DS 23.214 DYE STAINS OF SPECIAL APPLICATION 479 



23.214 Doutrelepont see DS 23.214 Giacomi (1896) note 



23.214 Du 1936 test. 1937 Kennedy 20540b, 12 :37 



REAGENTS REQUIRED: A. Water 96, abs. ale. 4, aniline 8, crystal violet 8; B. either 1% 



sodium hydroxide or 5% potassium carbonate or 1% ammonia 

 method: [air-dried smear] — > 8 drops A, on slide — » 8 drops 5% ale. added — » 8 drops B, 



added — + leave 2 mins. — > wash —>■ dry 

 Ri-;('OMMENM)i;i) for: Trcpoiicina pallida. 



23.214 Duperie 1909 11307, 20:1 



formula: water 100, DS 13.13 Giemsa 1902 5, 0.1% sodium carbonate 3 

 method: [smears from formaldehyde-fixed organs] — + dry — > abs. ale, 5 mins. — > dry -+ 

 stain, 5-30 mins. — > wash -^ dry 



23.214 Gelarie 1936 11284,21:1065 



reagents required: A. water 100, sodium chloride 10, zirconium oxychloride 2.5; B. 



10% citric acid; C. water 100, sodium lactate 1.1, crystal violet 0.25; D. water 100. 



potassium iodide 0.5, mercuric iodide 0.5; E. water 100, phenol 0.2, methylene blue 0.2 

 method: [air-dried smears] -^ A, 5 sees. ^ wash —^ B, 10 sees. — > wash -^ C, 30 .sees. — > 



wash -^ D, 5 sees. — » wash —* E, 5 sees. -^ wash -^ blot — * dry 



23.214 Giacomi lest. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 111 



reagents required: A. DS 11.43 Ziehl 1882; B. 0.001% ferric chloride; C. 10% ferric 



chloride 

 method: [smears] -^ A, several mins. 50°C. -^ B, wash — > C, till differentiated — > abs 



ale. — > balsam, via xylene 

 note: Kahlden and Laurent {loc. cit) state that Doutrelepont substitutes a sat. soL 



methyl violet for A above. 



23.214 Giemsa 1905 7276, 31:1026 



REAGENTS REQUIRED: A. Water 100, DS 13.13 Giemsa 1902 1, 0.1% potassium carbon- 

 ate 1 

 method: [heat-dried smear] — + A, 10-30 mins. — » water, wash — > dry 



23.214 Giemsa 1909 7276,35:1751 



reagents required: .4. water 100, DS 13.13 Giemsa 1902 1 



method: [heat-dried smear] -^ A, on slide, warmed to steaming, 15 sees. — > drain -^ re- 

 peat 4 or 5 times -^ water, wash — * dry 



23.214 Goldsworthy and Ward 1942 11431, 54:382 



reagents required: A. water 47.5, methanol 50, acetic acid 2.5, Victoria blue 4R 0.2; 



B. 10% copper sulfate 

 method: [air-dried smears]-^ A, on slide, heated till ale. evaporated —> B, to wash off 



stain -^ blot -+ dry 



23.214 Hoffman 1921 7177,33:1 



reagents required: A. water 100, potassium carbonate 0.01, DS 13.13 Giemsa 7.5; 



B. 25% tannic acid 

 method: [osmic vapor fixed smears]—* water, wash — > A, 1-2 days-* water, wash—* 



B, 1 min. — > water, wash — > dry 



23.214 Harris 1930 19938, 72 :275 



reagents required: A. 1% potassium permanganate; B. 2% methyl violet 

 method: [heat-fixed smear] — > A, 8-10 mins. -^ wash —* B, 8-10 mins. —> wash — ♦ dry 



23.214 Keil 1929 7176,89:1398 



REAGENTS reqttired: A. water 100, glycerol 10, 95% ale. 10, Victoria blue 3, pyronin 



0.9, methyl green 0.1 

 method: [heat-fixed smears] — > A, 3-4 mins. -^ water, wash -^ dry 



23.214 Krauss test. 1942 Langeron Langeron 1942, 827 



REAGENTS REQUIRED: A. Water 100, potassium carbonate 0.005, DS 13.13 Giemsa 5 

 method: [heat-fixed smear] —+ A, on slide, heated to steaming, 4 or 5 times—* water, 

 rinse —> B, I min. -^ water, wash — > dry 



