454 METHODS AND FORMULAS DS 22.7-DS 22.8 



22.7 Lillie 1928 23632, 45 :381 



REAGENTS REQUIRED: 0.2% toluidine blue 

 method: [sections]—* waters stain, 1 min. — > quick rinse -^ acetone, till dehydrated 



-^ balsam, via xylene 

 note: This method was republished by Lillie 1929 (4349, 12:120) to which later paper 



reference is most frequently made. 



22.7 Mallory 1938 Mallory 1938, 131 



REAGENTS REQUIRED: A. DS 11.122 Mallory 1938, 10, 95% ale. 9, tap water 81; B. 



0.1% magenta 

 method: [paraffin sections alcohol-fixed material] -^ water -^ A, 5-15 mins. -^ thorough 



wash -^ B, 5-10 mins. — > 95% ale, till no more color comes away —> balsam, via 



xylene 



22.7 Masson 1910 test. 1948 Romeis Romeis 1948, 481 



REAGENTS REQUIRED: A. Any DS 11.122 formula; B. 5% metanil yellow in 0.2% acetic 



acid; C. DS 22.7 Mayer 1896a (working sol.) 

 method: [sections of F 5000.1010 Bouin 1896 fixed material]—* water -^ A, till deeply 



stained — > rinse — > B, till nuclei differentiated -^ wash —>■ C, 5-10 mins. -^ wash — > 



balsam, via usual reagents 



22.7 Mayer 1896a rnucihaematin — compl. script. 14246, 12 :303 



REAGENTS REQUIRED: A. water 30, 95% ale, 70, hematin 0.2, aluminum chloride 0.1, 



nitric acid 0.05 

 method: [sections of alcohol-fixed material] — > water -^ A, 10 mins. —* 1 hr. — > thorough 



wash — > balsam, via usual reagents 

 note: Mallory 1938, 129 recommends this for mucin of fibroblastic origin. 



22.7 Mayer 1896b mucicarmine — compl. script. 14246, 12 :303 



STOCK solution: Heat together for 2 minutes water 2, aluminum chloride 0.5, carmine 1. 



Add with constant stirring 100 50% ale. 

 working solution: stock 10, 70% ale. 90 

 method: [sections of alcohol-fi:xed material] —> stain, 10-15 mins. -^ rinse -^ balsam, 



via usual reagents 

 note: Mallory 1938, 130 recommends this for mucin derived from epithelial cells. 



22.7 Merkel test. 1928 Schmorl Schmorl 1928, 164 



REAGENTS REQUIRED: A. 5% cresyl violet; B. abs. ale. 50, toluene 50 

 method: [5 m-7 m sections of alcohol-fixed material]^ A, 10-30 mins. -+ 95% ale, 

 rinse —* B, controlled under microscope, till differentiated — > balsam, via xylene 



22.7 Unna 1894 14352, 18 :509 



REAGENTS REQUIRED: A. DS 11.44 Unna 1892; B. 0.1% acetic acid; C. 10% potassium 



dichromate; D. 1% hydrochloric acid in aniline 

 method: [sections of alcohol-fixed material]-^ water—* A, 10 mins. -^ B, rinse—* C, 



30 sees. — > blot —>■ D, till differentiated, 15-30 sees. -^ balsam, via usual reagents 



22.7 Zimmerman 1925 see DS 21.4 Zimmermann 1925 



22.8 Other Cell Inclusions and Extrusions 



22.8 Alzheimer 1910 Nissl and Alzheimer 1910, 411 

 REAGENTS REQUIRED: A. sat. aq. sol. copper acetate; B. DS 11.124 Alzheimer 1910 

 method: [sections prepared as in steps A, and B (DS 21.213 Alzheimer 1910)] — * water 



-^ ^, 1 hr. 35°C. — * rinse — * B, 30 mins. — * wash — * balsam, via usual reagents 

 recommended for: demonstration of granules in nerve cell cytoplasm. 



22.8 Bensley test. 1915 Chamberlain Chamberlain 1915, 134 



reagents required: A. ADS 12.1 Gram 1884; B. sat. aq. sol. copper acetate; C. 0.5% 



hematoxylin; D. 5% potassium dichromate; E. ADS 21.1 Weigert 1885 

 method: [sections of F 3700.1000 Bensley (1915) fixed material]-^ water -^ A, some 

 hrs. — > wash -^ B, 5-10 mins. — * wash, 1 min. — * C, 5-10 mins. -^ wash, 1 min. -^ D, 

 30 sees. — * E, till differentiated — * wash — > balsam, via usual reagents 

 recommended for: canaliculi in plant cells. 



