DS 22.4 DYE STAINS OF SPECIAL APPLICATION 449 



22.4 Lillie 1948a Lillie 1948, 159 



PREPARATION OF STOCK SOLUTION: Water 40, isopropyl ale. 60, oil blue N to sat. 

 REAGENTS REQUIRED: A. stock 60, water 30; 5. 0.1% Bismarck brown; C. 5% acetic acid 

 method: [frozen sections] -^ A, 5-10 mins. — > wash -^ B, 5 mins. — » rinse — > C, 1 min. 

 — > wash — » Mil mountant 



22.4 Lillie 1948b Lillie 1948, 159 



PREPARATION OF STOCK SOLUTION: A. isopropyl alc. 100, Sudan brown or oil red 4B or 



oil red O 1.5 

 REAGENTS REQUIRED: A. stock 60, Water 40; B. water 80, acetic acid 1.6, DS11.122 



Lillie 1941 20; C. 1% sodium phosphate, dibasic 

 method: [frozen sections] — > water— > A, 10 mins. —>■ wash — > 5, 5 mins. -+ rinse — > C, 



till blue — > wash -^ Mil or Iz mountant (Chapter 26) 



22.4 Lillie and Ashburn 1943 1780a, 36:432 



formula: sat. sol. Sudan IV in isopropyl alc. 50, water 50 



method: [frozen sections of formaldehyde-fixed material] —> water ^ A, 10 mins. — > 

 water, wash -^ DS 11.123 counterstain if desired -^ balsam, via usual reagents 



22.4 Martinotti 1914 23543, 91 :425 



reagents required: A. 1% chrysoidin; B. 1% potassium dichromate 

 method: [frozen sections of formaldehyde-fixed tissue]—^ water -^ A, some hrs. — > water, 

 rinse — > B, 1 min. — > water, wash — * balsam, via usual reagents 



22.4 McManus 1946 see DS 22.21 McManus 1946 



22.4 Mulon 1914 see DS 22.4 Bell 1914 (note) 



22.4 Peter 1904 see DS 11.25 Peter 1904 



22.4 Proescher 1927 20540b, 2 :60 



reagents required: A. 50% pyridine; B. water 30, pyridine 70, oil red 4; C. 60% 



pyridine 

 PREPARATION OF B: Add dye to mixed solvents. Leave 1 hour with occasional agitation. 



FUter. 

 method: [frozen sections of formaldehyde-fixed material]—* A, 3-5 mins. — * B, 3-5 



mins. -^ C, till differentiated -^ water —> Mil or 12 mountant (Chapter 26) 



22.4 Rosenthal 1900 see DS 22.4 Daddi 1896 (note) 



22.4 Schaffer 1926 test. 1933 Cajal and de Castro Cajal and de Castro 1933, 305 



reagents required: A. 5% potassium dichromate; B. DS 11.121 Weigert 1904; C. 



ADS 21.1 Pal 1887 (sol. B); D. sat. aq. sol. lithium carbonate; E. DS 12.221 van 



Giesen 1896 

 method: [paraffin sections of dichromate-fixed material] — > water — > /I, 1 wk., 37°C. -^ 



thorough wash^ B, 1-2 days, 37°C. -» wash -> C, till differentiated -^^ D, 37°C., 



till dark blue -^ thorough wash — » E, till sufficiently counterstained -^ balsam, via 



usual reagents 



22.4 Sheehan 1939 see DS 21.3 Sheehan 1939 



22.4 Sheehan and Storey 1947 see DS 21.3 Sheehan and Storey 1947 



22.4 Smith 1907 11431, 12:1 



reagents required: A. sat. sol. {circ. 0.2%) Nile blue; B. 1% acetic acid 

 method: [frozen sections of fresh or formaldehj'de-fixed material]—* A, 10-24 hrs. — > 

 water, wash — > Mil or 12 mountant 



22.4 Smith and Mair 1911 20214, 25:247 



reagents required: A. sat. sol. Nile blue 100, sulfuric acid 0.5; B. 2% acetic acid 

 preparation OF a: Boil under reflux 1-2 hours. Cool. Filter. 



method: [frozen sections of fresh tissue]—* water—* A, overnight—* B, till differenti- 

 ated -^ water, wash -^ M 1 1 mountant (Chapter 26) 

 result: fatty acid, blue. Neutral fats, red. 



