DS 23.216-DS 23.217 DYE STAINS OF SPECIAL APPLICATION 487 



23.216 Schaeffer and Fulton 1933 19938, 77:194 



REAGENTS REQUIRED: A. 5% malachite green; B. 0.5% safranin O 

 method: [heat-fixed smears] — > A, poured on slide, heated and reheated to steaming, 3-4 



times — > water, wash — > B, 30 sees. — » water, wash -^ dry 

 result: spores green on red. 



23.216 Shapiro 1944 20540b, 19:65 



REAGENTS REQUIRED: A. 5% malachite green; B. 0.5% safranin 

 method: [mix 2-3 drops bacterial suspension with equal amount A. Heat in boiling 



water bath 15-20 mins. Make smear and heat fix.] -^ wash, 10 sees. -^ B, 1 min. —> 



wash — » blot — > dry 



23.216 Snyder 1934 see DS 23.21G Dorner 1926 (note) 



23.216 Tribondeau 1917 6630, 80:880 



REAGENTS REQUIRED: A. ADS 12.2 Lugol (1905); B. DS 12.15 crystal violet; C. 0.2% 



Bismarck brown 

 method: [heat-fixed smears] —> A, heated to steaming, 2-3 times —»• blot — > B, heated 



to steaming, 2-3 times -^ water, wash 

 result: spores violet on brown. 



23.216 Zeeti 1935 20540b (abs. 1930) 11 :87 

 reagents required: A. water 100, eosin Y 5, phenol 5, iodine 2, potassium iodide 1; B. 



0.075% methylene blue in 5%, ale. 

 method: [heat-fixed smears] — > A, heated to boiling, 5 mins. — > wash — > B, 1-2 mins. -* 

 wash — > dry 



23.317 Capsule Stains 



The standard method for staining the capsule of capsulated bacteria is still that of 

 Hiss 1905, though many other methods have been developed. One of the most inter- 

 esting of these is that of Huntoon 1917, in which the capsule is first caused to absorb a 

 solution of nutrose, w^hich is itself subsequent]}' brought into prominence by a chemical 

 reaction specific to it. The other methods largel}^ rely on mordanting with formaldehj'de 

 and subsequently carrying out a standard Gram procedure with counterstaining for the 

 mordanted capsule. 



23.217 Anthony 1931 see DS 23.207 Hiss 1905 (note) 



23.217 Besson 1904 Besson 1904, 166 



reagents required: A. water 100, crystal violet 0.5, acetic acid 1 

 method: [heat-fixed smear] -^ A, I min. — > rinse — > dry 



23.217 Boni test. 1928 Schmorl Schmorl 1928, 359 



reagents required: A. V 21.1 Mayer 1884; B. DS 11.43 Ziehl 1890 

 method: [place a drop of A on slide. Mix in a trace of bacterial culture. Make smear. 

 Dry. Flame] — > B, warmed to steaming, 1 min. — > wash — > dry 



23.217 Buerger 1904 test. 1928 Zinnser and Bayne-Jones 



Zinnser and Bayne-Jones, 1928, 847 

 reagents required: A. ADS 12.2 Gram 1882; B. DS 23.211 Ehrlich 1882; C. 2% so- 

 dium chloride 

 method: [F 3700.0000 fixed smear] ^ wash ^ 95 % ale. — > wash — > A, 1-3 mins. ^ 

 95% ale, wash -^ dry — > B, few sees. — > C, wash —* examine in C 



23.217 Churchman and Emelianoff 1932 16913, 29:514 



reagents required: .4. DS 13.12 Wright 1910; B. phosphate buffer pll 6.4 

 method: [air-dried smear] —* A, on slide, till evaporation changes color from blue to 

 pink —>■ B, wash —* dry 



