DS 22.6-DS 22.7 DYE STAINS OF SPECIAL APPLICATION 453 



22.6 Milovidov 1928 sre DS 22.5 Milovidov 1928 



22.6 Neukirch 1909 2^568 1 , 20 ::>;} 1 



REAGENTS REQUIRED: A. sat. sol. glucose ill sat. sol. mercuric chloride; li. sat. sol. glucose 



in 80% ale; C. ADS 11.1 Lugol; D. DS 22.6 Best 1906 (sol. A) E. DS 22.6 Best 1906 



(sol. B) 

 method: [pieces of fresh tissue] — > .4, 6-12 hrs. — > B, wash -^ (', 6 hrs. — > 95% ale, 24 



hrs. or till required --> cclloidiu sections — > water — > [hematoxylin nuclear stain, if 



required] — » D, overnight -^ rinse -^ B, till differentiated -^ balsam, via usual 



reagents 

 note: Neukirch {loc. cit.) also recommends glucose-saturated 40% formaldehyde in 



place of A, above, in which case steps B and C are omitted. Schmorl 1928, 216 finds 



this better for large pieces. 



22.6 Reilhes 1941 6630, 135:554 



formul.\: a. abs. ale. 100, iodine 8, ammonium iodide 2 



method: [paraffin sections of alcohol-fixed material]—* abs. ale. — > stain, dropped on 

 slide, several minutes — > abs. ale, quick rinse — » dry -^ Venice turpentine, direct 



22.6 Schmorl 1928a Schmorl 1928, 210 



reagents required: A. 0.5% crystal violet; B.\% acetic acid; C. 7% ammonium alum 

 method: [paraffin sections, or ribbons, not deparaffinized] — > A, floated on surface, 5-10 

 mins. 40°C. — > wash, floating on surface — » B, floating on surface, till differentiated 

 -^• wash, floating on surface — > C, floating on surface, till required — > wash, floating 

 on surface -^ strand on slide, dry with gentle heat —^ xylene till deparaffinized — » 

 balsam 

 recommended for: amyloid. 



note: Edens {test. Schmorl loc. cit., 211) uses 0.1% methyl violet in 0.3% hydrochloric 

 acid for 24 hours and omits steps B and C, above. 



22.6 Schmorl 1928b Schmorl 1928, 211 



reagents required: .4. DS 11.44 Unna 1891; B. 0.5% acetic acid; C. 7.5% ammonium 



alum. 

 method: [paraffin sections] -^ water — > A, 10-15 mins. -^ rinse — ♦ B, quick rinse — > C, 



2-5 mins. — > abs. ale, minimum possible time —> balsam, via xylene 

 recommended for: amyloid. 



22.6 Schmorl 1928c see DS 22.6 Best 1906 (note) 



22.6 Schmorl 1928d Schmorl 1928, 209 



reagents required: A. \% methyl violet; B. 2% acetic acid 



method: [frozen sections] -^ A, }^-l min. — > B, 2-3 mins. — > thorough wash — » glycerol 

 recommended for: amyloid. 



22.6 Vastarini-Cresi 1907 test. 1942 Langeron Langeron 1942, 1173 



reagents required: A. DS 21.13 Weigert 1898 (working solution) 150, resorcin 6, 



cresofuchsin 3, 95% ale 150, hydrochloric acid 6; S 1% orange G in 95% ale 

 method: [paraffin sections] -^95% ale — > A, 2 hrs. 35°C. — > 95% ale, thorough wash 



—* B, I min. -^ balsam, via usual reagents 

 result: elastic fibers, blue; glycogen, red; plasma, pale orange. 



22.6 Zieglwallner 1911 see DS 22.6 Best 1906 (note) 



22.7 Mucin 



22.7 Highman 1945 20540b, 20 :85 

 formula: water 75, 95% ale 25, new methylene blue N 0.1, citric acid 0.2 

 method: [sections] —> water —» stain, 3-10 mins. ^ rinse —> balsam, via acetone and 



xylene 



22.7 Hoyer 1900 1780, 36:310 



REAGENTS REQUIRED: A. sat. aq. sol. mercuric chloride; B. 0.005% thionin 

 method: [sections of mercuric chloride-fLxed material] —> water—* .4, 30 sees. — > 95% 

 ale, wash -^ B, 5-15 mins. — > balsam, via S 22.1 Minot (1928) 



