DS 21.423 DYE STAINS OF SPECIAL APPLICATION 431 



21.423 Hamazaki 1935 22575, 295:703 



REAGENTS REQUIRED: A. Water 95, abs. ale. 5, phenol 2.8, magenta 0.5; B. 1% hydro- 

 chloric acid; C. ADS 12.2 Lugol (1905); D. 1% sodium thiosulfate; E. 3% hydro- 

 chloric acid 

 method: [6 M sections of F 3700.1010 Hamazaki 1935 fixed material]^ water— > A, 1 

 hr. -^ wash -^ B, 10 mins. — > C, 30 mins. —> rinse — > D, till iodine-free -^ wash — » E, 

 30 mins. -^ wash -^ balsam, via usual reagents 

 recommexded for: demonstration of wandering cells (red) in smooth muscle. 



21.423 Hoecke and Sebruyns 1952 20540b, 27 :263 



REAGENTS REQUIRED: A. sat. sol. Bismarck lirown in 70% ale; B. sat. sol. anilin blue in 

 2.5% acetic acid; C. 0.1% methyl violet; D. water 30, 95% ale. 70, aluminum chloride 

 0.1, hematein 0.2; E. 95% ale. 100, saffron 2; F. 0.1% acetic acid in abs. ale. 



PREPARATION OF E: Digest 6 hours at GO°C. Filter. 



method: [sections] —> 70% ale. — > A, 3 mins. — > 70% ale., rinse —> water, wash—* B, 

 2 hrs. -^ C, dropped on slide, 2 mins. — > 70% ale, wash — > D, 30 mins. -^70% ale, 

 rinse —* E, till differentiated -^ F, 30 sees. -^ balsam, via usual reagents 



recommended for: differential staining of gastric glandular cells. 



21.423 Kingsley 1937 8545, 57:87 



reagents required: A. DS 13.13 Kingsley 1935 (sol. A); B. 0.008% acetic acid; C. 

 acetone 100, eosin Y 0.001, acetic acid 0.04 



method: [sections of F 3500.1000 Kingsley 1937 fixed material] — > water, via butyl ale. 

 — > A, 8-10 mins. -^ rinse -^ B, wash -^ rinse -^ blot -* C, rinse — ♦ "neutral mount- 

 ant," via butyl ale. and xylene 



recommended for: demonstration of megakaryocytes in sections of bone marrow. 



21.423 Levine 1928 11284, 14:172 



reagents required: A. 1% thionin; B. sat. sol. orange G in clove oU 



method: [5 M sections] — ^ water -^ A, 5 mins. -^ water, till no more color comes away — > 



blot -^ B, few moments -^ clove oil, till differentiated -^ balsam, via xylene 

 RECOMMENDED FOR: mast cells in connective tissue of parathyroid. 

 result: mast cells blue; other structures orange. 



21.423 Novak 1910 766,36:217 



REAGENTS REQUIRED: A. DS 12.212 Unna 40, 1% eosin in 80% ale. 40, 1% hydro- 



quinone 12, orcein 0.4; B. 1% safranin; C. 0.5% potassium dichromate 

 method: [sections] -^ water — » A, 5-10 mins. -^ B, 10 mins. — > wash -^ C, 30 mins. —>■ 



■wash -^ balsam, via usual reagents 

 RECOMMENDED FOR: demonstration of corpuscles of Herbst. 



21.423 Ralston and Wells 1939 591b, 3:72 



REAGENTS REQUIRED; A. Water 100, azocarmine 1, acetic acid 1; B. 0.15% aniline in 

 95% ale.; C. 0.07% acetic acid in 95% ale; D. 0.5% toluidine blue 



method; [4 M sections of bone decalcified in AF 21.1 Ralston and Wells 1939] -^ water — > 

 A, 20 mins., 56°C. -^ B, till color clouds cease -^ C, till rose red -^ wash — > D, 10-15 

 sees. — * abs. ale, least possible time for dehydration — >• balsam, via xylene 



RECOMMENDED FOR: differential staining of bone marrow. 



21.423 Shoor 1941 19938,94:545 



formula: water 50, 95% ale 50, Biebrich scarlet 0.5, orange G 0.25, fast green FCF 



0.075, phosphotungstic acid 0.5, phosphomolybdie acid 0.5, acetic acid 1 

 method: [alcohol-ether-fixed smears] -+ stain, 1 min. — > 70% ale, rinse -> dammar, via 



usual reagents 

 recommended for: vaginal smears. 



21.423 Papanicolaou 1941 11284,26:1200 



reagents required: A. DS 11.123 Ehrlieh 1886; B. water 100, phosphotungstic acid 



0.112, phosphomolybdie acid 0.225, anilin blue W.S. 0.06, orange G 0.125, acid 



fuchsin 0.1, eosin Y 0.21 

 method; [alcohol-fixed smears] — » water —> A, 2 mins. — > rinse —»• "blue" in tap water 



—> rinse —^ B, 2-5 mins. -^ rinse — + balsam, via dioxane 

 recommended for: vaginal smears. 



