DS 23.217-DS 23.218 DYE STAINS OF SPECIAL APPLICATION 489 



23.217 Schmorl 1928 Schmorl 1928, 359 



REAGENTS REQUIRED: A. 1% potassium hydroxide; B. 2% crystal violet 

 method: [heat-fixed smears] -^ A, 3-5 mins. -^ quick rinse —>■ B, 3-4 mins. -^ wash — > 

 dry 



23.217 Tyler 1946 see DS 23.217 Hiss 1905 (note) 



23.217 Wadsworth 1906 11250,3:610 



REAGENTS REQUIRED: A. 40% formaldehyde; B. DS 23.211 Ehrlich 1882; C. ADS 12.2 



Gram 1882; D. 0.1% magenta 

 method: [heat-fixed smears] -* A, 2-5 mins. -^ water, rinse -^ B,2 mins. -♦ C, 2 mins. 



-^95% ale, till no more color comes away -^ D, 30 sees, dry 



23.217 Welch 1892 10919,3:81 



REAGENTS REQUIRED: A. acetic acid ; B. DS 23.211 Ehrlich 1882 (sol. A); C. 2% sodium 



chloride 

 method: [dried, not heated, smear] -^ A, few sees. -^ B, drained and renewed on slide 



till A removed -^ B, fresh solution, 2 mins. -^ C, wash — > examine 



SS.218 Diphtheria Bacilli 



Diphtheria bacilli themselves are not susceptible to differential staining. Diagnosis 

 by microscope is dependent upon bringing into prominence the granules w^hich they 

 uniquely have within them. These granules have much the same reactions as any other 

 minute body, hence the methods for their demonstration most strongly resemble those 

 given either for the demonstration of Rickettsiae, or for mitochondria and other cell 

 inclusions. The original method of Neisser 1897 is still the most widely employed and 

 all these techniques are occasionally referred to as Neisser stains even though they may 

 bear no relation at all to the original description. 



23.218 Albert 1921 11006, 76:240 



REAGENTS REQUIRED: A. Water 100, 95% ale. 2, acetic acid 1, methyl green 0.02, toluidine 



blue 0.15; B. ADS 12.2 Gram 1882 

 method: [heat-fixed smears] — > A, 5 mins. -^ blot -^ B, 1 min. -^ water, rinse -> blot -^ 



dry 

 note: Laybourn 1924 (11006, 83:121) substitutes 0.2 malachite green for 0.02 methyl 



green in A above. 



23.218 Ambrosioni 1940 988, 60 :228 



REAGENTS REQUIRED: A. Water 95, acetic acid 5, crystal violet 0.33; B.2% chrysoidin; C. 



ADS 12.2 Lugol 99, lactic acid 1 

 method: [heat-fixed smear] -* A, 10-15 sees. -^ B, 20-30 sees. -^ wash — > C, 5 sees. -^ 



wash —* dry 



23.218 Beauverie 1917 6630, 80 :609 



REAGENTS REQUIRED: A. DS 11.44 Loffler 1890; B. ADS 12.2 Lugol 

 method: [alcohol-fixed smears] — > A, 2-3 mins. -^ wash — > B, 2-3 mins. -^ wash — > dry 

 note: Mallory 1922 (4349, 8:110) recommends counterstaining in 1% eosin after this 

 technique. 



23.218 Cowdry 1943 Cowdry 1943, 62 



reagents required: A. DS 23.218 Neisser 1897 (sol. A) 60, 0.3% crystal violet 30; 



B. 0.3% chrysoidin 

 method: [heat-fixed smear] -^ yl, on slide, H to 1 ^"^- -^ water, wash -> B, on slide, 



30 sees. -^ water, wash -^ dry 

 note: See also DS 23.218 Neisser 1897 (note). 



23.218 Kinyoun 1915 617, 5:246 



formula: water 120, acetic acid 1, 95% ale. 5, toluidine blue 0.01, azur A 0.01, meth- 

 ylene blue 0.01 

 preparation: Dissolve dyes in ale. Add acidified water. 

 method: [heat-fixed smears] -^ stain, 5 mins. — » water, wash -^ dry 

 result: granules red on blue. 



