DS22.il DYE STAINS OF SPECIAL APPLICATION 435 



22.11 Barrett 1932 20540b, 7:63 



formula: water 25, 95% ale. 25, acctie acid 50, hematoxylin O.OG, ferric alum 0.5 

 method: [smear preparations]-^ stain, under coverslip, till chromosomes clear —» seal 



cover 

 RECOMMENDED FOR: poUen mother cells. 



22.11 Bradley 1948 20540b, 23:29 



REAGENTS REQUIRED: A. F 0000.0010 Bradley 1948; B. 4% ferric alum; C. 50% hydro- 

 chloric acid; D. DS 11.23 BeUing 1921 

 method: [plant ovaries] —> A, 2 days — > B, 75°C., 3 mins. -^^ water, 75°C. 2 mins. — > 

 fresh water, 75°C., 2 mins. — > cold water, 2-3 mins. —* C, 10 mins. -^ thorough wash 

 —> [scrape ovules from placenta to slide] -^ [apj)ly cover and top to separate cells] 

 — > heat to steaming -^ jar filled witii ale. vapor, 24 hr.s. —> abs. ale, 1 drop run under 

 cover -^ spread M 23.1 mountant round edge of cover, leave in jar with slight ale. 

 vapor content 24 hrs. — > dry 



22.11 Bizzozero-Vassale test. 1894 Kahlden and Laurent 



Kahlden and Laurent 1894, 71 

 REAGENTS REQUIRED: A. DS 23.211 Ehrlich 1882; B. ADS 12.2 Gram 1884; C. 1% 



chromic acid 

 method: [sections] — ^ A, 10 mins. — * abs. ale, rinse —> B,2 mins. — > abs. ale, 30 sees. — > 

 C, 30 sees. -^ abs. ale, 30 sees. -^ C, 30 sees. —* abs. ale, 30 sees. — » clove oil, till no 

 more color comes away — » balsam, via usual reagents 



22.11 Conn 1943 20540b, 18:189 



REAGENTS REQUIRED: A. \% chlorazol blaclc E 



method: [sections of root tips] — + water —* A, 2 hrs. —> balsam, via usual reagents 



22.11 Cooper 1931 591, 18:337 



REAGENTS REQUIRED: A. 1% methyl green; B. 1% acid fuchsin; C. 1% erythrosin 

 method: [sections] —* water —> a, 1 hr. -^ water, rinse— *B, 1 min. -^ water, quick 



rinse -^ C, few sees. — > abs. ale, minimum possible time —>■ balsam, via xylene 

 result: chromatin, green; linin, red. 



22.11 Dalton test. 1948 Lillie Lillie 1948, 87 



REAGENTS REQUIRED: A. water 55, acetic acid 1, orcein 45; B. 0.15% fast green FCF in 



abs. ale 

 PREPARATION OF a: Dissolvc dye in hot acid. Cool. Dilute to 100 with water. 

 method: [pieces for chromosomes]—* A, 48 hrs. — > fragments crushed under coverslip 

 on slide coated with V 21.1 Mayer 1884—* ale vapor, 48 hrs. -^ 95% ale, few mo- 

 ments -^ B, few sees. — > balsam, via usual reagents 



22.11 Darrow 1944 20540b, 19 :65 



REAGENTS REQUIRED: A. 1% safranin O; B.sat. sol. {circ. 1%) anilinblue W Sin 95% ale 

 method: [sections from F 6000.1010 fixed material] — > water — > A, 15 mins. — » rinse —* 



B, 2 mins. — ^ balsam, via usual reagents 

 recommended for: chromosomes in root tips. 



22.11 Geither 1940 test. 1948 Romeis Romeis 1948, 223 



reagents required: A. 2% osmic acid; B. F 1600.0010 Benda 1901; C 3% hydrogen 



peroxide; D. 1% safranin; E. 0.2% light green 

 method: [fresh smears] — » A, 10-20 sees. — > B, 10 mins. —> thorough wash —y C, 10 mins. 



-^ thorough wash — * D, 10 mins. — > rinse —> E, i'4-l min. — * 95% ale — > balsam, via 



usual reagents 

 result: chromatin, nucleoli, red; centrosome, spindle fibers, green. 



22.11 Hancock 1942 20540b, 17 :79 



reagents required: A. 1% chromic acid; B. 0.1% crystal violet; C. 1% iodine and 1% 



potassium iodide in 80% ale 

 method: [10 IX sections of F 6000.1010 Belling 1928 fixed material]-^ water -^ A, 20 



mins. —> 95% ale, rinse —> abs. ale, least possible time —+ clove oil, till clear —> 



xylene, 2 hrs. — > balsam 

 recommended for: minute plant chromosomes. 



