DS 22.8 DYE STAINS OF SPECIAL APPLICATION 455 



22.8 Beyard 1930 4285a, 7 :264 



REAGENTS REQUIRED: A. 1% acid fuchsin; B. 0.8% bromine 



method: [sections] -^ water — > A, 30 sees. — > rinse —>■ B, 4-5 sees, till violet — * 95% ale., 

 till pale mauve —y [DS 11.111 nuclear stain if required] -^ balsam, via usual reagents 

 recommended for: basophilic granules in mast cells. 



22.8 Bowie 1935 763, 64:357 



PREPARATION OF DRY STAIN: Add 67 0.4% ethyl violet to 33 0.4% Biebrich scarlet. 



Collect, wash and dry ppt. 

 STOCK SOLUTION: 1% dry stock in 95% ale. 

 REAGENTS REQUIRED: A. 95% alc. 80, Water 20, stock sol. 0.1-0.5; B. 50:50 clove 



oil: xylene 

 method: [sections of F 7000.1000 Regaud 1910 material] -^ water -> A, 24 hrs. -> blot 



— » B, till differentiated, 20-30 mins. — > balsam, via benzene 

 RECOMMENDED FOR: pepsinogen granules in gastric mucosa. 



22.8 Brice 1930 20540b, 5:101 



REAGENTS REQUIRED: A. abs. alc. 100, benzidine 0.3, sodium nitroferricyanide 0.4; B. 



water 100, hydrogen peroxide 0.5; C. DS 13.12 Wright 1910, working solution 

 method: [air-dried blood smear] — > A, flooded on slide, 1 min. -^ B, equal in quantity 

 to A, flooded on slide, 3 mins. -^ water, thorough wash — * blot — > dry — > C, flooded 

 on slide, 2 mins. — * add water, drop by drop, till green scum forms on surface, leave 

 2 mins. — > water, thorough wash -^ [dry] or neutral mountant, via acetone 

 recommended for: oxidase granules in erythrocytes. 



22.8 Bujard 1930 4285a, 7 :264 



reagents required: A. 1% acid fuchsin; B. 0.8% bromine; C. 1% hydrochloric acid 



in 95% alc. 

 method: [sections] -^ water —> 4, 30 sees. — » j5, 4-5 mins. — > C, till granules alone 



stained — > DS 11.111 Regaud 1910 if nuclear staining is required — > balsam, via usual 



reagents 

 recommended for: basophilic granules in mast cells. 



22.8 Ehrlich 1891 test. 1938 Mallory Mallory 1938, 174 



formula: water 30, 95% alc. 62, acetic acid 8, Hofmann's violet to sat. 

 recommended for: granules in mast cells. 



22.8 Hall and Powell 1926 21400a, 45:256 



reagents required: A. 0.2% Bordeaux red; B. 4% ferric alum; C. 0.5% hematoxylin 

 method: [flagellates fixed in F 3000.0000 Schaudinn 1893]-^ water—* A, 1-2 days— » 



B, 1-2 days — > C, 1-2 days — > B, till differentiated —>■ balsam, via usual reagents 

 recommended for: internal structures in euglenoid flagellates. 



22.8 Harvey 1907 590, 6 :207 



REAGENTS REQUIRED: A. sat. aq. sol. (circ. 7%) cupric acetate; B. 3% potassium di- 



chromate; C. sat. aq. sol. hematoxylin; D. ADS 21.1 Weigert 1885 

 method: [sections of F 3700.1000 Harvey 1907 fixed material] -^ A, 1 min. -^ wash — > 



B, 1 min. — > wash — ♦ C, 1 min. — > wash — > D, till differentiated — » balsam, via usual 



reagents 

 RECOMMENDED FOR: demonstration of parietal cell granules. 



22.8 Hamperl 1926 22575, 259:179 



REAGENTS required: A. Any DS 11.21 formula; B. 0.01% methyl violet 



method: [sections of F 0000.1000 Hamperl 1926 fixed material] —> water -^ A, till 



nuclei stained —^ wash — > B, till granules stained -^ abs. alc. least possible time — » 



balsam, via usual reagents 

 recommended for: granules in chief cells of stomach. 



22.8 Heidenhain 1894 1780, 42 :665 



reagents required: A. 0.01% aniline blue; B. DS 11.111 Heidenhain 1892 (both sol.) 

 method: [sections of F 3000.0010 material] —> A, 24 hrs. — > B, full technique -^ balsam, 



via usual reagents 

 recommended for: centrosomes in animal tissues. 



