DS 22.6 DYE STAINS OF SPECIAL APPLICATION 451 



was that of Best 1906, and it has not yet been surpassed. One of the most satisfactory 

 methods for the differentiation of starch granules in plant tissues is that of Milovidov 

 1928, which is given in section 12.5 above for the reason that it is also intended for the 

 demonstration of the differentiation of the proi)lastids. An endeavor to adapt the 

 Feulgen 1924 technique to the demonstration of starch was made by Cr6tin 1941, and 

 has only the disadvantage that it requires mounting in liquid petrolatum witli the 

 subsequent difficulty of cementing the coverslip in place. In cases in which it is desired 

 to give general differential stain, as well as a stain for glycogen, the technique of Vas- 

 tarini-Cresi 1907 can be confidently recommended. 



22.6 Arndt 1925 23681,35:545 



REAGENTS REQUIRED: A. sat. sol. glucosc in 4% formaldehyde; B. sat. sol. glucose; C. 

 DS 22.6 Best 1906 (sol. A); D. DS 22.6 Best 1906 (sol. B);E. any DS 11.122 formula, 

 saturated with glucose; F. 70% ale. 50, acetone 50, chlorophyll to sat. 

 method: [pieces of fresh tissue] — » yi, 24 hrs. — > frozen sections — > B, till required — > C, 

 ^i-1 hr. — > D, till differentiated — > E, till nuclei stained — > B, thorough wash — > 70% 

 ale, 1 min. —y F, 15-20 mins. — > 70% ale, 1 min. -^ B, 1 min. —>■ levulose syrup 

 result: glycogen, red; fat, green. 



22.6 Bennhold 1922 14674, 2:1537 



reagents required: A. 1% Congo red; B. 1% lithium carbonate; C. any DS 11.122 



formula 

 method: [paraffin sections]^ water, thorough wash — » A, 15-30 mins. — » B, rinse —» 



80% ale, till color clouds cease — > wash — > [C, if counterstain required] — * balsam, via 



usual reagents 

 recommended for: amyloid. 



22.6 Bensley 1939 see DS 22.6 Best 1906 (note) 



22.6 Best 1906 1780, 23:520 



stock solution: water 75, potassium carbonate 1.2, carmine 2.5, ammonia 25 

 preparation of stock: Boil dye with salts 15 minutes. Cool. Filter. Add ammonia to 



filtrate. 

 reagents required: A. stock solution 25, ammonia 35, methanol 35; B. water 50, 95% 



ale. 40, methanol 20 

 method: [sections of alcohol-fixed material, stained by any DS 11.12 method] — » water 



— » A, overnight —> water, rinse — > B, till glycogen granules differentiated -^ balsam, 



via usual reagents 

 note: Bensley 1939 (20540b, 14:47) recommends pure methanol for B, with subsequent 



dehydration in acetone. See Neukirch 1909, below, for another way of using these 



solutions. Zieglwallner 1911 (test. Schmorl 1928, 218) stains fat in his F 1300.0010 



mixture before following Best's technique; Schmorl 1928, 218 recommends his F 



1600.0010 for the same purpose. 



22.6 Birch -Hirschf eld 1887 test. 1928 Schmorl Schmorl 1928, 210 



reagents required: A. sat. sol. Bismarck brown; B. 0.5% crystal violet; C. 1% acetic 



acid 

 method: [frozen sections] -^ A, 5 mins. -^95% ale, wash — > distilled water, wash -^ B, 



5 mins. — > C, till differentiated — > thorough wash -^ levulose syrup 

 recommended for: amyloid. 



22.6 Cretin 1941 6630, 135 :355 



reagents required: A. methanol 100, phenyl hydrazine 1, hydrochloric acid 1; B. DS 



11.43 Feulgen 1924; C. water 100, sodium bisulfite 2, hydrochloric acid 0.2 

 method: [sections of F 5000.0020 Cretin 1941 fixed materials] —* A, overnight — » B, few 



mins. — > C, several hrs. —> liquid petrolatum, via usual reagents 

 note: Solution A may also be u.sed to mordant the tissues before sectioning. 



22.6 Edens see DS 22.6 Schmorl 1928a (note) 



