DS 22.3 DYE STAINS OF SPECIAL APPLICATION 445 



22.3 Addison 1929 cit. compl. script. McClung 1929, 326 



foRxMUla: 1% thionin (or toluidine blue or methylene blue or cresyl violet) 

 method: [sections of material fixed in 5% acetic in 95% ale] — + stain, 6-12 hrs. — > 



water, quick rinse — » abs. ale. till differentiated — » balsam, via xylene 

 note: Spielmeyer 1924, 69, recommends a 0.1% dye solution, used hot. 



22.3 Anderson 1929 Anderson 1929, 42 



REAGENTS REQUIRED: A. 1% toluidine blue; B. ADS 21.1 Gothard 1898 

 method: [10 fx sections] — * water — > A, 10-20 mins., 50°C. —y wash -^ B, till differenti- 

 ated — > abs. ale. till dehydrated — > xylene — > [repeat B —y abs. — ♦ xylene, until re- 

 quired picture secured] — > cedar oil 



22.3 Bean 1926 20540b, 1 :56 



REAGENTS REQUIRED: A. either 1% neutral red or 1% methylene blue; B. either 0.5% 



methj'l orange or 0.5% eosin in 50% ale. 

 method: [12 jLi sections of F 0000.1010 Bean 1926 fixed material] -^ water — » A, 90°- 



iOO°C., in water bath, 20 mins. -^ 50% ale, till differentiated —> B, few sees. -^ 



balsam, via usual reagents 



22.3 Clark and Sperry 1945 20540b, 20 :23 



reagents required: A. 0.05% lithium carbonate; B. 0.25% thionin in 0.05% lithium 



carbonate 

 method: [celloidin sections] — * ^4, 5 mins. — > B, till grossly overstained -^ rinse -^80% 



ale, till differentiated -^ balsam, via butyl ale. and xylene 



22.3 Deipolli and Pomerri 1938 14425, 49:123 



REAGENTS REQUIRED: A. Water 100, DS 11.43 Ziehl 1882 2.5, acetic acid 0.5; B. water 



100, 40% formaldehyde 1, acetic acid 1 

 method: [sections of alcohol or formaldehyde-fixed material] — > waters A, 3-4 mins. 



-^ water, quick rinse -^ B, till Nissl bodies differentiated -^ water, wash — > balsam, 



via usual reagents 



22.3 Einarson 1932 608b, 8:295 



formula: water 100, chrome alum 5, gallocyanin 0.15 



preparation: Boil 20 minutes. Cool. Filter. 



method: [50 n celloidin sections of F 3700.0010 Zenker 1894 fixed material] —>■ water 

 — » stain, 12-24 hrs. -^ water, wash — > 80% ale, wash -^^ 95% ale, 1 hr. -^ abs. ale, 

 till dehydrated — » abs. ale. and ether, till celloidin removed -^ abs. ale, wash — » 

 — > balsam, via oil of Cretan thyme 



22.3 Einarson 1936 11135, 61:105 



formula: water 100, gallamin blue 0.2 

 preparation: Boil 5 minutes. Cool. Filter. 



method: [sections] —>■ water — » stain 12-24 hrs. —> water, wash -^50% ale, till differ- 

 entiated — > 95% ale, — » balsam, via usual reagents 



22.3 Gothard 1898 6630, 5 :530 



reagents required: 4. DS 11.44 Unna 1892; B. ADS 21.2 Gothard 1898 

 method: [celloidin sections of alcohol-fixed material]-^ A, 24 hrs. -^ 80% ale, quick 

 rinse -^ B, till differentiated — * balsam, via usual reagents 



22.3 Hansburg 1935 19938,81:364 



REAGENTS REQUIRED: A. DS 13.12 Wright 1910 100, DS 13.13 Giemsa 1902 20 

 method: [10 n paraffin sections] —^ water —»• A, on slide, 2 mins. — » dilute ^4 on slide to 

 3 times volume, leave 2 mins. — » wash, 1 min. — » 80% ale, 15 sees. — > abs. ale, least 

 possible time -^ balsam, via xylene 

 RECOMMENDED FOR: Nissl granules in spinal cord. 



22.3 Held 1900 see DS 22.21 Held 1900 



22.3 Huber test. 1943 Cowdry cit. Addison Cowdry 1943, 95 



REAGENTS REQUIRED: A. 0.1% toluidiuc bluc; B. 0.05% lithium c;irbonate 

 method: [sections of F 3000.0020 Huber (1943) fixed material]^ A, 15-18 hrs. -» 

 water, wash -^ B, 2 hrs. — * 70% ale, till differentiated — > balsam, via usual reagents 



