438 METHODS AND FORMULAS DS 22.11-DS 22.2 



22.11 Togby 1942 20540b, 17:171 



REAGExXTs required: .4. 95% ale. 50, hydrogen peroxide (3%) 50; B. 1% chromic acid; 



C. 1% crystal violet; D.\% iodine and 1% potassium iodide in 50% ale; E. sat. sol. 



picric acid in 95% ale; F. 0.1% ammonia in 95% ale. 

 method: [sections of F 1670.0010 La Cour 1931a fixed material] -» water -^ A, 30 mins. 



-^ rinse -> B, 30 mins. -» rinse -> C, 1 hr. -^ rinse -^ D, 30 sees. — > 70% ale, rinse -> 



.E^, rinse -^ F, rinse -^ abs. ale, till dehydrated -^ clove oil, till differentiated -» 



xylene, thorough wash — > balsam 

 RECOMMENDED FOR: chromosomes of Crepis. 



22.11 Unna test. 1928 Schmorl Schmorl 1928, 139 



REAGENTS REQUIRED: A. DS 11.44 Unna 1892; B. 25% tannin 



method: [sections of F 7000.0000 Miiller 1859 or F 1600.0010 Flemming 1882 fixed 

 material] — > water -^ .l, 2 mins. — > wash — * B, 10-15 mins. -^ wash -^ balsam, via 

 usual reagents 



22.11 Wing 1930 23639b, 10:699 



REAGENTS REQUIRED: A. ADS 12.2 Wing 1930; B. 1% gentian violet 

 method: [thin sections] —> /4, 5-30 mins. -^ momentary rinse ^ B, 5-15 mins.—* 

 quick rinse — > A, 20-30 sees, differentiation — * abs. ale, least possible time for de- 

 hydration -^ clove oil, till differentiated —>■ balsam, via xylene 



22.2 Mitochondria and Golgi tinued by many firms. If it is handled in 



Apparatus the laboratory, it should be treated with 



The techniques designed for the demon- t^e respect which is accorded to any high- 



stration of mitochondria in sections so explosive material. KuU also substituted 



closely resemble those intended for the ^J'^ ^^^^^^^^ ^^ P^^^^' ^^^^.^°' ^^^ ^''^- 

 demonstration of bacteria under the same I'J^ specified by Altmann m 1890. On 

 conditions (see DS 23.22 below), that it ^^^ somewhat slender basis that Champy 



is not surprising that for many years there !™ I u^^T ""' '''^''^^\ P'^'P"'^' 

 was confusion in the literature as to the technique has been commonly known 

 whether or not mitochondria existed. The f *^^ Champy-Kull technique, and this 

 now universal acceptance of the existence *^"^, ^.^ ^°^ generally adopted in Enghsh- 

 of mitochondria has, however, unfortu- ^Peak^g countries. In France and Ger- 

 natelv permitted students to become care- 'Z""^^ however, most of what are called 

 less, and it must never be forgotten that Champy-Kull techniques in tins country 

 the bacteria which frequently grow in the f\ ".^P^^^^? . ^^ *^J ^o-M " Parat 

 egg albumen, with which sections are *''^^^"'f ' „^^ ^ "^ .^^^^^^ f -^l^' 

 attached to a slide, will stain in the same f^hromated before being stamed with a 

 colors and be of much the same size and hematoxylin stain The name Pami, how- 

 shape as mitochondria. The original tech- ^^^'' ^^J ^f^f ^^ .*°. ^^ ^PP\^^ *° .^^T'^ 

 nique, for example, of Altmann 1890, any method o staining mitochondria after 

 could be employed very readily for stain- ^eatnient with dichromate, just as the 

 ing acid-fast bacteria in almost any sec- term C/iampy-At/M has come to be trans- 

 tion of tissue terred to any technique m which acid 

 The next development in the staining of ^^^^"' 1^ ^"^ ^' *^! V^n^^rj stain. The 

 mitochondria, after Altmann, was the ^ ^^^^^,^f, ^'^f ^^^^^^^ together in 

 demonstration by Kull in 1914 that the ^^^8 by Volkonsky, who combined the 

 dye, aurantia, could satisfactorily be Pre-dichromating of Parat with the acid- 

 substituted for picric acid, to which it is fuchsin-aurantia techmque of Kull, and 

 chemically closely related, in the differ- followed these wath a double-staining 

 entiation of Altmann's stain. It may be technique using hematoxyUn, orange G, 

 remarked at this point, parenthetically, and aniUne blue by a method closely re- 

 that aurantia is even more explosive than sembling those of the Masson technicjues 

 picric acid, and is so dangerous to manu- described in sections DS 12.31 and DS 

 facture that its supply has been discon- 12.32 above. 



