428 METHODS AND FORMULAS DS 21,421-DS 21.422 



21.421 Wallraff 1939 23507a, 45:631 



REAGEXTs REQUIRED: A. ADS 12.2 Salazar 1923; B. 3% ferric alum; C. 0.5% hydro- 

 chloric acid in 70% ale; D. water 100, acetic acid 1, azocarmine 0.1; E. 0.1% aniline 

 in 95% ale; F. 1% acetic acid in 95% ale; G. 0.5% toluidine blue 



method: [5 M sections of F 5000.1000 Bouin 1897 fixed material, washed free of fixative] 

 — » water —> A, 2-3 mins. -^ wash -^ B, 30 sees. -^ wash -^ C, till /3-cells differenti- 

 ated ^ wash, 70% ale. -^ D, }i-l hr. 58°C. -^ E, till connective tissues decolorized 

 — > F, rinse — > G, 1-3 mins. 95% ale, 24 hrs. -^ balsam, via usual reagents 



RECOMMENDED FOR: differentiation of /3-cells (black) in hypophysis. 



21422 In Other Glands 



21.422 Baley 1937a 1U31, 44:272 



PREPARATION OF DRY STOCK: Mix equal parts saturated solutions of magenta and orange 



G. Filter. Wash till pale yellow. Dry. 

 REAGENTS REQUIRED: A. ADS 12.2 Lugol (1905); B. DS 11.123 Ehrlich 1886; C. 0.1% 



hydrochloric acid in 70% ale; D. water 50, sat. sol. dry stock in abs. ale 50 

 method: [sections of F 3700.1000 Baley 1937 fixed material] -^ abs. ale — » A, 2 mins. 



-^ abs. ale, wash — > B, 20 mins. -^ wash -^ C, till pink — > tap water, till blue —> D, 



on slide, warmed to steaming, 1 min. — > blot — > abs. ale rinse — » A, till red, 10 sees. 



— > blot -^ abs. ale — * balsam, via xylene 

 recommended for: zymogen granules (red) in pancreas. 



21.422 Baley 1937b 11431,44:272 



PREPARATION OF DRY STAINS: T. Mix equal parts saturated solutions of magenta and 

 methylene blue. Filter. Wash ppt. till wash-water pale blue. Dry. II. As I, substitut- 

 ing "acid violet" for methylene blue. 



REAGENTS REQUIRED: A. ADS 12.2 Lugol (1905); B. water 50, sat. sol. either I or II in 

 abs. ale 50 



method: [sections of F 3700.1000 Baley 1937 fixed material] — * abs. ale — > A, 2 mins. — > 

 abs. ale, wash — > B, on slide, warmed to steaming, 1 min. —> blot — > abs. ale, rinse -^ 

 A, till red, 10 sees. -^ blot -^ abs. ale -^ balsam, via xylene 



recommended for: differentiation of ^-cells (red with I, violet with II) from a-cells 

 (blue with I, unstained with II) in islets of Langerhans. 



21.422 Baley 1937c 11431,44:272 



REAGENTS REQUIRED: A. ADS 12.2 Lugol (1905); B. 1% eosin; C. 1% acetic acid; D. 



water 100, acetic acid 0.5, anilin blue 0.375, orange G 0.625, resorcin 0.75 

 method: [sections of F 3700.1000 Baley 1937 fixed material] -^ abs. alc.,-^ A, 2mins. — > 



abs. ale, wash — > B, 10 mins. — > blot — > ( ', 2 mins. -^ D, 1 min. -^ blot — > abs. ale, 



rinse -^ A, 10 sees. -^ blot -^ abs. ale — > balsam, via xylene 

 recommended for: differentiation of zymogen granules (bright red); granular cells of 



acini (dark red) ; )3-cells (red) ; a-cells (blue) in pancreas. 



21.422 Bensley 1916 590, 19:37 



REAGENTS REQUIRED: A. 1% ammouium chlorostannate; B. DS 11.3 Bensley 1916; C. DS 



12.16 Bensley 1916 

 method: [sections of F 3700.1000 fixed thyroid] — > water —> A, 2 mins. -^ water, quick 



rinse -^ B, 1-3 hrs. -^ water, wash — ^ C, 1-5 mins. — ^ water, quick rinse — » abs. ale 



— > balsam, via xylene 

 RECOMMENDED FOR: differentiation of glandular elements of thyroid. 



21.422 Bowie 1925 763,29:57 



PREPARATION OF DRY STOCK: Mix 60 sat. sol. ethyl violet with 30 sat. sol. Biebrich 



scarlet. Filter. Wash and dry ppt. 

 REAGENTS REQUIRED: A. water 80, ale 20, dry stock 0.001; B. cloA^e oil 75, abs. ale 25 

 method: |3 /u sections of F 3700.0010 Bowie 1925 fixed material] — > water -^ A, 24 hrs. 



-^ acetone, rinse — > B, till differentiated — » acetone — * toluene — » balsam 

 RECOMMENDED FOR: differentiation of cell types in pancreas. 

 result: a-cells, blue; 7-cells, red; ^-cells purple. 



