436 METHODS AND FORMULAS DS22.il 



22.11 Hermann 1893 test. 1905 Bohm and Davidoff Bohm and Davidoff 1905, 76 



KEACiKNTs REQUIRED: A. 1% safraiiiii; B. sat. sol. gentian violet in sat. sol. aniline; C. 



ADS 12.2 Lugol (1905) 

 method: [sections from F 1200.0010 Hermann 1889 fixed material] -^ water -^ A, over- 

 night — * B, 3-5 mins. -h. rinse -^ C, till uniform black -^95% ale. till violet -^ clove 

 oil —> examine —> [repeat 95% ale. if insufficiently differentiated] ^^ balsam, via 

 xylene 

 result: chromosomes, bluish violet; nucleoli, aster, spindle fibers, red. 



22.11 Hruby 1933 19938, 77:352 



REAGENTS REQUIRED: A. sat. sol. {circ. 1%) magenta; B. sat. sol. {circ. 1.2%) picric acid 



50, sat. sol. (circ. 2%) indigo-carmine 50 

 method: [sections] — » water — > A, 5-20 mins. — > water, thorough wash —> B, 5-10 mins. 



— ^ 70% ale, rinse —^ abs. ale, till green—* balsam, via usual reagents 

 result: chromosomes, red; nucleoli, blue; spindle fibers, dark blue. 



22.11 Johansen 1932 20540b, 7:17 



reagents required: A. 1% methyl violet 2B; B. sat. sol. {circ. 1.2%) picric acid in 95% 

 ale; C. 0.1% ammonia in 95% ale; D. abs. ale 50, clove oil 50, erythrosin to sat. 

 method: [sections] -^ water -^ A, 15 to 30 mins. — > water, quick rinse -^ B, till differ- 

 entiated, about 15 sees. -^ C, 15 sees. — > 95% ale, thorough rinse -^ D, 5-10 sees. — > 

 clove oil, 15 sees. — > balsam, via xylene 

 result: chromatin purple, plastin red. 



22.11 Kedrovsky 1931 23632,47:433 



reagents required: A. water 100, orange G 0.6, rose bengal 0.3, phosphomolybdic acid 

 0.01; B. 1% toluidine blue 



PREPARATION OF A: Dissolve the rose bengal in 50 boiling water. Add 11% phospho- 

 molybdic acid slowly and with constant agitation. Cool and add orange G dissolved 

 in 50 water. 



method: [sections or smears]—* A, 20-30 mins. -^ water, rinses B, 5 mins. -^ 70% 

 ale, 1 min. -^95% ale, till differentiated -^ balsam, via bergamot oil 



result: chromatin, dark blue; nucleoli, bright red; spindle fibers, pink against blue. 



note: a detailed description of the use of this stain is given under DS 22.10 above. 



22.11 Kurnick and Ris 1948 20540b, 23:17 



formula: water 55, 95% ale 9, acetic acid 36, orcein 0.8, fast green 0.1, sodium chloride 



0.4 

 method: [fresh smears, or mounted sections, taken to water] — >• stain, few moments -^ 



balsam, via usual reagents 

 result: chromatin, red brown; cytoplasm, nucleoli, green. 



22.11 Kurnick and Ris 1948 20540b, 23:17 



formula: water 55, acetic acid 36, 95% ale 9, orcein 0.8, fast green FCF 0.1 sodium 



chloride 0.4 

 method: [smears and squashes]—* A, under cover, few mins. ^ remove cover—* bal- 

 sam, via usual reagents -^ replace cover 

 result: chromatin, brownish red; nucleoli, green. 



22.11 La Cour 1941 20540b, 16:169 



REAGENTS REQUIRED: A. watsr 55, acetic acid 45, orcein 1; B. 10% acetic acid 

 method: [fresh smear]—* A, under coverslip, 2-3 mins.—* invert slide in B till cover 

 falls off -^ balsam, via usual reagents 



22.11 Maneval 1934 19938,80:292 



REAGENTS REQUIRED: A. DS 11.43 Maueval 1928; B. 0.01% hydrochloric acid in 95% 



ale C. clove oil 100, light green 0.075, orange G 0.025 

 method: [sections] -^ water — * A, 3-5 mins. -+ wash — * B, till differentiated -^ abs. ale, 



till dehydrated — * C, till stained -^ balsam, via xylene 

 RECOMMENDED FOR: mitosis in plant cells. 



