DS 22.8 DYE STAINS OF SPECIAL APPLICATION 457 



22.8 Oliver 1934 11250, 65:266 



REAGENTS REQiriREi): A. ADS 12.2 Olivcr 1934; B. DS 11.13 Ziehl 1882 



method: [air-dried smear from a 1 : 4 dilution of blood in hirudin saline, incubated 40-50 



mins. 37°C.] -^ A, 20 mins. -> wash -^ blot -» B, 20 mins. —>■ wash -> dry 

 RECOMMENDED FOR: " flagella " on erythrocytes. 



22.8 Oppler test. 1928 Schmorl Schmorl 1928, 341 



REAGENTS REQUIRED: .1. DS 11.24 Oppler (1928); B. 0.5% picric acid in 95% ale. 

 method: [celloidin sections of alcohol-fixed material]-* water-* A, \i-l min. -» blot 



-^ B, \ min. — + balsam, via usual reagents 

 recommended for: demonstration of eleidin in cells of stratum lucidum of skin. 



22.8 Reich test. 1933a Cajal and de Castro Cajal and de Castro 1933, 323 



reagents required: A. 1% toluidine blue 

 method: [15 At celloidin sections of F 7000.0000-Muller 1890 fixed nerve] -> water -^ A, 



5 mins. -> quick rinse -^ 95% ale. till differentiated-* abs. ale, till no more color 



comes away — > balsam, via usual reagents 

 recommended for: granules in Schwann cells. 



22.8 Reich test. 1933b Cajal and de Castro Cajal and de Castro 1933, 324 



REAGENTS REQUIRED: A. water 100, phenol 5, acid fuchsin 1; B. ADS 21.1 Pal 1887 (A 



and B sols.); C. 2% toluidine blue 

 method: [15 M frozen sections of formaldehyde-fixed nerve] -^ A, 24 hrs. 37°C. — > B 



{A sol.), 30 sees. -» B, (5 sol.), few moments -* [repeat B (A) -* B (B) cycle till no 



more color comes away] —> balsam, via xylene 

 results: nuclei, blue; n granules, red; w granules, purple. 

 RECOMMENDED FOR: differentiation of n and tt granules in Schwann cells. 



22.8 Russel test. 1894 Kahlden and Laurent Kahlden and Laurent 1894, 73 



REAGENTS REQUIRED : A . sat. sol. magenta in 2 % phenol ;B.1% iodine green in 2 % phenol 

 method: [sections of dichromate-fixed material]^ A, 10 mins. ^ thorough wash -> 



abs. ale, 30 sees. -^ B, 5 mins. -* balsam, via usual reagents 

 RECOMMENDED FOR: miscellaneous cellular inclusions. 



22.8 Russell test. 1928 Schmorl cil. Miller Schmorl 1928, 219 



REAGENTS REQUIRED: A. DS 11.43 Ziehl 1882; B. water 100, phenolS, iodine green 1 

 method: [sections]-^ water-* a, 10-30 mins. -^ wash -* abs. ale. Ja-l min. ^ B 



5 mins. ~y abs. ale, wash —> balsam, via usual reagents 

 RECOMMENDED FOR: hyalin. 



22.8 Russell test. 1948 Lillie cit. Ehrlich Lillie 1948, 105 



REAGENTS REQUIRED: A. sat. sol. magenta in 2% phenol; B. 1 % iodine green in 2% phenol 

 method: [sections of F 7000.0000 Miiller 1850]-* waters A, 10-30 mins. -* wash, 



5 mins. -^ abs. ale, 30 sees. -* 5, 5 mins. -^ abs. ale, till differentiated -* balsam, via 



usual reagents 

 results: nuclei, green; Russell's bodies, red. 

 recommended for: demonstration of Russell's bodies. 



22.8 Sannomiya 1927 8542a, 5 :202 



REAGENTS REQUIRED: A. 10% hydrochloric acid; B. 1% acid fuchsin; C. sat. sol. picric 



acid; D. 1% phosphomolybdic acid. 

 method: [small pieces fresh pancreas] -* A, 24 hrs. -^ running water, 24 hrs. -^ celloidin 



sections -* 5, 3 mins. -^ water, thorough wash -* C, 3-4 mins. -^ water, quick rinse 



-^ D, 1-2 mins. -^ water, rinse -* balsam, via usual reagents 

 results: paranuclei, red. 



recommended for: demonstration of paranuclear bodies in pancreas. 

 22.8 Schmorl 1928 Schmorl 1928, 244 



REAGENTS REQUIRED: A. DS 22.21 Altmann 1890 (sol. A); B. DS 22.21 Altmann 1890 



(sol. B) 



method: Jblood smears fixed for 1 hr. in 1% osmic acid] ^ quick wash-* A, 5 mins., 

 55°-60°C. -* wash, till no more color comes away -* Ji, 15 mins. —* rinse —* blot — * 

 abs. ale least possible time — > balsam, via xylene 



recommended for: Schridde's granules in lymphocytes. 



