DS 23.13-DS 23.2 DYE STAINS OF SPECIAL APPLICATION 467 



23.13 Petragnani 1928b test. 1930 Ciferro 20540b, 6:35 



REAGENTS REQUIRED: A. ADS 12.2 Petragnaiii (1928) (working sol.); H- 0.5% acid 



fuchsin in 50% ale. 

 method: [sections] — + abs. ale. -^ A, few sees. -^ abs. ale., rinse ^70% ale, rinse — » B, 



30 sees. — > wash — » balsam, via usual reagents 



23.13 Parsons 1939 4349, 19:104 



REAGENTS REQUIRED: A. water 94, 95% ale. 5.4, acetic acid 0.6, ethyl eosin 0.054; B. DS 



11.44 Jadassohn (1928); C. 0.025% acetic acid 

 method: [paraffin sections of formaldehyde-fixed material] —> water—* A, 2 mins. -^ 



95% ale., till pink — * B, 2 mins. -^ rinse -+ C, till pale bluish pink — > 95% ale, rinse 



— > abs. ale, least possible time -^ balsam, via xylene 

 result: Negri bodies, bright orange red with blue central dot; Nissl bodies, blue. 



23.13 Rich 1948 see DS 23.13 McWhorter 1941 (note) 



23.13 Schleifstein 1937 617, 27:1283 



STOCK solution: methanol 50, glycerol 50, magenta 0.9, methylene blue 0.5 

 WORKING solution: stock 1.25, 0.0025% potassium hydroxide 100 

 method: [paraffin sections of F 3700.0010 Zenker 1894 fixed material] — * water —* stain, 

 poured on slide, warmed to steaming, 5 mins. — » 90% ale, till pale violet -^ balsam, 

 via usual reagents 



23.13 Stovall and Black 1940 591b, 10:1 



REAGENTS REQUIRED: A. 1% ethyl eosin in pH 3.0 buffer; B. 0.25% methylene blue in 



50% ale at pH 5.5; C. 0.4% acetic acid 

 method: [acetone-fixed material] -^ A, 2 mins. -^ water, rinse -^ B, 30 sees. — > C, till 



brownish red -^ water, wash — >• balsam, via usual reagents 

 note: a detailed description of the use of this technique is given under DS 23.10 above _ 



23.13 Taniguchi, Hosokawa, Kuga, Komora, and Nakamura 1932 test. 1933 Findlay 



11360, 53:43 

 REAGENTS REQUIRED: A. acetone; B. 1% cadmium iodide in 40% formaldehyde; C. 



water 92.5, 95% ale 5, 40% formaldehyde 2.5, eosin 0.1; D. DS 11.43 Ziehl 1882 

 method: [air-dried smear] -^ A, 1 min. — > wash -^ B, 2 mins. — * rinse -^ C, 30 sees. — > 



rinse -^ D, 10 sees. -^ wash -^ dry 

 note: Any turbidity in B is cleared up with a few drops of hydroclilorie acid. 



23.13 Turewitsch 23684, 129:381 



REAGENTS REQUIRED: A. 2.5% ferric alum; B. 5% tannic acid; C. 0.02% azur I; D. sat. 



sol. picric acid 

 method: [sections] -^ A, 20-25 mins. — * wash — > B, 10 mins. -^ wash — » D, 10-15 mins. 



-^ wash -^ balsam, via usual reagents 

 recommended for: Pasehen bodies in cornea. 



23.13 Williams 1908 618, 18:10 



formula: sat. ale sol. (circ. 6%) magenta 1.25, sat. ale. sol. (circ. 3.5%) methylene blue 



25, water 75 

 method: [smears] -^ cover with stain, heat to steaming — > wash -^ blot — » dry 



23.13 Zottner 1934 6630, 115:593 



reagents required: A. 30% nitric acid; B. DS 11.43 Ziehl 1882; C. water 100, picric 



acid 0.5, indigocarmine 0.5 

 method: [paraffin sections]^' abs. ale — > A, few drops on slide, 2-3 mins. -^ B, on 



slide, 15 sees. —>■ rinse — > C, 15 sees. -^ abs. ale, till green -^ balsam, via xylene 



23.2 Bacteria gether of the techniques given below as 



Bacteria can be stained by any of the "bacterial stains" is due entirely to the 



techniques which are customarily applied fact that persons skilled in the staining of 



to nuclei, as well as the majority of tech- zoological and botanical forms have 



niques which are used for the demonstra- drifted so far from bacteriological tech- 



tion of mitochondria. The grouping to- nicians as to tend to throw these bacterio- 



