DS 23.216 DYE STAINS OF SPECIAL APPLICATION 485 



23.216 Aladar-Anjeszky test. 1904 Besson Besson 1904, 165 



REAGENTS REQUiREu: A. 0.5% hydrochloric acid; B. DS 11.43 Ziehl 1890; C. 4% sulfuric 



acid; D. \% methylene blue 

 method: [air-dried smear] -^ A,Z mins. in beaker at 80°C. — » wash -^ dry -> flame — » 

 B, on slide, heated to steaming, 2 or 3 changes -^ C, till decolorized — > wash — > D, 1 

 min. — > wash — > dry 



23.216 Anjeszky 1909 23G84, 23 



REAGENTS REQUIRED: A. 0.5% hydrochloric acid; B. DS 11.43 Ziehl 1890; C. 3%, sulfuric 



acid; D. 1% malachite green 

 method: [dried, but not flamed, smear] -^ A, heated to steaming, 3-4 mins. -* wash —y 



dry, -* flame — > B, warmed to steaming, 1-2 mins. -♦ wash -> C, till decolorized -+ 



thorough wash — > D, 1-2 mins. — * wash — » dry 



23.216 Ashby 1938 19938,87:443 



REAGENTS REQUIRED: A. 5% malachite green; B. 0.5%, safranin 



method: [heat-fixed smear] —> A, on slide, heated over steam bath, 1 min. ^ water, 

 wash — > dry 



23.216 Besson 1904a Besson 1904, 164 



reagents required: A. 5% chromic acid; B. DS 12.15 gentian violet 

 method: [heat-fixed smears] —> A, on slide, 4-5 mins. -^ wash — > B, on slide, 15-20 

 mins. — > wash — ♦ dry 



23.216 Besson 1940b ' Besson 1904, 165 



REAGENTS REQUIRED: A. DS 11.43 Ziehl 1882; 25% nitric acid; C. 1% methylene blue 

 method: [heat-fixed smear] —> A, on slide, heated to steaming, 3-4 mins. — > wash —* B, 



few sees. — >• wash — > C, on slide, 30 sees. — » wash -^ dry 

 result: spores, red; bacteria, blue. 



23.216 Bitter 1913 23684, 68:227 



REAGENTS REQUIRED: A. 1% formaldehyde; B. DS 11.44 Loffler 1890; C. water 50, sat. 



ale. sol. {circ. 3%) safranin 50 

 method: [heat-fixed smears] -^ A, 10-20 mins. — * water, wash — > B, on slide, heated to 

 boiling, 2-3 times, — > water, thorough wash -* C, 30 sees. -^ water, wash -^ dry 



23.216 Botelho 1918 6630,81:183 



formula: water 50, acetic acid 50, light green 4, acid fuchsin 2 

 method: [heat-fixed smears]-^ stain, heated to steaming, 3 to 4 times—* water, wash 



till greenish -^ repeat staining cycle till color sufficiently deep -^ wash — > dry 

 result: spores red on green. 



23.216 Bruner and Edwards 1939 11284, 25:543 



REAGENTS REQUIRED: A. 5% malachite green; B. 0.5% safranin 

 method: [heat-fixed smears] — > A, 5 mins. -^ wash — >• B, 10 sees. — > wash -^ dry 



23.216 Dorner 1926 11988, 6:8 



REAGENTS REQUIRED: A. DS 11.43 Ziehl 1890; B. 10% nigrosin 

 method: [suspension of organism in test tube] — » add equal volume A, heat to 100°C., 



10 mins. -^ place loopful of mixture on slide, add loopful of B, smear -^ dry 

 note: Snyder 1934 (20540b, 9:71) applies A to smear under saturated blotting-paper 

 cover and counterstains with B. 



23.216 Dutton 1928 20540b, 3:140 



REAGENTS REQUIRED: A. phosphatc buffer pH 7.6; B. DS 13.12 Wright 1910 (0.15% in 



methanol) 

 method: Prepare thick suspension of bacteria in A. Add 0.1 B. Seal tube and heat to 

 100°C. for 10 mins. Cool, unseal tube, and make smear. 



23.216 Fraenkell922 23684,89:106 



REAGENTS REQUIRED: A. 20% tannin; B. DS 11.43 Ziehl 1890; C. 5% sulfuric acid; D. 

 0.1% methylene blue. 



