DS 22.21 DYE STAINS OF SPECIAL APPLICATION 443 



note: The method of Benoit 1922 (6(530, 86 :1 101) differs only in specifying prior fixation 

 in his F 1379.0000 fixcative; that of Bensley 1911 (590, 12:297) in requiring his F 

 1700.0010. Gough and Fulton 1929 (11431, 32:765) treat sections of formaldehyde- 

 fixed material with their ADS 12.2 mordant before this technique. 



22.21 Champy 1911 see DS 22.21 KuU 1913 



22.21 Drew 1920 11360, 40:295 



REAGENTS required: A. Drew 1920 ADS 12.1; B. 3% ferric alum; C. 0.5% hematoxylin; 



D. 2% pyridin 

 method: [sections by freezing technique of Drew 1920 F 9000.1000 material] -^ wash — > 



A, 15 mins. to 1 hr. 50°C. — > rinse — ♦ B, 15 mins. 50°C. — > quick rinse -+ C, 15 mins. 



50°C. — » B, till differentiated, room temperature — > i>, 2 mins. — > balsam via usual 



reagents 



22.21 Dufrenoy 1929 see DS 22.11 Milovidov 1928 (note) 



22.21 Fain and Wolfe 1939 see DS 11.421 Fain and Wolfe 1939 



22.21 Fieandt see DS 21.23 Fieandt (1933) 



22.21 Gough and Fulton 1929 see ADS 12.2 Gough and Fulton 1929 



22.21 Held test. 1900 Pollack Pollack 1900 



reagents required: A. water 100, acetic acid 0.1, erythrosin 0.7; B. water 50 DS 22.3 



Nissl 1898 47, acetone 3; C. 0.1% potassium alum 

 method: [sections] -^ water —» a, 2 mins. on slide while warming —» cool —> water, 



quick rinse — > B, on slide, warmed till no further smell of acetone -^ cool — > C, till 



sections turn reddish — > water, quick rinse —> blot — » neutral mountant, via propyl 



ale. 

 result: nuclei, red; nucleoli, nissl granules, mitochondria, blue; plasma, reddish. 



22.21 Hirsch and Bretschneider 1938 test. 1948 Romeis 



Romeis 1948, 233 

 reagents required: A. 3% ferric alum; B. 0.5% hematoxylin 



method: [sections of F 7000.1000 R6gaud fixed 1910 material] — * water ^^ A, }i-l hr. 

 60°C. — > quick wash — * B, 10-20 mins. 60°C. -^ thorough wash — » A, till differentiated 



22.21 Hollande 1930 6630, 104:473 



reagents required: A. DS 22.21 Altmann 1890, sol. A;B. 0.5% phosphomolybdic acid; 



C. 1% methylene blue 

 method: [5 M sections of F 3790.1000 Benoit 1922 fixed material, varnished on slide with 



5% collodion] —>■ water -^ A, 30 mins. -^ brief wash -^ B, 5 mins. —>■ C, 10-20 mins. 



-^ rinse -^ abs. ale, 1 min. — * amyl ale. till differentiated —> balsam, via xylene 



22.21 KuU 1913 see DS 13.22 KuU 1913 



22.21 KuU 1914 766, 45:153 



reagents required: A. DS 21.21 Altmann 1890, sol. A; B. 0.5% toluidine blue; C. 0.5% 



aurantia in 70% ale. 

 method: [sections of F 1670.0090 Champy 1913 fixed material] —> A, on slide, heated 



to steaming, 1 min. —y water, quick rinse —>■ B, 1-2 mins. — > water, quick rinse -^ C, 



till mitochondria differentiated — > abs. ale, minimum possible time — ♦ balsam, via 



xylene 

 note: This technique is often called Champy-Kull. Baker 1933 (p. 189) recommends 



fixation in F 3700.1000 Helly 1903, followed by "post-chroming" by the method of 



Parat 1926a below. A detailed description of the application of this technique is given 



under DS 22.20 above. 



22.21 McManns 1946 11431, 58:93 



reagents required: A. sat. sol. Sudan black in 70% ale. 



method: [sections of material fixed 1 month in F 8000.1000 McManns 1946] -^ 70% ale. 



— > A, 30 mins. -^ M 11.1 mountant 

 note: This also stains myelin and some fat granules. 



