448 METHODS AND FORMULAS DS 22.4 



22.4 Fischler 1904 23681, 16:913 



REAGPLVTs rp^quired: A. 12.5% nipric acetate; B. DS 21.212 Weigert 1885 (sol. C); 



C. ADS 21.1 Weigert 1885 5, water 95 

 method: [sections by freezing technique of formaldehyde-fixed material] — » water — > A, 



2-24 hrs. -* wash -^ B, 20 mins. -^ rinse — ► C, till differentiated -^ Mil or 12 moun- 



tant (Chapter 26) 



22.4 French 1940 1887a, 30:1243 



REAGENTS REQUIRED: A. 4% formaldehyde; B. water, 10 DS 21.13 Weigert 1898 50, sat. 



sol. Sudan III in equal parts acetone and 70% ale. 40; C. any DS 11.122 stain 

 method: [gelatin sections of formaldehyde-fi.xed material] -^ A, 1 day -^ wash -^70% 



ale, rinse— > A, 15 mins. — > 70% ale, rinse —> water, rinse —> C, 1-2 mins. — > wash 



-^ M 12.1 mountant 

 RECOMMENDED FOR: simultaneous demonstration of fat and elastic tissue. 



22.4 Galesesco and Bratiano 1928 6630, 99:1460 



PREPARATION OF STAINING SOLUTION: Digest 50 Gm. fragments of cortical zone of carrots 



in 100 ml. 95% ale. 2 hours on water bath. Cool. Digest in dark at room temperature 



8-10 days. Filter. Dilute to 80% ale. content. 

 REAGENTS REQUIRED: A. Staining solution from above; B. DS 11.122 Bohmer 1868 

 method: [frozen sections of formaldehyde-fixed material] — > 70% ale. -^ A, 6-24 hrs. —* 



70% ale. till no more color comes away ^^ water —> B, till nuclei blue — > wash — > 



M 12.1 mountant (Chapter 26) 



22.4 Gros 1930 23632, 47 :64 



reagents required: A. water 50, diacetin 50, scarlet R 0.6 

 preparation: Heat to 60°C. Cool. Filter. 



method: [frozen sections] —> water ^ A, 10 mins. 60°C. -^ wash -^ Mil mountant 

 (Chapter 26) 



22.4 Herxheimer 1901 23681, 14:891 



formula: 80% ale. 100, sodium hydroxide 2, Sudan IV 0.1 

 method: [sections by freezing technique of formaldehyde-fixed material] — >• 70% ale. —* 



stain, 2-4 mins. — > DS 11.122 counterstain, if desired — > M12 mountant (Chapter 26) 

 note: Herxheimer 1904 (23632, 21:57) substituted a mixture of equal parts 70% ale. 



and acetone for the alkaline alcohol; this is the more usually cited formula. In the 



place cited above he also recommends a sat. sol. indophenol (probably indophenol 



blue— Conn 1946, 87) in 70% ale. 



22.4 Herxheimer 1904 see DS 22.4 Herxheimer 1901 



22.4 Kionka 1894 764,1:414 



REAGENTS REQUIRED: .4. DS 11.22 Grenacher 1879; B. water 70, 95% ale. 30, orange G 



0.05, hydrochloric acid 0.1 

 method: [paraffin sections of ale. fixed chicken blastoderms] — > A, overnight—* quick 



rinse -^ B, till differentiated — > balsam, via usual reagents 

 recommended for: yolk granules in embryonic cells. 



22.4 Leach 1938 11431, 47:635 



REAGENTS REQUIRED: A. 50% diacetin; B. water 50, diacetin 50, Sudan black B to 



excess 

 PREPARATION OF B: Digest 2 days at 55°C. Cool. Filter. 



method: [sections by freezing technique of F 1670.0000 Zweibaum (1943) fixed mate- 

 rial] -+ A, 30 sees. -^ B, 2 hrs. -^ A, 30 sees. — > DS 11.21 counterstain, if desired — > 

 Mil or 12 mountant (Chapter 26) 



22.4 Lillie 1945 20540b, 20:7 



REAGENTS REQUIRED: A. sat. sol. oil blue N in 60% isopropyl ale. 85, water 15; B. 0.1 % 



Bismarck brown R; C. 5% acetic acid 

 method: [frozen sections of formaldehyde-fixed material] —> A, 5-10 mins. — ♦ water, 



rinse -^ C, till differentiated, 1 min. -^ water — > M12 mountant 

 NOTE : The dilution of A should be made immediately before use. 



