446 METHODS AND FORMULAS DS 22.3 



22.3 Johnson 1916 see DS 22.3 Kirkman 1932 (note) 



22.3 King 1910 763, 4:213 



formula: 1% phenol 100, thionin to sat. 



method: [sections] -^ stain, 2-3 mins. -^ water, quick rinses 95% ale, till differ- 

 entiated — > balsam, via usual reagents 



22.3 Keller 1945 4349,26:77 



REAGENTS REQUIRED: A. 0.5% cresyl violet; B. 50% Canada balsam in xylene 

 method: [sections] -^ A, 3-5 mins. —> wash — * xylene, via graded ales. -* B, 2 mins. — > 

 xylene — > [repeat abs. ale. — > J5 — » xylene cycle till differentiation complete] — > balsam 



22.3 Kirkman 1932 763, 51 :323 



formula: water 100, neutral red 0.2, acetic acid 0.4 

 method: [sections stained by DS 21.212 Weigert, or similar technique] -♦ water —> 



stain, 10-20 mins. -^ water, wash 95% ale, till differentiated-^ balsam, via usual 



reagents 

 note: The technique of Johnson 1916 (763, 11 :297) was essentially the same, with the 



additional provision that the neutral red be diluted from a 1 % solution which has been 



"ripened" 1-4 years. 



22.3 Lugaro 1905 19219,3:339 



reagents required: A. 0.05% toluidine blue; B. 4% ammonium molybdate 

 method: [sections of alcohol, or acetic alcohol, fixed material] — > A, 1-3 hrs. — » water, 

 rinse — » B, 15 mins. abs. ale. till differentiated — > balsam, via benzene 



22.3 Nissl 1894 15058, 13 :507 



reagents required: A. water 100, castile soap 0.2, methylene 0.4; B. 10% aniline in 

 95% ale. 



preparation of a: Dissolve soap in water. Add dye. "Ripen" some months. 



method: [sections of alcohol-fixed material] -^ stain, in watch glass, heated till bubbles 

 form — > B, in watch glass, till no more color comes away -* transfer to slide and blot 

 -^ oil of cajeput, till clear -^ balsam 



note: The original calls for venetianische Seife which was presumably the Sapo venetus 

 of the Austrian pharmacopeia of the period. This is identical in composition with the 

 sapo durus of the current pharmacopeia, commonly known as castile soap. Venetian 

 soap, specified by most texts, is a meaningless term in English. 



22.3 Roussy and Lehrmitte test. 1938 Carleton and Leach 



Carleton and Leach 1938, 243 

 REAGENTS REQUIRED: A. 1% thiouin, B. ADS 21.2 Gothard 1898 



method: [sections of formaldehyde-fixed material] -^ water -^ ^, 6 mins. -+ rinse —» 

 95% ale, 1 min. -^ B, till differentiated -* abs. ale. — > balsam, via xylene 



22.3 Sadorsky test. 1900 Pollack Pollack 1900, 100 



REAGENTS REQUIRED: A. 1% methylene blue ; B. 1% acetic acid 



method: [sections of formaldehyde-fixed material]^ A, overnight-^ B, till differenti- 

 ated 



22.3 Snider 1943 20540b, 18:53 



formula: water 15, 95% ale. 20, dioxane 65, toluidine blue 1 

 preparation: Dissolve dye in water and ale. Add dioxane. 



method: [1 cm. thick slabs of fresh brain] -^ stain, 3-5 days -^ [50 n sections by freezing 

 technique, fixed to slide with albumen] -^95% ale., till differentiated-^ balsam, via 

 usual reagents 



22.3 Spielmeyer 1924 see DS 22.3 Addison 1929 (note) 



22.3 Tsiminakis 1928 23632, 45 :50 



reagents required: A. 0.3% potassium permanganate; B. water 100, oxalic acid 0.5, 



sodium sulfite 0.5; C. 0.5% toluidine blue; D. 30% aniline in 95% ale. 

 method: [sections of chrome-fixed material] -> water -^ A, 5 mins. -> B, 10-15 mins. -» 



wash -^ C, 4 mins. -^ wash, 10 mins. —^ D, till differentiated -♦ balsam, via terpineol 



and xylene 



