430 METHODS AND FORMULAS DS 21.422-DS 21.423 



method: [3-5 n sections of F 5000.1010 Bouin 1896 fixed material]^ waters A, 2 

 mins. -^ wash -^ B, till white — > wash -> C, 2 mins. — * rinse —> D, till differentiated 

 — > wash —> E, 2 mins. -^ wash — > balsam, via usual reagents 



RECOMMENDED FOR: differentiation of a (green) and (brown) cells in islets of Langer- 

 hans. 



21.422 Wiesel 1902 764, 19 :481 



REAGENTS REQUIRED: A. 1% toluidine blue; B. 1% safranin 

 method: [sections of F 7000.1000 Wiesel 1902 material] —+ water —» A, 20 mins. — > 



wash, 5 mins. running water — > B, 20 mins. -^95% ale, till again blue — > balsam, via 



carbol-xylene 

 RECOMMENDED FOR: demonstration of chromaffin cells in adrenal. 

 result: nuclei, red; chromaffin cells, green; other cells, blue. 

 note: Lillie 1948 (p. 104) cites Schmorl (no reference) in substituting anilin blue for 



toluidine blue in A above and in specifing "chromate fixed material." 



21.422 WiUiamson and Pearse 1923 11025, 57:193 



reagents required: A. ADS 12.1 Lugol (1905); B. 0.25% potassium permanganate; 



C. 5% oxalic acid; D. DS 11.124 Mallory 1900 

 method: [sections of F 3670.0000 Williamson and Pearse 1923 fixed material]—* water 



-^ A, 30 mins. —♦95% ale, wash — ♦ B, 10 mins. —♦ rinse —> C, till decolorized—* 



wash — > D, 24 hrs. —* wash -^ balsam, via usual reagents 

 recommended for: differentiation of cell types in thyroid. 



21423 In Other Structures 



21.423 Coutelin 1931 899a, 9:188 

 reagents required: A. 0.5% acid fuchsin; B. 1% phosphomolybdic acid 



method: [20 n sections from formaldehyde-fixed material] — > water — > A, 5-10 mins. — > 



water, wash — > B, 10-20 sees. -^ balsam, via usual reagents 

 RECOMMENDED FOR: differentiation of flame cells. 

 result: nuclei, and cilia, of flame cells, red. 



21.423 Downey 1913 see DS 21.423 Wright 1910 (note) 



21.423 Endicott 1945 20540b, 20 :5 



REAGENTS REQUIRED: A. water 60, DS 11.122 Delafield (1885) 30; B. 1% hydrochloric 

 acid in 95% ale; C. 1% sodium phosphate dibasic; D. water 96, citric acid 0.212, 

 sodium phosphate, dibasic (cryst.) 0.241, DS 13.13 Endicott 1945 (working sol.) 4 

 method: [nitrocellulose sections of F 7000.1000 Orth 1896 fixed, and formic acid decalci- 

 fied, material] — > water —* A, 5 mins. -^ B, rinse 95% ale, quick wash — » C, till blue 

 — > wash -^ D, 1 hr. — >• E, till differentiated — ♦ balsam, via usual reagents 

 RECOMMENDED FOR: differentiation of cell types in bone marrow. 



21.423 Fuller 1943 11284, 28:1475 



reagents required: A. water 50, DS 11.123 Ehrlich 1886 50; B. water 99, acetic acid 1, 



ponceau 2 R 0.5, acid fuchsin 0.5; C. water 100, phosphomolybdic acid 1, orange G 2 

 method: [ale fixed smears] — > water —♦ A, 2 mins. — » wash, 4 mins. -^ B, 2 mins. —> 



rinse —* C, 2 mins. -^ balsam, via usual reagents 

 RECOMMENDED FOR: vaginal smears. 



21.423 Gomoril946 Tech. Bull., 7 -AB 



REAGENTS REQUIRED: A. 0.05% azocamiinc in 1% acetic acid; B. 1% aniUne in 95% 



ale; C. 3% phosphotungstic acid; D. water 100, oxalic acid 2, methyl blue 0.5, 



tartrazine 2 

 method: [sections of material after other than dichromate fixation] -^ water -^ A, 1-lJ^^ 



hrs., 60°C. -^ rinse -^ blot -> 95% ale, rinse -> B, till chromaffin cells deep pink -> 



rinse —> C, 20 mins. -^ quick wash -^ D, 15-40 mins., till collagen deep blue -^ rinse 



-^ balsam, via usual reagents 

 recommended for: demonstration of chromaffin granules (purple to ruby-red). 



