452 METHODS AND FORMULAS DS 22.6 



22.6 Highman 1946a 1887a, 41 :559 



REAGENTS required: A. 0.5% Congo red in 50% ale; B. 0.2% potassium hydroxide in 



80% ale. 

 method: [sections of formaldehyde or ale. fixed material]-^ A, 1-5 mins. — » B, 1-3 



mins. till differentiated — »• wash — > eounterstain, if desired -^ balsam, via usual 



reagents 

 recommended for: demonstration of amyloid 



22.6 Highman 1946b 1887a, 41 :559 



REAGENTS REQUIRED: A. DS 11.121 Weigert 1904; B. 0.1% crystal violet in 2.5% acetic 



acid 

 method: [sections of formaldehyde or ale. fixed material] — > water -^ A, 5 mins. -^ wash 



-^ B, 1-30 rains. -^ wash ^ MS 11.1 Highman 1946 

 note: Lieb 1947 (591b, 17:413) substitutes a 0.3% solution of crystal violet in 0.3% 



hydrochloric acid for B, above. 

 recommended for: demonstration of amyloid. 



22.6 Klein 1906 590, 5 :323 



reagents required: A. sat. aq. sol. orange G 50, sat. aq. sol. acid fuchsin 50; B. sat. 



aq. sol. toluidine blue 

 method: [sections of F 3700.1000 Klein 1906 fixed material] — » water — > A, 1 min. wa.sh 



—* B, 1 min. — > wash -^ balsam, via usual reagents 

 result: chromatin and prozymogen, blue; zymogen, red. 

 recommended for: granules in Paneth cells. 



22.6 Krajian 1952 Krajian 1952, 169 



REAGENTS REQUIRED: A. DS 11.123 Harris 1900; B. water 80, glycerol 20, Congo red 



3.2; C. 2% sodium cyanide 

 method: [unmounted sections by freezing technique] -^ A, 15 sees. -^ tap water, wash 



— > B, 15 mins. — » wash -^ C, till differentiated, few sees. — » strand on slide and drain 



— + M 12.1 mountant 

 recommended for: amyloid. 

 note: Stated by author (loc. cit.) to be modification of DS 22. G Bennhold 1922. 



22.6 Langhans 1890 22575, 120:28 



reagents required: A. DS 11.22 Mayer 1881; B. ADS 12.2 Gram 1884; C. 2.5% iodine 



in abs. ale. 

 method: [eelloidin sections of ale. fixed material] — > A, 10-15 mins. — » B, 5-10 mins. — » 



— > C, till dehydrated -^ oil of origanum 

 recommended for: demonstration of amyloid. 



22.6 Lieb 1947 see DS 22.6 Highman 1946b (note) 



22.6 Lillie 1948 Lillie 1948, 143 



reagents required: A. water 100, nitric acid 0.5, sodium periodate 1; B. DS 11.432 



Lillie 1948; C. 0.52% sodium bisulfite; D. DS 11.121 Weigert 1904 

 method: [sections of formaldehyde-fixed material, varnished on slide with collodion] — » 

 water -^ A, 10 mins. -^ wash —> 5, 15 mins., with occasional agitation —> C, 3 

 changes, Vi mins. in each — > wash -^ D, 2-5 mins. — > tap water, till blue — > balsam, 

 via usual reagents 



22.6 Lubarsch test. 1905 Hall and Herxheimer Hall and Herxheimer 1905, 112 



formula: water 20, DS 11.122 Delafield (1885) 40, ADS 12.2 Gram 1884 40 

 method: [sections to water] — » stain, 5 mins. -^ abs. ale, least possible time for dehydra- 

 tion - > balsam, via xylene 

 recommended for: glycogen. 



22.6 Mayer test. 1938 Mallory Mallory 1938, 134 



REAGENTS required: .4. 0.5% crystal violet; B. 1% acetic acid; C. 7.5% potassium alum 

 method: [paraffin .sections, uHthoiil removal of paraffin] -"> A, 40°C., 5-10 mins. — > wash 

 -* B, 10-15 mins. -^ wash -> C, few minutes -^ wash -^ [fix to slide] -^ balsam, via 

 xylene 

 recommended for: demonstration of amyloid. 



