DS 22,11 DYE STAINS OF SPECIAL APPLICATION 437 



22.11 McCUntock 1929 20540b, 4 :53 



REAGENTS REQUIRED: A. 25% acetic acid in abs. ale; B. DS 11.23 Belling 1921; C. 10% 

 acetic acid; D. 50% acetic acid in abs. ale; E. 10% acetic acid in abs. ale; F. abs. ale. 

 50, xylene 50 

 method: [fresh anthers]^ A, till required-* squeeze contents of anther onto slide, 

 cover with B, crush — > warm to steaming — > cool — + repeat warming 3 or 4 times —* 

 C, in petri -^ E, 2 mins. — > abs. ale, 2 mins. — > F, 2 mins. — > xylene, 2 mins. — > [re- 

 combine slide and cover] -^ balsam 

 recommended for: plant chromosomes. 



22.11 Meyer 1886 see DS 11.28 Meyer 1885 



22.11 Mitter and Bartha 1948 20540b, 23 :27 



reagents required: .1. water 55, acetic acid 45, brilliant cresyl blue 0.75; B. 45% 



acetic acid 

 method: [whole salivary glands of Drosophila] — > A, in vial, 30-45 mins. -^ transfer 



glands to slide —>■ drain -^ blot — > cover and tap cover to break nuclei -^ B, under 



cover, if differentiation necessarj' -^ seal cover 

 recommended for: salivary gland chromosomes of Drosophila. 



22.11 Nissl 1894 see DS 11.113 Nissl 1894 



22.11 Rawitz see DS 11.42 Rawitz 1895 



22.11 van Rosen 1947 14900. 160:121 



reagents required: A. 30% acetic acid in 95% ale, B. 30% hydrochloric acid in 95% 



ale; C. water 50, acetic acid 50, nigrosin, ale soluble 4 

 method: [fresh tissue]-^ A, 24 hrs. (cooled for roots) -^ A, 1-10 mins. 4°C. -^ wash, 



15-30 mins. — + B, on slide, 1-2 mins. -^ blot 

 recommended for: plant chromosomes. 

 note: the dye used was probably indulin, ale sol. 



22.11 Sax 1931 20540b, 6:117 



reagents required: ^4. 1% crj-stal violet; B. water 20, 95% ale 80, iodine 1, potas- 

 sium iodide 1 

 method: [smears fixed 1-2 hrs. in F 6000.1010 Navashin 1912]-* 15% ale, thorough 

 wash — » A, 1-5 mins. — » rinse -* B, 30 sees. — » abs. ale, till differentiated —* balsam, 

 via usual reagents 

 recommended for: pollen mother cells. 



22.11 Schmorl 1928 SchmorJ 1928, 140 



reagents required: A. DS 11.122 Bohmer 1868; B. 1% safranin; C. water 100, picric 



acid 0.1, tannin 25 

 method: [celloidin sections of ale fixed material]—* water —> A, 5 mins. — > tap water, 



till blue -* B, 20 mins. — > wash -^ C, tUl differentiated, 2-5 mins. — * thorough wash 



-^ balsam, via usual reagents 

 result: smiren Kerne, red; geicohnlichen Kerne, blue. 



22.11 Semmens and Bhaduri 1939 20540b, 14:1 



reagents required: A. 5% sodium carbonate; B. water 98, 95% ale 12, aniline 0.1, 



light green 0.5; C. sat. sol. sodium carbonate in 70% ale 

 method: [sections, chromatin stained by DS 11.43 de Tomasi 1936] ^^.1,1 hr. — >• wash, 



30 mins. — > B, 10 mins. — > C, rinse —> 95% ale, 10 mins. -^ repeat T^ ale cycle 



till cytoplasm free of green —> balsam, via usual reagents 

 recommended for: differential staining of nucleoli (green) and chromosomes (purple). 



22.11 Smith 1934 20540b, 9:95 



reagents required: A. ADS 12.2 Lugol (1905); B. 1% crystal violet; C. sat. sol. {circ. 



1.2%) picric acid in abs. ale 

 method: [sections or smears] — > 70% ale, — > .4, 10-20 mins. — > water, rinses B, 15 



mins. — » 95% ale, rinse — > C, few sees. —> abs. ale, till yellow removed -^ clove oil, 



till differentiated -^ xjdene, till clove oil removed — » balsam 



