480 METHODS AND FORMULAS DS 23.214 



23.214 Lipp 1940a 7676, 87 :888 



REAGENTS REQUIRED: A. 1% potassium h^'droxide; B. 0.5% magenta. 

 method: [methanol-fixed smears] -^ ^, on slide — > B, added to A on slide, 3 mins. — > 

 wash —* dry 



23.214 Lipp 1940b 7676,87:888 



reagents required: A. 5% potassium permanganate; B. DS 11.43 Ziehl 1882 10, 



water 90 

 method: [air-dried smear] — * ^, 3 mins. — > wash —* B, 2 mins. — > wash -^ dry 



23.214 Lipp 1940c 7676, 87 :888 



formula: water 81, glycerol 10, abs. ale. 9, Victoria blue 4R 3, pyronin 0.9, methyl 



green 0.1 

 method : [air-dried smear] — > stain, 3 mins. — > wash -^ dry 



23.214 Muhlpfordt 1924 7176, 79:921 



reagents required: A. 3% Victoria blue 

 method: [heat-fixed smears] -^ A, 2-3 mins. -^ wash -^ dry 

 note: The method of Keil 1929 is very commonly referred to Muhlpfordt. 



23.214 Noguchi 1921 11006, 78:191 



reagents required: A. phosphate buffer pH 7.6 90, 40% formaldehyde 10; B. sat. ale. 



sol. {circ. 8%) magenta 

 method: [smear from equal parts specimen and A, left 5 mins. before smearing] -^ dry 



—^ B, 1-5 mins. — > water, wash — * dry 



23.214 One 1938 test. 1942 Langeron Langeron 1942, 830 



reagents required: A. 4% formaldehyde; B. 1% potassium permanganate 

 method: [smears] -^ A, 15 mins. — ^ water, quick wash -^ B, 24 hrs. 60°C. wash -^ dry 

 note: One 1933 (abstract 1935; 20540b, 10 : 112) recommended this process as a mordant 

 before crystal violet. 



23.214 Perrin 1943 Tech. Bull., 4:28 



reagents required: A. water 60, DS 11.43 Ziehl 1882 25, acetic acid 6, 40% formalde- 

 hyde 6 

 method: [heat-fixed smears] — > A, 6 mins. — ^ wash -^ dry 



23.214 Renaux 1923 6630, 89 :420 



reagents required: A. sat. sol. picric acid; B. DS 12.15 crystal violet 

 method: [smears fixed 5 mins. in F 0000.0101 Ruge] — » 95% ale, rinse — » A, 10 mins. -^ 

 water, wash — > B, 10 mins. — > water, wash -^ dry 



23.214 Renaux and Wilmaers 1917 6630, 80 :55 



reagents required: A. 5% tannin; B. DS 11.43 Ziehl 1882 



method: [alcohol-fixed smears]—^ A, warmed to steaming, 30 sees. -^ water, rinse —> 

 B, warmed to steaming, 30 sees. — * water, wash -^ dry 



23.214 Sabrazes 1926 6628, 187 :875 



reagents required: A. DS 11.43 Ziehl 1882 



method: [heat-fixed smears] -^ A, on slide, heated to steaming, 2 or 3 times —> water, 

 wash — > dry 



23.214 Sabrazes 1928 6630, 98:239 



reagents required: A. water 100, tannin 5, phenol 1; B. DS 11.43 Ziehl 1882 

 method: [smears fixed 2 mins. in F 0000.1010 Ruge] — » 95% ale, rinse — > A, warmed to 



steaming, 30 sees. — > water, wash — > B, warmed to steaming on slide 3 or 4 times — * 



water, wash — > dry 



23.214 Sabouraud test. 1904 Besson Besson 1904, 638 



reagents required: A. DS 11.43 Ziehl 1890; B. 1.5% potassium permanganate; C. 2% 



sulfurous acid; D. 1% methylene blue 

 method: [heat-fixed smear] -^ A, 1-2 hrs. — > B, to wash off yl — > C, till decolorized — > 



wash — ♦ D, 1-3 mins. -^ wash — » dry 



