442 METHODS AND FORMULAS DS 22.21 



PREPARATION OF B: Add the acid drop by drop to the dye solution until no further in- 

 crease in intensity of color takes place. 



method: [sections of F 7000.1000 Regaud 1910 fixed material] -^ A, on slide warmed to 

 steaming, 6 mins. -^ drain -^ water, quick rinse -^ B, on slide, 5 sees. -^ drain — > 

 abs. ale, till differentiated, few sees. —* carbol-xylene — > xylene -^ balsam 



22.21 Baker 1933 see DS 22.21 KuU 1914 



22.21 Baker 1944 17510, 85:1 



REAGENTS REQUIRED: A. water 90, 40% formaldehyde 10, cadmium chloride 1; B. 25% 



gelatin; C. 2.5% gelatin; D. sat. sol. Sudan black in 70% ale; E. DS 11.21 Mayer 1892 

 method: [fresh tissues]-^ A, 3 days -^ wash -^ B, 24 hrs., 37°C. ^ [cast as block] -^^ 



A, 24 hrs. -^ wash —> [15 fi frozen sections, fixed on slide with C] -^ A, till required 



wash — > 70%, ale. — > D, 5-10 mins. -^50% ale, rinse — » E, till nuclei stained —>■ wash 



-^ M 11 mountant (Chapter 26) 

 RECOMMENDED FOR: Golgi bodies. 



22.21 Baker and Thomas 1933 Baker 1933 



REAGENTS REQUIRED: A. DS 22.21 Altmann 1890, sol. A; B. water 65, ale. 35, picric acid 

 2; C. water 65, ale. 35, picric acid 2 



method: [5 M sections of F 1700.0000 Baker and Thomas 1933 fixed material] -^ water 

 -^ A, flooded on slide and heated till steaming, 1 min. — >• cool, 5 mins. -^ B, 30 sees. — > 

 C, till differentiation complete -^ wash — > balsam, via usual reagents 



22.21 Benda 1903 7936a, 12 :752 



REAGENTS REQUIRED: A. 4% ferric alum; B. water 100, sat. sol. alizarin in abs. ale. 1; 

 C. water 55, sat. sol. aniline 25, hydrochloric acid 0.025, 95% ale. 7.5, sat. sol. crystal 

 violet in 70% ale. 12.5; D. 30% acetic acid 

 method: [sections from material fixed in F 1670.0018 Benda 1903, F 1670.0080 Champy 

 1913, or F 7000.1000 Regaud 1910] -^ water -* A, 2-5 hrs. -* water, quick rinse ^ B, 

 poured on slide, heated to steaming, 1 min. -^ C, poured on slide, heated to steaming, 

 1 min. —> water, quick rinse — > D, till nuclei well defined -^ running water, 10 mins. 

 — * blot -^ abs. ale, quick rinse -^ carbol-xylene — > balsam, via xylene 

 result: cell inclusions purple on yellow. 



22.21 Bensley 1911 see DS 22.21 Cowdry 1918 (note) 



22.21 Benoit 1922 see DS 22.21 Cowdry 1918 (note) 



22.21 Cain 1948 17510,89:229 



REAGENTS REQUIRED: A. 0.5% iodine in 70% ale; B. 5% sodium thiosulfate; C. DS 

 22.21 Altmann 1890 (sol. A); D. 0.1% sodium carbonate; E. 1% hydrochloric acid; 

 F. 1 % methyl blue 

 method: [3 M sections of F3700.1000 Helly 1903 fixed material] — > water -^ A. 5 mins. 

 — » rinse — > B, 5 mins. —>■ wash —> C, on slide, heated to steaming, 1 min. -^ E, few 

 sees, [repeat D -^ E cycle if insufficiently differentiated] -^ wash — > F, till counter- 

 stained — > wash -^ E, brief dip — > balsam, via usual reagents 



22.21 Cansey 1925 21400a, 44:156 



REAGENTS REQUIRED: A. 5% fcrric alum; B. DS 11.111 Regaud 1910 (sol. B) 

 method: [osmic-fixed protozoans] — > A, 30 mins. — > rinse — > B, 45 mins. -^ A, till differ- 

 entiated — > balsam, via usual reagents 

 RECOMMENDED FOR: mitochoudria in protozoans. 



22.21 Champy-Kull see DS 22.21 Kull 1914 



22.21 Cowdry 1918 6816, 8 



REAGENTS REQUIRED: A. DS 22.21 Altmann 1890, A sol.; B. \% methjd green 

 method: [sections of F 7000.1000 Regaud 1910 material] -+ water —> .4, poured on 

 slide, warmed to steaming, G mins. -^ wipe round sections -^ water, quick rinse — ^ B, 

 on slide, 5 sees. -^ drain — ' abs. ale, till differentiated -^ balsam, via toluene 

 result: mitochondria, purple-red on green. 



