DS 23.212 DYE STAINS OF SPECIAL APPLICATION 475 



method: [dried smears] -^ A, dropped on slide, 2 mins. — » quick rinse — > B, 1 min. — > 



quick rinse — > C, till differentiated —^ water, to stop differentiation — > dry — * balsam 



note: a detailed description of the use of this technique is given under DS 23.210 above. 



23.212 Konschegg 1940 14674, 87 :465 



REAGENTS REQUIRED: A. watcr 90, 95% ale. 7, aniline 3, gentian violet 2; B. sat. aq. 



sol. picric acid 

 method: [heat-fixed smears] —* A, 5 sees. -^ B, 5 sees. — > 95% ale, till no more color 



comes away -^ dry 

 note: This stain, though not using iodine, is intended to demonstrate Gram-positive 



bacteria. 



23.212 Kopeloff and Beerman 1822 11250, 31:480 



reagents required: A. water 105, crystal violet 0.75, sodium bicarbonate 1.25; B. ADS 



12.1 Atkins 1920; C. ether 25, acetone 75; D. 0.1% magenta 



method: [smears] — > A, 5-10 mins. — » B, rinse —^ B, fresh solution, 2 mins. -^ blot — > 

 C, dropped on slide, till no more color comes away —> dry — ♦ D, 5-10 sees. — > water, 

 wash -^ dry 



result: Gram-positive, blue; Gram-negative, red. 



23.212 Kopeloff and Cohen 1928 20540b, 3 :64 



REAGENTS REQUIRED: A. 1% methyl violet 6B 60, 5% sodium bicarbonate 8; B. ADS 



12.2 Kopeloff and Cohen 1928; C. acetone 50, 95% ale. 50; D. 0.1% magenta 

 method: [heat-fixed smear] -^ A, on slide, 5 mins. — > B, on slide, 2 mins. -^ drain -+ C, 



drop by drop on slide till drippings colorless -^ dry 



23.212 Merieux test. 1904 Besson Besson 1904, 247 



REAGENTS REQUIRED: A. DS 23.212 NicoUc 1895, sol. A; B. water 100, iodine 0.5, potas- 

 sium iodide 1, eosin Y 2 

 preparation: Dissolve the iodine and iodide in a few drops of water. Add the eosin dis- 

 solved in 100 water. 

 method: [heat-fixed smear] -^ A, on slide, 10-20 sees. — » rinse -^ B, 5-10 sees. -^ wash 

 dry 



23.212 NicoUe 1896 857, 9 :664 



reagents required: A. DS 12.15 crystal violet; B. ADS 12.2 Nicolle 1895; C. absolute 



ale. 85, acetone 15 

 method: [dried smears] —> A, flooded on slide, 2 mins. -^ quick rinses B, 1 min. — > 



rinse —^ C, till differentiated — > water, to stop differentiation -^ dry — > balsam 



23.212 Schmorl 1928 see DS 21.13 Schmorl 1928b 



23.212 Weiss 1940 11284,26:1518 



reagents required: A. water 80, ale. 20, gentian violet 3; B. ADS 12.2 Lugol (1905); 



C. acetone; -D. 2% magenta in 95% ale. 

 method: [heat-dried smears] — > A, 3-5 mins. —>■ warm water, wash -* B, 3-5 mins. -^ 



warm water — * C, till no more color comes away — > water, wash -^ D, 15 sees. -^ 



water, wash — > dry 



23.212 White and Culbertson 1945 Tech. Bull, 6:53 



REAGENTS REQUIRED: A. 1% Crystal violet; B. 5% sodium bicarbonate; C. water 100, 



mercuric iodide 0.55, potassium iodide 0.45; D. 0.1% safranin 

 method: [heat-dried smears] —> A, 1 ml. on slide -^ B,5 drops, added to'A on slide, leave 

 1 min. -^ drain — > dry — * C, 1 min. -^ wash —> acetone, till no more color comes away 

 — ♦ dry — > D, 10 sees. — > wash — > dry 

 note: The original calls for "a 1% solution of potassium mercuric iodide"; there is no 

 such salt. The pharmacopeial liquor hydraryyri et potassii iodidi contains mercuric 

 iodide and potassium iodide in the proportions given for C above. The same result 

 could be secured by diluting 55 of the pharmacopeial solution to 100. If the solution is 

 prepared from the dry salts, they must first be dissolved in a minimum of water, and 

 then diluted. 



