DS 21.421 DYE STAINS OF SPECIAL APPLICATION 427 



21.421 Maxwell 1938 2054Ub, 13:93 



REAGENTS REQUIRED:/!. 1% acid fuchsin; B. 0.02% ammonia; C. 0.1% hydrochloric acid; 



D. 0.5% phosphomolybdic acid; E. water 100, aniliii blue, orange G 2, phospho- 

 molybdic acid 1 



method: [3-5 m sections in E 21.1 Maxwell 1938 of F 3700.1010 Heidenham 1916 fixed 

 material] — > water — > A, 30 mins. — » rinse — > B, till differentiated -^ C, few moments 

 — » D, 3 mins. — > E, 1 hr. — > rinse -^95% ale, till differentiated — * abs. ale. — » bal- 

 sam, via S 41.1 Maxwell 1938 



21.421 Perry and Lochead 1939 4349, 19:101 



reagents required: A. 3% potassium dichromate; B. 0.1 aniline in 90% ale; C. 1% 

 acetic acid in 90% ale; D. 1 % azocarmine in 1 % acetic acid; E. 90% ale. 30, B (above) 

 GO; F. 5% phosphotungstic acid; G. water 100, oxalic acid 2, phosphotungstic acid 

 0.05, orange G 2, anilin blue 0.5 

 method: [4 M sections of F 3700.1010 Heidenhain 1916 fi.xed material] — > water — * A, 12 

 hrs. — » rinse — > £, 4 hrs. 56°C. followed by 14 hrs. room temperature — > wash -^ C, 

 till nuclei differentiated -^ D, 1-2 mins. -^ E, 4: hrs, — > rinse — * F, 4 hrs. — > wash -^ 

 G, 5-10 mins. -^ wash -^ C, 1-2 mins. — > wash — > abs. ale, till differentiated — > 

 balsam, via xylene 

 recommended for: pituitary of mouse. 



21.421 Pearse 1950 20540b, 25 :97 



reagents required: A. ADS 11.1 Hotchkiss 1948 (A and B sols.); B. DS 11.43 de 

 Tomasi 1936; C. DS 11.41 Lendrum and McFarlane 1940; D. DS 11.122 Mayer 1896; 



E. 2% hydrochloric acid in 70% ale; F. water 100, phosphotungstic acid 5, orange 

 G2 



preparation of f: Mix ingredients. Stand 48 hours and decant. 



method: [4-4 n sections F 3700.1000 Helly 1903 fixed material] -> 70% ale -^ A (A 

 sol.), 5 mins. -^ 70% ale, rinse -^ A {B sol.), 1 min. -^ 70% ale, rinse — * B, 10-30 

 mins. — > thorough wash —* C, J^^-3 mins. -^ rinse -^ D, }-i to 3 mins. -^ E, 10-20 

 sees. —>■ thorough w^ash —> F, 10 sees. -^ wash, till differentiated, 5-30 sees. -^ balsam, 

 via usual reagents 



21.421 Romeis 1940 test. 1948 ips. Romeis 1948, 511 



REAGENTS REQUIRED: A. DS 21.13 Weigert 1898; B. 0.1% aniline in 95% ale; C. water 

 100, acetic acid 1, azocarmine 0.1; D. 1% acetic acid in 95% ale; E. 5% phospho- 

 molybdic acid; F. water 100, acetic acid 3, anilin blue 0.2 

 method : [5 M sections of formaldehyde, or sublimate-formaldehyde, material] -^ water 

 -^ A, till 6-cells dark blue-black-^ 95% ale, till background decolorized -+ B, 15 

 mins. — > C, 1 hr. 58°C. — > leave cool, 30 mins. —>■ B, till connective tissues decolorized 

 -^ D, quick wash —* wash -^ E, 4: mins. -^ drain and blot — * F, 40 mins. — > quick 

 rinse — » 95% ale, till no more color comes aw^ay — » balsam, via usual reagents 

 result: a-ceUs pink; /3-cells dark brown violet; 7-cells light violet; 5-cells cobalt blue. 



21.421 Severinghaus 1932 763,53:1 



REAGENTS REQUIRED: A. F 1670.0000 Severiughaus 1932; B. water 65, pyroligneous acid 

 35, chromic acid 0.6; C. 3% potassium dichromate; D. DS 22.1 Altmann 1890 sol. A.; 

 E. 1% picric acid in 30% ale; F.\% phosphomolyi)dic acid; G. water 85, 95% ale. 15, 

 methyl green 0.4, "acid violet" 0.3; H.25% ale, 75% clove oil 



method: [small pieces] -^ A, 24 hrs. -^ wash -^ B, 24 hrs. -^ wash — » C, 3 days — > wash 

 — > [3 M paraffin sections] — > D, on slide heated to steaming 2-3 changes — > wash —f E, 

 till differentiated -^ wash -^ F, 1 min. — > wash -^ G, I min. -^ wash -^ blot — > 95% 

 ale, 5 sees. — > H, till differentiated — > balsam, via xylene 



21.421 Spark 1935 11284, 20:508 



REAGENTS REQUIRED: A. 0.25% anilin blue; B. DS 11.122 Mayer 1891; C. DS 12.221 



van Gieson 1890 

 method: [sections of F 7000.1000 material] -+ A, \-\\i mins. — > w^ash, 30 sees. — > B, 



10 mins.—* wash 2-3 mins. — > C, 1-1^2 mins. — > wash 1 min. — > balsam, via usual 



reagents 

 result: /3-granules, blue; a-granules, green. 

 RECOMMENDED FOR: differentiation of cell types in pituitary. 



