DS 22.3-DS 22.4 DYE STAINS OF SPECIAL APPLICATION 447 



22.3 Windle, Rhines, and Rankin 1943 20540b, 18 :77 

 STOCK solutions: I. acetate-veronal buffer, pH 3.65. II. 1% thionin 

 WORKING solution: stock I 100, stock II 5 



method: [sections from formaldehyde or F 0000.0010 Carnoy 1887 fixed material]-* 

 water-* stain, 10-20 miiis. —> water, till no more color comes away — > 70% ale. till 

 no more color comes away -* balsam, via usual reagents 



22.4 Yolk and Fat Geanules 



Techniques for staining fat granules may be broadly divided into two classes. In the 

 first of these, fat-soluble stains such as Sudan II, or Sudan black B, are applied to sec- 

 tions obtained by the freezing technique, in which the fat is left in its original condition. 

 The other class consists of those in which fixation has taken place with a chromate- or 

 dichromate-formaldehyde mixture, with the result that certain of the unsaturated fatty 

 acids are rendered insoluble in the solvents used for paraffin sectioning, and may thus 

 be stained by almost any method which will bring out small objects. None of the tech- 

 niques given here are intended to be microchemical tests for the various classes of fat. 

 They are strictly histological methods to be used by those who desire to demonstrate 

 the existence of granules rather than to demonstrate the nature of the materials of 

 which the granules are composed. 



22.4 Arndt 1925 see DS 22.6 Arndt 1925 



22.4 Bacsich 1936 see DS 21.3 Bacsich 1936 



22.4 Bell 1914 11431, 19:105 



REAGENTS REQUIRED: DS 22.4 Daddi 1896 

 method: [paraffin sections of F 7000.0010 Tellyesniczky 1898 fixed material] ^70% ale. 



— > stain 10 mins. — » 50% ale. till differentiated -^ water ^^ Mil mountant (Chapter 



26) 

 note: Mulon 1914 (6593, 6:12) differs from above in specifying F 5000.1010 Bouin 1897 



fixation and DS 22.4 Herxheimer 1904 stain. 



22.4 Benda test. 1911 Mallory and Wright Mallory and Wright 1911, 386 



REAGENTS REQUIRED: A. Sat. sol. hematoxylin in 60% ale; B. ADS 21.1 Weigert 1885 

 method: [frozen sections of material fixed in F 4600.1010 Benda (1911)] -^ water -^ A, 

 6 hrs. -^ water, wash —>■ B, till differentiated — » [balsam, via usual reagents] or — > 

 water-* Mil mountant. 



22.4 Burden, Stokes, and Kimbrough 1942 see DS 23.219 Burdon, et al. 1942 



22.4 Clark 1947 11431, 59:337 



reagents required: A. 95% ale. 50, acetone 50, Sudan IV to sat.; B. DS 11.123 



Ehrlich 1886; C. 0.1% hydrochloric acid in 70% ale. 

 method: [sections by E 11.1 Clark 1947 (Chapter 27)]-* rinse -^ 70% ale. -^ A, 3-5 



mins.—* 70% ale, wash-* rinse—* B, 10 mins.—* wash -^ C, till differentiated—* 



tap water, till blue -^ M 12.2 Moore 1933 



22.4 Daddi 1896 1852, 26:143 



formula: 70% ale. 100, Sudan III to sat (circ. 0.2%) 

 method: [sections by freezing technique] — > water —> stain, 5-10 mins. — * water, wash 



— » Mil mountant (Chapter 26) 

 note: Rosenthal 1900 specifies fixation in his F 3000.1000 before this technique. 



22.4 Dietrich 1910 22264, 14 :263 



reagents required: A. 5% potassium dichromate; B. DS 11.113 Kultschitzky 1889; C. 



ADS 21.1 Weigert 1885 

 method: [frozen sections of formaldehyde-fixed material] —* il, 1-3 days, 37°C. — > 



water, wash -^ C, till differentiated (overnight) —» water, wash—* Mil mountant 



(Chapter 26) 



