490 METHODS AND FORMULAS DS 23.218-DS 23.219 



23.218 Kemp 1931 11284, 16:593 



REAGENTS REQUIRED: A. ADS 12.2 Graiu 1884; B. DS 11.44 Loffler 1890; C. 1% safranin 

 method: [heat-fixed smears] ~^ A, 1 min. -> wash -^ B, 20-30 sees. —> wash -^ C, 10-15 

 sees. -^ wash — * dry 



23.218 Laybourn 1924 see DS 23.218 Albert 1921 (note) 



23.218 Ljubinsky test. 1946 Conn and Darrow cit. Blumenthal and Lipskerow 



Conn and Darrow 194G, 111A2-12 

 REAGENTS REQUIRED: A. Water 95, acetic acid 5, crystal violet 0.25; B. 0.1% Bismarck 



brown Y 

 method: [heat-fixed smears] -^ A, 12 to 2 mins. -^ water, wash -^ B, 30 sees. -* water, 



wash -^ dry 

 result: granules, blue-black on yellow. 



23.218 Mallory 1922 see DS 23.218 Beauverie 1917 (note) 



23.218 Neisser 1897 23454, 24:448 



reagents required: A. 95% ale. 2, methylene blue 0.1, water 95, acetic acid 5; B. 



0.2% Bismarck brown 

 method: [heat-fixed smears]^ A, 1-3 sees. -^ water, wash -^ B, 3-5 sees. -^ water, 



wash — > dry 

 note: The timing given is that of the original paper. Modern practice calls for longer 



staining. Cowdry 1943, 62, for example, recommends l^ to 1 minute in A and 30 



seconds in B. 



23.218 Ponder lest. 1920 Stitt Stitt 1920, 55 



formula: water 100, 95% ale. 2, acetic acid 1, toluidine blue 0.02 



method : [heat-fixed smear on coverslip] —* stain, dropped on coverslip -* examine as 

 hanging drop 



23.218 Ryn 1940 11796,17:53 



REAGENTS REQUIRED: A. sat. aq. sol. potassium aluminate {not alum) 5, sat. ale. sol. 



methyl violet 1 

 method: [heat-fixed smear] — * stain, 2-3 mins. -^ wash — > dry 



23.218 Stottenberg 1926 7276,20:426 



formula: water 100, acetic acid 3, abs. ale. 3, malachite green 0.25, toluidine blue 0.05, 



hematoxylin 0.01 

 method: [dry smear] — > stain, 1 min. — * wash — > dry 



23.218 Tribondeau and Dubreuil 1917 6630, 80:331 

 REAGENTS REQUIRED: A. DS 11.44 Nicolle (1942); B. 0.2% Bismarck brown 

 method: [alcohol-fixed smear] — > A, 5 mins. — > water, wash — » 1-2 mins. — > water, wash 



-^ dry 



23.219 Other Methods for Bacterial Smears 



23.219 Broadhurst and Paley 1939 11023, 94:525 



formula: 95% ale. 62, tetrachlorethane 40, sulfuric acid 0.4, methylene blue 1, magenta 



0.08 

 preparation: Mix 50 ale. with tetrachlorethane and acid. Heat to 55°C. Add methylene 



blue and shake to solution. Dissolve magenta in 10 ale. and add to blue. 

 method: [thin film of milk dried to slide] — > stain, on slide, 15 sees. — > drain -^ dry — ♦ 



wash till faint pink -^ dry 

 recommended for: bacteria in milk. 



23.219 Bruner and Edwards 1940 11284, 25:543 



REAGENTS REQUIRED: A. 5% malachite green; B. 0.5% safranin 



method: [heat-fixed blood smear] ^ ^4, 5 mins. — ^ rinse — * B, 10 sees. — > wash —> dry 

 recommended for: demonstration of bacteria in leukocytes. 



