456 METHODS AND FORMULAS DS 22.8 



22.8 Horvath 1931 23632, 47 :463 



REAGENTS REQUIRED: A. 1% phosphotungstic acitl; B. 0.1% toluidine blue 

 method: [protozoa, fixed and mordanted 20 mins. in sat. aq. sol. mercuric chloride] — + 

 water, thorough wash -^ A, 10 mins. —>■ water, rinse -^ B, 1 min., 50°-60°C. — > bal- 

 sam, via usual reagents 

 recommended for: demonstration of cilia, cirri, and basal bodies in ciliate protozoa. 



22.8 Kraus 1914 22575, 218:107 



reagents required: A. DS 11.44 Unna 1892; B. 25% tannin; C. DS 12.16 Fraenkel 



(1948); D. 2% acid fuchsin; E. 1% phosphomolybdic acid 

 method: [3 iU-5 m sections of formaldehyde-fixed material]-^ waters A, 6 mins.—* 

 wash —> B, till no more color comes away —> C, till nuclei blue — ♦ wash —> D, I min. 

 -^ rinse — * E, 30 sees. — > wash -^ [drain and lalot] — ^ abs. ale. least possible time — > 

 balsam, via xylene 

 recommended for: differentiation of tannic acid fast (red-violet), fuchsinophile (red- 

 dish yellow) and fuchsinophojje (blue) colloid in thyroid. 



22.8 Lendrum 1945 11431, 57:267 



REAGENTS REQUIRED: .4. DS 11.122 Mayer 1901; B. 1% fast green FCF in 0.5% acetic 



C. ADS 12.2 Lugol; D. 2% phosphotungstic acid in 95% ale; E. DS 11.46 Lendrum 



1945 

 method: [sections] —>■ A, till thoroughly stained — » wash — > B, 1-2 mins. — * rinse -^ C, 



2 mins. -^^ 95% ale, rinse —> D, few moments —> rinse—* E, 2-6 mins. — > wash—* 



balsam, via usual reagents 

 RECOMMENDED FOR: granules in cystic breast epithelium and basement membrane of 



renal glomerulus. 



22.8 MacCallum et al. see DS 21.42 MacCallum et al, 1935 



22.8 Mallory 1938a Mallory 1938, 136 



REAGENTS REQUIRED: A. 0.5% magenta in 50% ale. 

 method: [sections with nuclei prior stained by any DS 11.122 method] —> water — ' A. 



5-20 mins. -^95% ale, till differentiated -> balsam, via xjdene 

 RECOMMENDED FOR: demonstration of hemafuchsin granules. 



22.8 Mallory 1938b Mallory 1938, 151 



REAGENTS REQUIRED: A. 1% acid fuchsin; B. 0.1% potassium permanganate 

 method: [sections from F 3700.0010 Zenker 1894 fixed material]—* water—* A, over- 

 night — > drain — * B, 40-60 sees. -^ abs. ale, — * balsam, via xylene 

 result: fibroglia fibrils, bright red; collagen, reddish yellow; elastic fibrils, bright yellow. 

 recommended for: demonstration of fibroglia fibrils. 



22.8 Mallory 1898 test. 1938 Mallory Mallory 1938, 138 



REAGENTS REQUIRED: A. 95% alc. 75, sat. sol. ammonium sulfide 25; B. water 100, 



hydrochloric acid 0.5, potassium ferricyanide 10; C. 0.5% magenta in 50% ale 

 method: [sections] -^ water — > A, 1-24 hrs. — * wash — * B, 10-20 mins. — > wash — > C, 



5-20 mins. -^ rinse -^95% ale, till differentiated — * balsam, via usual reagents 

 result: nuclei and hemofuchsin, red; hemosiderin, blue. 

 RECOMMENDED FOR: differentiation of hemosiderin and hemofuchsin in sections. 



22.8 Mallory 1938 Mallory 1938, 207 



REAGENTS REQUIRED: A. DS 11.122 Mallory 1938; B. 0.5% phloxine in 20% ale; C. 



0.1% lithium carbonate 

 method: [sections of alc. or formol fixed material] — > A, 1-5 mins. -^ tap water, wash — > 

 B, 10-30 mins. — * tap water, wash -^ C, J^-1 min. -^ tap water, wash — » balsam, via 

 usual reagents 



RECOMMENDED FOR! hyaliu. 



22.8 Mcjunkin 1922 763, 24 :67 



REAGENTS REQUIRED: A. watcr 25, 95% alc. 75, benzidine 0.2, hydrogen peroxide 0.2; 



B. Any DS 11.122 solution; C. 0.1% eosin 

 method: [5 n sections of formaldehyde-fixed material] -^ water — > A, 5 mins. -^ wash -^ 



B, 2 mins. -^ wash — * C, 20 sees. —> wash — > balsam, via usual reagents 

 RECOMMENDED FOR: peroxidase granules in bone marrow. 



