended for: differentiation of cell types in pars intermedia of pituitary. 



