DS 21.422 DYE STAINS OF SPECIAL APPLICATION 429 



21.422 Fujiware 1939 1887a, 27:1030 



STOCK solutions: I. 1% ponceau 2R in 1% acetic acid; II. 1% acid fuchsin in 1% 



acetic acid 

 REAGENTS REQUIRED: A. stock I 00, stock II 30; B. sat. aq. sol. picric acid 

 method: [sections of F 3700.1010 lleidenhain 1916 material] — > A, 5 mins. — > rinse —>■ B, 



5 mins. — » balsam, via usual reagents 

 recommended for: androgenic cells in adrenal cortex. 



21.422 Gomori 1939 763,74:439 



REAGENTS REQUIRED: A. watcr 100, azocamiinc 0.1, acetic acid 2; B. 1% aniline in 90% 

 ale; C. 5% ferric alum; D. water 100, orange G 0.2, anilin blue 0.6 



method: [sections of F 5000.1010 Benin 1897 fixed material] -^ water — > A, 45-60 mins., 

 55°C. -^ rinse — ^ blot — » B, till acinous cells decolorized — > rinse -^ C, 5 mins. — > rinse 

 — » D, till collagen deep blue -^ abs. ale, till differentiated — » balsam, via xylene 



RECOMMENDED FOR: differentiation cell types in islets of Langerhans. 



result: a-cells, orange; jS-cells, red; 6-cells, blue. 



21.422 Gomori 1941 608b, 17:395 



REAGENTS REQUIRED: A. F 5000.1010 Bouiu 1897; B. water 100, sulfuric acid 0.3, potas- 

 sium permanganate 0.3; C. 3% sodium bisulfite; D. DS 11.124 Gomori 1941; E. 1% 

 hydrochloric acid in 70% ale; F. 0.5% phloxine; G. 5% phosphotungstic acid 

 method: [sections of F 5000.1010 Bouin 1897 fixed material]-^ water -^ A, 1 day — > 

 thorough wash -^ B, 1 min. -^ C, till bleached -^ wash — » Z), 10-15 mins., till cells 

 deep blue -^ E, 1 min. —>■ tap water, till blue -^ F, b mins. -* rinse — > G, 1 min. 95% 

 ale, till differentiated -^ balsam, via usual reagents 

 recommended for: differentiation of a, /3 and 6 cells of pancreas. 



21.422 Lane test. 1937 Duthie Gatenby and Painter 1937, 420 



PREPARATION OF DRY STOCK: Mix 50 1.5% Crystal violet and 50 1.25% orange G. Filter, 

 wash, and dry ppt. 



PREPARATION OF STOCK SOLUTION: abs. alc. 100, dry powder from above to sat. 



reagents required: A. water 90, stock solution from above 10; B. clove oil 75, abs. 

 alc. 25 



method: [3 M sections of material fixed in either F 3700.0000 Lane (1910) (for a-cells) or 

 F 3700.0000 Lane (1937) (for /3-cells)] -^ water -^ A, 24 hrs. -^ blot -^ acetone, till de- 

 hydrated — ^ toluene -^ B, till differentiated — * balsam, via toluene 



recommended for: differentiation of a and j3 cells in islets of Langerhans. 



21.422 Launoy 1904a test. 1907 Bohm and Oppel Bohm and Oppel 1907, 355 



REAGENTS REQUIRED: A. DS 11.122 Launoy 1904; B. 0.1% hydrochloric acid in 70% ale; 



C. DS 11.43 Ziehl 1882; D. 0.1% light green in 70% alc. 

 method: [sections of osmic, chrome, or platinic fixed material] — > A, 15 mins. -^ wash — > 



B, till nuclei alone colored -^ wash -^ C, on slide, warmed to steaming, 5-10 mins. -^ 



wash, till no more color comes away — > D, few moments -^ B, till differentiated — > 



balsam, via usual reagents 

 RECOMMENDED FOR: cellular differentiation in pancreas. 



21.422 Launoy 1904b lest. 1907 Bohm and Oppel Bohm and Oppel 1907, 357 



REAGENTS REQUIRED: A. DS 11.122 Lauiioy 1904; B. 0.1% hydrochloric acid in 70% 



ale; C. DS 11.42 Zwaademaker 1887; D. sat. aq. sol. orange G 

 method: [sections] -^ A, 15-20 mins. -^ B, M to 1 hr. -^ C, 48 hrs. -^ wash till no more 



color comes away — > D, few moments—* 95% ale, wash — > B, till differentiated-* 



balsam, via usual reagents 

 RECOMMENDED FOR: cellular differentiation in pancreas. 



21.422 Lillie 1948 see DS 21.422 Wiesel 1903 (note) 



21.422 Miiller 1950 Mikroskopie, 6:245 



REAGENTS REQUIRED: A. watcr 100, potassium permanganate 0.125, sulfuric acid 0.25, 

 B.2% oxalic acid; C. water 100, zinc acetate 0.5, Bismarck brown 0.5; D. 0.6 acetic 

 acid; E. 0.5% light green 



