DS 23.13 DYE STAINS OF SPECIAL APPLICATION 465 



23.13 Dawson 1934 11284, 20:G59 



REAGENTS kequired: . t . 2 '^ pliloxiiic; B. DS 11.44 Loffler 1890 

 method: [methaiiol-fixed smears] -^ water -» A, 2-5 mins. — > water, wash — » li, 15 sees. 



-^ 20% ale, till no more color comes away — > balsam, via usual reagents 

 note: a detailed descrii)tiou of the use of this technique is given on p. 75. 



23.13 Frotheringham test. 1952 Krajian and Gradwohl Krajian and Gradwohl 1952, 216 

 kokmula: water 100, sat. aq. sol. magenta 0.9, sat. aq. sol. methylene blue 0.6 

 method: [smears from hippocampus or cerebellum (see Chapter 8)]— » abs. ale., few 



moments — + stain, 10-15 sees. -^ blot -^ dry 

 result: Negri bodies red with blue granules. 



23.13 Gallego 1923 23454,25:74 



REAGExNTs REQUIRED: A. watcr 100, ferric chloride 0.3, nitric acid 0.3; B. water 100, 



DS 11.43 Ziehl 1882 3, acetic acid 0.3; C. water 100, 40% formaldehyde 0.6, nitric acid 



0.3; D. DS 12.211 Cajal 1895 

 method: [sections by freezing technique of F 0000.1010 fixed material] -^ A, 1-5 mins. — » 



B, poured on slide, 5 mins. -^ water, wash -+ C, 1-10 mins. -> wash -^ D, I min. — > 



— > balsam, via usual reagents 



23.13 Gerlach 1926 test. 1948 Lillie Lillie 1948, 226 



REAGENTS REQUIRED: A. Water 100, DS 11.44 Loffler 1890 12, DS 11.43 Ziehl 1890 6 

 method: [paraffin sections of alcohol-fixed material]-^ A, poured on slide, heated to 

 steaming, 1 min. -^ repeat 3 further times —> neutral mountant, via usual reagents 



23.13 Goodpasture 1925 608b, 1:550 



REAGENTS REQUIRED: A. Water 80, alcohol 20, phenol 1, anihne 1, magenta 0.5; B. DS 



11.43 Loffler 1890 

 method: [thin sections of F 3700.0010 Zenker 1894 material] -♦ water -^ A. 10-20 mins, 



-* wash -^ blot -^ 95% ale, till light pink -> wash — > 8-15 sees, to 1 min. -> abs. ale. 



till differentiated — > balsam, via xylene 

 result: Negri bodies red on blue. 



23.13 Hamilton 1934 11587, 37:139 



REAGENTS REQUIRED: A. water 50, 95% ale. 50, ethyl eosin 0.5, eosin Y 0.5; B. sat. sol. 



potassium alum; C. 0.15% ammonia in 95%, ale.; D. 0.5% methyl blue 

 method: [4 11-6 n sections of F 3700.1000 Helly 1903 fixed material] -^ A, on slide, set 

 ahght and allowed to burn out -* repeat -^ drain -^ B, flooded on slide -^ drain -> B, 

 in jar, 10 mins. -^ wash -^ C, tiU tissue pink —>■ wash -^ D, 10 mins. -^ abs. ale, rinse 

 -^ C, till inclusion bodies (vermilion) clearly differentiated — > wash -♦ balsam, via 

 usual reagents 

 RECOMMENDED FOR: virus inclusion bodies in general. 



23.13 Harris 1908 11250, 5:566 



reagents required: a. 1%, ethyl eosin in 95% ale; B. water 80, DS 11.44 Unna 1892 20 

 method: [alcohol-fixed smears] -^ water —> A, 1-3 mins. —» water, quick rinse ^ B, 

 10 sees. -* water, rinse -^95% ale, tfll differentiated — > balsam, via usual reagents 



23.13 Jordan and Heather 1929 20540b, 4:121 



reagents required: A. ADS 12.2 Lugol (1905); B. 2% sodium thiosulfate; C. water 



100, eosin Y 0.5, phloxine 0.25; D. 0.1% azur B; E. clove oil 30, abs. ale 60 

 method: [5 M sections by E 22.1 Jordan and Heather 1929 method of F 3800.1000 

 Dominici 1905 fixed tissues] — > A, 10 mins. — > wash -^ B, till decolorized —>■ thorough 

 wash-* C, 15 mins. -^ wash-* D, 2-5 mins. -» 95% ale, wash -> E, till differenti- 

 ated -^ abs. ale, least possible time — ♦ xylene — > gum elemi 



23.13 Kaiser and Gherardini 1934 23684, 131:128 



reagents required: A. water 100, phloxine 0.5, aniline 1.5 

 method: [sections] — * water — > A, 1 min. -^ wash — > balsam, via usual reagents 

 recommended for: Guarnieri bodies 



23.13 Krajian 1941 see DS 23.221 Krajian 1942 



