482 METHODS AND FORMULAS DS 23.215 



23.215 Casares-Gil test. 1946 Conn and Darrow Conn and Darrow, 1946, 111A2-14 



REAGENTS REQUIRED: A. water 50, ADS 12.2 Casares-Gil (1946) 50; B. DS 11.43 Ziehl 



1890 

 method: [heat-fixed smears] — > A, on slide, 1 min. -^ water, wash -^ B, on slide, 5 mins. 

 — » wash -^ dry 



23.215 Cerrito test. 1904 Besson Besson 1904, 171 



REAGENTS REQUIRED: A. 25% tannin 60, 5% ferric alum 30, magenta 0.3; B. water 100, 



95% ale. 10, phenol 5, magenta 0.25 

 PREPARATION OF a: Heat ingredients to 100°C. for 6 hours. Cool. Filter. 

 method: [heat-fixed smear] -^ A, 10 mins. — ^ wash -^ dry — > B, on slide, warmed to 



steaming, several sees. — > wash -^ dry 



23.215 van Ermengen 1894 see MS 31.1 van Ermengen 1894 



23.215 Fisher and Conn 1942 20540b, 17:117 



STOCK solutions: I. water 100, tannic acid 7.2, ferric chloride 1.5; II 95% ale. 100, 



magenta 0.5 

 REAGENTS REQUIRED: A. stock I; B. stock I 54, stock II 8, hydrochloric acid 8, 40% 



formaldehyde 30; C. DS 11.43 Ziehl 1890 

 method: [thick smears of washings, incubated 10 mins., from active cultures] —* A, 3}^^ 



mins. -^ drain -^ B, 7 mins. — » water, wash — > C, warmed to steaming, 1 min. — > 



water, wash -^ dry 



23.215 Gemelli test. 1904 Besson Besson 1904, 172 



REAGENTS REQUIRED: A. 0.25% potassiuui permanganate; B. water 100, calcium chloride 



0.75, neutral red 0.05 

 method: [heat-fixed smear] — > A, 10-20 mins. — > wash -^ B, 15-30 mins. -^ wash — » dry 



23.215 Gray 1926 11056, 12:273 



reagents required: A. ADS 12.2 Gray 1926; B. DS 11.43 Ziehl 1890 

 method: [heat-fixed smears] —> A, on slide, 10 mins. — > water, wash —* B, 5 mins. -^ 

 water, wash -^ dry 



23.215 Inouye 1924 test. 1942 Langeron Langeron 1942, 1219 



REAGENTS REQUIRED: A. DS 23.215 Loffler 1890 (sol. ^4); B. water 75, potassium alum 3, 



sat. ale. sol. (circ. 10%) crj'stal violet 15 

 method: [heat- fixed smears] — » A, on slide, warmed to steaming, 1 min. — > water, wash 



-^ B, on slide, warmed to steaming, 1 min. -^ wash -^ dry 



23.215 Kendall 1902 see DS 23.215 Pittfield (1910) 



23.215 Kulp 1926 20540b, 1 :60 



REAGENTS required: A. Water 75, 95% ale. 5 tannic acid 10, ferrous sulfate 4, magenta 



0.5; B. 0.6% magenta in sat. sol. aniline 

 preparation of a: Dissolve the tannic acid in 50 water. Add the ferrous sulfate dis- 

 solved in 25 and the dye dissolved in the ale. Leave 18-24 hours. Filter or centrifuge. 

 method: [air-dried smears] — > A, 15 mins. — » thorough wash —> B, 15 mins. — > wash — + 

 dry 



23.215 Liefson 1930 11056. 20:203 



formula: water 80, 95% ale. 36, potassium alum 2, tannic acid 4, magenta 0.6 

 preparation: To the alum dissolved in 40 water add the tannic acid dissolved in 20. 



Add 20 water and 30 ale. To this add the dye dissolved in 6 ale. 

 method: [air-dried smear] -^ stain 10 mins. -^ wash —> [DS 11.44 Loffler 1890, if 



counterstain desired] -^ dry 



23.215 Loffler 1890a 23684, 7:625 



reagents required: .4. water 100, tannic acid 12, ferrous sulfate 5, magenta .03; B. 



water 100, aniline and crystal violet, each to sat., sodium carbonate .001 

 preparation of a: To the tannic acid dissolved in 60 water, add the ferrous sulfate dis- 

 solved in 30. To this add dye dissolved in 10 water. 



